Elsevier S & T

Amyloid, Prions, and Other Protein Aggregates, Part B

Wetzel, Ronald

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Table of contents
  • Title Pageiii
  • Copyright Pageiv
  • Table of Contentsv
  • Contributors to Volume 412ix
  • Prefacexiii
  • Volumes in Seriesxvii
  • Section I: Characterization of Protein Deposition In Vivo and Ex Vivo1
  • Chapter 1. Protein Misfolding Cyclic Amplification for Diagnosis and Prion Propagation Studies3
  • Chapter 2. Fractionation of Prion Protein Aggregates by Asymmetrical Flow Field-Flow Fractionation21
  • Chapter 3. Analysis of Amyloid Aggregates Using Agarose Gel Electrophoresis33
  • Chapter 4. Characterization of Systemic Amyloid Deposits by Mass Spectrometry48
  • Chapter 5. Proteomics of Polyglutamine Aggregates63
  • Chapter 6. Merger of Laser Capture Microdissection Mass Spectrometry: A Window into the Amyloid Plaq77
  • Chapter 7. MALDI MS Imaging of Amyloid94
  • Chapter 8. Imaging Polyglutamine Deposits in Brain Tissue106
  • Chapter 9. X-34 Labeling of Abnormal Protein Aggregates During the Progression of Alzheimer’s Dise123
  • Chapter 10. Visualizing Pathology Deposits in the Living Brain of Patients with Alzheimer’s Diseas144
  • Chapter 11. Micro-Imaging of Amyloid in Mice161
  • Section II: Cell and Animal Models of Amyloid Formation and Toxicity183
  • Chapter 12. An Ef.cient Protein Transformation Protocol for Introducing Prions into Yeast185
  • Chapter 13. Screening for Genetic Modifiers of Amyloid Toxicity in Yeast201
  • Chapter 14. Searching for Anti-Prion Compounds: Cell-Based High-Throughput In Vitro Assays and Anima223
  • Chapter 15. A Drosophila Model of Alzheimer’s Disease234
  • Chapter 16. Modeling Polyglutamine Pathogenesis in C. elegans256
  • Section III: Computational Approaches and Theory283
  • Chapter 17. Nucleation: The Connections Between Equilibrium and Kinetic Behavior285
  • Chapter 18. Amyloid Fibril Structure Modeling Using Protein Threading and Molecular Dynamics Simulat300
  • Chapter 19. Ab initio Discrete Molecular Dynamics Approach to Protein Folding and Aggregation314
  • Chapter 20. Computational Approaches to Fibril Structure and Formation338
  • Author Index367
  • Subject Index395
Book details
  • Vendor Elsevier S & T
  • SKU 9780121828172
  • ISBN-13 9780080522548
  • Author Wetzel, Ronald
  • Category Science
  • Subject Biochemistry

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The ability of polypeptides to form alternatively folded, polymeric structures such as amyloids and related aggregates is being increasingly recognized as a major new frontier in protein research. This new volume of Methods in Enzymology along with Part C (volume 413) on Amyloid, Prions and other Protein Aggregates continue in the tradition of the first volume (309) in containing detailed protocols and methodological insights, provided by leaders in the field, into the latest methods for investigating the structures, mechanisms of formation, and biological activities of this important class of protein assemblies.

* Presents detailed protocols
* Includes troubleshooting tips
* Provides coverage on structural biology, computational methods, and biology