Antisense Technology, Part A, General Methods, Methods of Delivery, and RNA Studies
Abelson, John N.; Simon, Melvin I.
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Table of contents
- Table of Contentsv
- Contributors to Volume 313ix
- Prefacexiii
- Volumes In Seriesxv
- Section I: General Methods1
- Chapter 1. Progress in Antisense Technology: The End of the Beginning3
- Chapter 2. Basic Principles of Using Antisense Oligonucleotides in Vivo46
- Chapter 3. Polyethyleneimine-Mediated Transfection to Improve Antisense Activity of 3' -Capped Phosp56
- Chapter 4. Design of Antisense and Triplex-Forming Oligonucleotides74
- Chapter 5. Chimeric Oligodeoxynucleotide Analogs: Chemical Synthesis, Purification, and Molecular an95
- Chapter 6. Evaluation of Antisense Mechanisms of Action135
- Chapter 7. Physiology and Molecular Biology Brought to Single-Cell Level143
- Chapter 8. Antisense Properties of Peptide Nucleic Acid156
- Chapter 9. Synthesis of Oligonucleotide Conjugates in Anhydrous Dimethyl Sulfoxide164
- Chapter 10. Gene Switching: Analyzing Broad Range of Mutations Using Steric Block Antisense Oligonuc174
- Chapter 11. Vesicular Stomatitis Virus as Model System for Studies of Antisense Oligonucleotide Tran189
- Chapter 12. Purification of Antisense Oligonucleotides203
- Chapter 13. Boranophosphate Backbone: A Mimic of Phosphodiesters, Phosphorothioates, and Methyl Phos226
- Chapter 14. Intracellular Distribution of Digoxigenin-Labeled Phosphorothiate Oligonucleotides257
- Chapter 15. Use of Minimally Modified Antisense Oligonucleotides for Specific Inhibition of Gene Exp268
- Section II: Methods of Delivery285
- Chapter 16. Determination of Cellular Internalization of Fluorescent Oligonucleotides287
- Chapter 17. Intrabody Tissue-Specific Delivery of Antisense Conjugates in Animals: Ligand–Linker A297
- Chapter 18. Lipid-Based Formulations of Antisense Oligonucleotides for Systemic Delivery Application322
- Chapter 19. In Vitro Transport and Delivery of Antisense Oligonucleotides342
- Chapter 20. In Vitro and in Vivo Delivery of Antisense Oligodeoxynucleotides Using Lipofection: Appl359
- Chapter 21. Preparation and Application of Liposome-Incorporated Oligodeoxynucleotides372
- Chapter 22. Cell-Specific Optimization of Phosphorothioate Antisense Oligodeoxynucleotide Delivery b388
- Section III: RNA Studies399
- Chapter 23. Intracellular Expression and Function of Antisense Catalytic RNAs401
- Chapter 24. Selective Degradation of Targeted mRNAs Using Partially Modified Oligonucleotides420
- Chapter 25. Reversible Depletion of Specific RNAs by Antisense Oligodeoxynucleotide-Targeted Degrada436
- Chapter 26. Inactivation of Gene Expression Using Ribonuclease P and External Guide Sequences442
- Chapter 27. Disruption of mRNA-RNP Formation and Sorting to Dendritic Synapses by Antisense Oligonuc456
- Chapter 28. Antisense RNA and DNA in Escherichia coli467
- Chapter 29. Utilization of Properties of Natural Catalytic RNA to Design and Synthesize Functional R485
- Chapter 30. Antisense Oligonucleotides and RNAs as Modulators of pre-mRNA Splicing506
- Chapter 31. Selective RNA Cleavage by Isolated RNase L Acti- vated with 2–5A Antisense Chimeric Ol522
- Author Index535
- Subject Index567
Book details
- Vendor Elsevier S & T
- SKU 9780121822149
- ISBN-13 9780080496702
- Author Abelson, John N.; Simon, Melvin I.
- Category Medical
- Subject Biochemistry
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Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods that inhibit gene expression permit studies which probe the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedical-oriented research.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
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