Elsevier S & T

Antisense Technology, Part B: Applications: Applications

Abelson, John N.; Simon, Melvin I.

In stock
No reviews
74.000 KD inc. VAT
License
Table of contents
  • Table of Contentsv
  • Contributors to Volume 314ix
  • Prefacexv
  • Volumes in Seriesxvii
  • Section I: Antisense Receptor Targets1
  • Chapter 1. In Vivo Modulation of G Proteins and Opioid Receptor Function by Antisense Oligodeoxynucl3
  • Chapter 2. Targeting Brain GABAA Receptors with Antisense Oligonucleotides: Implications for Epileps20
  • Chapter 3. Delivery of Antisense DNA by Vectors for Prolonged Effects in Vitro and in Vivo32
  • Chapter 4. Antisense Mapping: Assessing Functional Significance of Genes and Splice Variants51
  • Chapter 5. In Vitro and in Vivo Effects of Antisense on alpha2-Adrenoceptor Expression61
  • Chapter 6. Design and Efficacy of a Serotonin-2A Receptor Antisense Oligodeoxynucleotide76
  • Chapter 7. Reduction of Neurotransmitter Receptor and G-Protein Expression in Vivo and in Vitro by A90
  • Chapter 8. Use of Expression of Antisense mRNA for Convertases 1 and 2 in Prohormone Processing103
  • Section II: Antisense Neuroscience Targets119
  • Chapter 9. Strategies for the Design and Delivery of Antisense Oligonucleotides in Central Nervous S121
  • Chapter 10. Use of Antisense Expression Plasmids to Attenuate G-Protein Expression in Primary Neuron136
  • Chapter 11. Gene Functional Analysis in Nervous System148
  • Chapter 12. RNA Mapping: Selection of Potent Oligonucleotide Sequences for Antisense Experiments168
  • Chapter 13. Effects of Centrally Administered Antisense Oligodeoxynucleotides on Feeding Behavior an184
  • Chapter 14. Blockade of Neuropathic Pain by Antisense Targeting of Tetrodotoxin-Resistant Sodium Cha201
  • Chapter 15. Antisense Approach for Study of Cell Adhesion Molecules in Central Nervous System213
  • Chapter 16. Sequence Design and Practical Implementation of Antisense Oligonucleotides in Neuroendoc223
  • Chapter 17. Localization of Oligonucleotides in Brain by in Situ Hybridization238
  • Chapter 18. Use of Antisense Oligonucleotides in Human Neuronal and Astrocytic Cultures247
  • Chapter 19. Pharmacokinetic Properties of Oligonucleotides in Brain261
  • Chapter 20. Antisense Oligonucleotides: Preparation and in Vivo Application to Rat Brain275
  • Chapter 21. Application of Antisense Techniques to Characterize Neuronal Ion Channels in Vitro290
  • Section III: Antisense in Nonneuronal Tissues311
  • Chapter 22. Antisense Inhibition of Sodium–Calcium Exchanger313
  • Chapter 23. Targeted Delivery of Antisense Oligonucleotides to Parenchymal Liver Cell in Vivo324
  • Chapter 24. Antisense Methods for Discrimination of Phenotypic Properties of Closely Related Gene Pr342
  • Chapter 25. Evaluation of Biological Role of c-Jun N-Terminal Kinase Using an Antisense Approach363
  • Chapter 26. Role of Antisense in Kidney Cells378
  • Chapter 27. Use of Antisense Techniques in Rat Renal Medulla389
  • Chapter 28. Antisense Approaches to in Vitro Organ Culture401
  • Chapter 29. In Vivo and in Vitro Antiproliferative Effects of Antisense Interleukin 10 Oligonucleoti411
  • Chapter 30. Inhibition of c-ABL Expression in Hematopoietic Progenitor Cells Using Antisense Oligode429
  • Chapter 31. Cellular Pharmacology of Antisense Oligodeoxynucleotides440
  • Chapter 32. Application of Antisense Oligodeoxynucleotides for Suppression of Na+/Ca2+ Exchange454
  • Chapter 33. Optimizing Efficacy of Antisense Oligodeoxynucleotides Targeting Inhibitors of Apoptosis477
  • Section IV: Antisense in Therapy491
  • Chapter 34. In Vitro and in Vivo Modulation of Transforming Growth Factor beta1 Gene Expression by A493
  • Chapter 35. Analysis of Cancer Gene Functions through Gene Inhibition with Antisense Oligonucleotide499
  • Chapter 36. Dosimetry and Optimization of in Vivo Targeting with Radiolabeled Antisense Oligodeoxynu506
  • Chapter 37. Antisense Oligonucleotide Therapy of Hepadnavirus Infection524
  • Chapter 38. Preclinical Antisense DNA Therapy of Cancer in Mice537
  • Chapter 39. Retrovirally Mediated Delivery of Angiotensin II Type 1 Receptor Antisense in Vitro and581
  • Author Index591
  • Subject Index633
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822156
  • ISBN-13 9780080496719
  • Author Abelson, John N.; Simon, Melvin I.
  • Category Medical
  • Subject Oncology

Do you have questions about this book?

Ask an expert!

Description eBook delivery & access Reviews
Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods which inhibit gene expression permit studies probing the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedically-oriented research.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.