Elsevier S & T

Apoptosis

Abelson, John N.; Simon, Melvin I.

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 322ix
  • Prefacexiii
  • Volumes in Seriesxv
  • Section I: Measuring Apoptosis and Apoptosis-Induced Endonucleases1
  • Chapter 1. Detection of DNA Cleavage in Apoptotic Cells3
  • Chapter 2.Detection of Apoptosis by Annexin V Labeling15
  • Chapter 3. Analysis of Apoptotic Cells by Flow and Laser Scanning Cytometry18
  • Chapter 4. Quantitative Measurement of Apoptosis Induced by Cytotoxic T Lymphocytes40
  • Chapter 5. Apoptotic Nuclease Assays47
  • Section II: Measuring Apoptosis in Lower Organisms63
  • Chapter 6. Analysis of Programmed Cell Death and Apoptosis in Drosophila65
  • Chapter 7. Programmed Cell Death in the Nematode Caenorhabditis elegans76
  • Section III: Proteases Involved in Apoptosis and Their Inhibitors89
  • Chapter 8. Caspase Assays91
  • Chapter 9. Determination of Caspase Specificities Using a Peptide Combinatorial Library100
  • Chapter 10. Criteria for Identifying Authentic Caspase Substrates during Apoptosis110
  • Chapter 11. Purification and Use of Granzyme B125
  • Chapter 12. Viral Caspase Inhibitors CrmA and p35143
  • Chapter 13. Purification and Use of Recombinant Inhibitor of Apoptosis Proteins as Caspase Inhibitor154
  • Chapter 14. Monitoring Activity of Caspases and Their Regulators in Yeast Saccharomyces cerevisiae162
  • Section IV: Cell-Free Systems for Monitoring Steps in Apoptosis Pathways175
  • Chapter 15. In Vitro Assays for Caspase-3 Activation and DNA Fragmentation177
  • Chapter 16. Cell-Free Apoptosis in Xenopus laevis Egg Extracts183
  • Chapter 17. Cytofluorometric Quantification of Nuclear Apoptosis Induced in a Cell-Free System198
  • Section V: Mitochondria and Apoptosis203
  • Chapter 18. Purification of Mitochondria for Apoptosis Assays253
  • Chapter 19. Quantitation of Mitochondrial Transmembrane Potential in Cells and in Isolated Mitochond207
  • Chapter 20. Nitrogen Cavitation for Cell Disruption to Obtain Mitochondria from Cultured Cells212
  • Chapter 21. Apoptosis-Related Activities Measured with Isolated Mitochondria and Digitonin-Permeabil221
  • Chapter 22. Assays for Cytochrome c Release from Mitochondria during Apoptosis234
  • Chapter 23. Purification and Liposomal Reconstitution of Permeability Transition Pore Complex242
  • Section VI: Bcl-2 Family Proteins252
  • Chapter 24. Monitoring Interactions of Bcl-2 Family Proteins in 96-Well Plate Assays255
  • Chapter 25. Analysis of Dimerization of Bcl-2 Family Proteins by Surface Plasmon Resonance266
  • Chapter 26. Measuring Pore Formation by Bcl-2 Family Proteins274
  • Chapter 27. Assays for Studying Bax-Induced Lethality in the Yeast Saccharomyces cerevisiae283
  • Chapter 28. Exploiting the Utility of Yeast in the Context Programmed Cell Death297
  • Section VII: Studying Receptors and Signal Transduction Events Implicated in Cell Survival and Cell323
  • Chapter 29. Production of Recombinant TRAIL and TRAIL Receptor: Fc Chimeric Proteins325
  • Chapter 30. Expression of Lymphotoxins and Their Receptor–Fc Fusion Proteins by Baculovirus345
  • Chapter 31. Analysis of the CD95 (APO-1/Fas) Death-Inducing Signaling Complex by High-Resolution Two363
  • Chapter 32. Measurement of Ceramide Levels by the Diacylglycerol Kinase Reaction and by High-Perform373
  • Chapter 33. Measurement of Ceramide Synthase Activity378
  • Chapter 34. Measurement of Sphingomyelinase Activity382
  • Chapter 35. Assays for JNK and p38 Mitogen-Activated Protein Kinases388
  • Chapter 36. Assaying for IKB Kinase Activity393
  • Chapter 37. Assays for Akt400
  • Section VIII: Other Methods411
  • Chapter 38. Measurement of Cellular Oxidation, Reactive Oxygen Species, and Antioxidant Enzymes duri413
  • Chapter 39. Volume Regulation and Ion Transport during Apoptosis421
  • Chapter 40. Assays for Transglutaminases in Cell Death433
  • Chapter 41. Anoikis472
  • Chapter 42. Transient Transfection Assay of Cell Death Genes480
  • Chapter 43. Sindbis Virus Vector System for Functional Analysis of Apoptosis Regulators492
  • Chapter 44. Transduction of Full-Length Tat Fusion Proteins Directly into Mammalian Cells: Analysis508
  • Author Index523
  • Subject Index555
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822231
  • ISBN-13 9780080496771
  • Author Abelson, John N.; Simon, Melvin I.
  • Category Science
  • Subject Biochemistry

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Volume 322 of Methods in Enzymology is dedicated to apoptosis. Major topics covered include measuring apoptosis and apoptosis-induced endonucleases, measuring apoptosis in lower organisms, proteases involved in apoptosis and their inhibitors, cell free systems for monitoring steps in apoptosis pathways, mitochondria and apoptosis, bCl-2 family proteins, and studying receptors and signal transduction events implicated in cell survival and cell death.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.