Elsevier S & T

Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification: Gene Expression and Protein Purification

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 326ix
  • Prefacexiii
  • Volumes in Seriesxv
  • Section I: Historical Overview1
  • Chapter 1. The All Purpose Gene Fusion3
  • Section II: Gene Fusions as Reporters of Gene Expression in Prokaryotic Cells9
  • Chapter 2. A Practical Guide to the Construction and Use of lac Fusions in Escherichia coli11
  • Chapter 3. Tagging Exported Proteins Using Escherichia coli Alkaline Phosphatase Gene Fusions35
  • Chapter 4. Applications of Gene Fusions to Green Fluorescent Protein and Flow Cytometry to the Study47
  • Chapter 5. IVET and RIVET: Use of Gene Fusions to Identify Bacterial Virulence Factors Specifically73
  • Chapter 6. Identification of Exported Bacterial Proteins via Gene Fusions to Yersinia pseudotubercul97
  • Section III: Gene Fusions as Reporters of Gene Expression in Eukaryotic Cells105
  • Chapter 7. Use of Imidazoleglycerolphosphate Dehydratase (His3) as a Biological Reporter in Yeast107
  • Chapter 8. Use of Fusions to Human Thymidine Kinase as Reporters of Gene Expression and Protein Stab120
  • Chapter 9. Use of Fusions to Thymidine Kinase134
  • Chapter 10. In Vivo Analysis of lacZ Fusion Genes in Transgenic Drosophila melanogaster146
  • Chapter 11. Utility of the Secreted Placental Alkaline Phosphatase Reporter Enzyme159
  • Chapter 12. Fusions to Imidazopyrazinone-Type Luciferases and Aequorin as Reporters165
  • Chapter 13. Novel Methods for Chemiluminescent Detection of Reporter Enzymes175
  • Chapter 14. Fusions to Chloramphenicol Acetyltransferase as a Reporter202
  • Chapter 15. Fusions to β-Lactamase as a Reporter for Gene Expression in Live Mammalian Cells221
  • Section IV: Functional Tags for Biochemical Purification243
  • Chapter 16. Purification of Proteins Using Polyhistidine Affinity Tags245
  • Chapter 17. Generating Fusions to Glutathione S-Transferase for Protein Studies254
  • Chapter 18. Use of the Strep-Tag and Streptavidin for Detection and Purification of Recombinant Prot271
  • Chapter 19. Streptavidin-Containing Chimeric Proteins. Design and Production305
  • Chapter 20. Fusions to Maltose-Binding Protein: Control of Folding and Solubility in Protein Purific312
  • Chapter 21. Thioredoxin as a Fusion Partner for Production of Soluble Recombinant Proteins in Escher322
  • Chapter 22. Affinity Purification of Recombinant Proteins Fused to Calmodulin or to Calmodulin-Bindi340
  • Chapter 23. The S ∙ Tag Fusion System for Protein Purification362
  • Chapter 24. Fusions to Self-Splicing Inteins for Protein Purification376
  • Chapter 25. Fusion Proteins Containing Cellulose-Binding Domains418
  • Chapter 26. Biotinylation of Proteins in Vivo and in Vitro Using Small Peptide Tags430
  • Chapter 27. Biotinylation of Proteins in Vivo: A Useful Posttranslational Modification for Protein A440
  • Section V: Hybrid Proteins for Detection and Production of Antigens and Antibodies459
  • Chapter 28. Methods for Generating Multivalent and Bispecific Antibody Fragments461
  • Chapter 29. Design and Use of Phage Display Libraries for the Selection of Antibodies and Enzymes480
  • Chapter 30. Use of an Lpp-OmpA Fusion Vehicle for Bacterial Surface Display506
  • Chapter 31. Identification of Bacterial Class I Accessible Proteins by Disseminated Insertion of Cla516
  • Chapter 32. Bordetella pertussis Adenylate Cyclase Toxin: A Vehicle to Deliver CD8-Positive T-Cell E527
  • Chapter 33. Use of Anthrax Toxin Fusions to Stimulate Immune Responses542
  • Chapter 34. Use of Fusions to Viral Coat Proteins as Antigenic Carriers for Vaccine Development551
  • Author Index571
  • Subject Index601
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822279
  • ISBN-13 9780080496818

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.