Elsevier S & T

Applications of Chimeric Genes and Hybrid Proteins, Part B: Cell Biology and Physiology: Cell Biology and Physiology

Simon, Melvin I.

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Table of contents

Table of Contentsv
Contributors to Volume 327xi
Prefacexvii
Volumes in Seriesxix
Section I: Epitope Tags for Immunodetection1
Chapter 1. Epitope Tagging: General Method for Tracking Recombinant Proteins3
Section II: Markers for Cytology, Analysis of Protein Trafficking, and Lineage Tracing17
Chapter 2. Alkaline Phosphatase Fusions of Ligands or Receptors as in Situ Probes for Staining of Ce19
Chapter 3. Chimeric Molecules Employing Horseradish Peroxidase as Reporter Enzyme for Protein Locali35
Chapter 4. Biochemical Analyses of Trafficking with Horseradish Peroxidase-Tagged Chimeras45
Chapter 5. Visualizing Protein Dynamics in Yeast with Green Fluorescent Protein61
Chapter 6. Kinetic Analysis of Intracellular Trafficking in Single Living Cells with Vesicular Stoma69
Chapter 7. Dual Color Detection of Cyan and Yellow Derivatives of Green Fluorescent Protein Using Co89
Chapter 8. Invertase Fusion Proteins for Analysis of Protein Trafficking in Yeast95
Chapter 9. Introduction of Kex2 Cleavage Sites in Fusion Proteins for Monitoring Localization and Tr107
Chapter 10. Lineage Analysis with Retroviral Vectors118
Chapter 11. Use of Pseudotyped Retroviruses in Zebrafish as Genetic Tags145
Section III: Tools for Analysis of Membrane Proteins163
Chapter 12. A Gene Fusion Method for Assaying Interactions of Protein Transmembrane Segments in Vivo165
Chapter 13. Using SUC2–HIS4C Reporter Domain to Study Topology of Membrane Proteins in Saccharomyc175
Chapter 14. Detecting Interactions between Membrane Proteins in Vivo Using Chimeras190
Chapter 15. Alkaline Phosphatase Fusion Proteins for Molecular Characterization and Cloning of Recep198
Chapter 16. Surface Chimeric Receptors as Tools in Study of Lymphocyte Activation210
Chapter 17. Use of Chimeric Receptor Molecules to Dissect Signal Transduction Mechanisms228
Chapter 18. Fusion Protein Toxins Based on Diphtheria Toxin: Selective Targeting of Growth Factor Re239
Chapter 19. Green Fluorescent Protein-Based Sensors for Detecting Signal Transduction and Monitoring249
Chapter 20. Metabolic Labeling of Glycoproteins with Chemical Tags through Unnatural Sialic Acid Bio260
Section IV: Signals for Addressing Proteins to Specific Subcellular Compartments277
Chapter 21. Using Sorting Signals to Retain Proteins in Endoplasmic Reticulum279
Chapter 22. Directing Proteins to Nucleus by Fusion to Nuclear Localization Signal Tags283
Chapter 23. Identification, Analysis, and Use of Nuclear Export Signals in Saccharomyces cerevisiae297
Chapter 24. Directing Proteins to Mitochondria by Fusion to Mitochondrial Targeting Signals305
Chapter 25. Targeting Proteins to Plasma Membrane and Membrane Microdomains by N-Terminal Myristoyla317
Chapter 26. Analysis of Function and Regulation of Proteins That Mediate Signal Transduction by Use331
Chapter 27. Glycerolphosphoinositide Anchors for Membrane-Tethering Proteins351
Chapter 28. Fusions to Members of Fibroblast Growth Factor Gene Family to Study Nuclear Translocatio369
Section V: Application of Chimeras in Monitoring and Manipulating Cell Physiology383
Chapter 29. Posttranslational Regulation of Proteins by Fusions to Steroid-Binding Domains385
Chapter 30. Tet Repressor-Based System for Regulated Gene Expression in Eukaryotic Cells: Principles401
Chapter 31. Coumermycin-Induced Dimerization of GyrB-Containing Fusion Proteins421
Chapter 32. Use of Glutathione S-Transferase and Break Point Cluster Region Protein as Artificial Di429
Chapter 33. Recombinant Aequorin as Tool for Monitoring Calcium Concentration in Subcellular Compart440
Chapter 34. Recombinant Aequorin as Reporter of Changes in Intracellular Calcium in Mammalian Cells456
Chapter 35. Monitoring Protein Conformations and Interactions by Fluorescence Resonance Energy Trans472
Chapter 36. Studies of Signal Transduction Events Using Chimeras to Green Fluorescent Protein500
Chapter 37. Use of Fusions to Green Fluorescent Protein in the Detection of Apoptosis513
Chapter 38. Synapto-pHluorins: Chimeras between pH-Sensitive Mutants of Green Fluorescent Protein an522
Chapter 39. Studying Organelle Physiology with Fusion Protein-Targeted Avidin and Fluorescent Biotin546
Chapter 40. Fluorescent Labeling of Recombinant Proteins in Living Cells with FlAsH565
Chapter 41. Ubiquitin Fusion Technique and Its Descendants578
Chapter 42. Use of Phosphorylation Site Tags in Proteins594
Author Index615
Subject Index657

Book details

Vendor Elsevier S & T
SKU 9780121822286
ISBN-13 9780080496825
Author Simon, Melvin I.
Category Science
Subject Biochemistry

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

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