Elsevier S & T

Applications of Chimeric Genes and Hybrid Proteins, Part C: Protein-Protein Interactions and Genomics: Protein-Protein Interactions and Genomics

Simon, Melvin I.

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 328ix
  • Prefacexiii
  • Volume in Seriesxv
  • Section I: Applications of Two-Hybrid Methods and Related Techniques for Analysis of Protein–Prote1
  • Chapter 1. High-Throughput Screening for Protein–Protein Interactions Using Two-Hybrid Assay3
  • Chapter 2. LexA-Based Two-Hybrid Systems14
  • Chapter 3. Interaction Mating Methods in Two-Hybrid Systems26
  • Chapter 4. Analysis and Identification of Protein–Protein Interactions Using Protein Recruitment S47
  • Chapter 5. A Bacterial Two-Hybrid System that Exploits a cAMP Signaling Cascade in Escherichia coli59
  • Chapter 6. A Green Fluorescent Protein-Based Reverse Two- Hybrid System: Application to the Characte74
  • Chapter 7. Yeast Three-Hybrid System for Detecting Ligand Receptor Interactions89
  • Chapter 8. Use of a Yeast Three-Hybrid System to Clone Bridging Proteins103
  • Chapter 9. The Yeast Tribrid System: cDNA Expression Cloning of Protein Interactions Dependent on Po111
  • Chapter 10. The Yeast Split-Hybrid System128
  • Section II: Other Approaches Using Chimeras for Identification and Analysis of Interactions of Prote139
  • Chapter 11. Mapping Protein–Protein Interaction Domains Using Ordered Fragment Ladder Far-Western141
  • Chapter 12. Mapping Specificity Determinants for Protein– Protein Association Using Protein Fusion157
  • Chapter 13. Selection of Genetic Agents from Random Peptide Aptamer Expression Libraries171
  • Chapter 14. Detection of Protein–Protein Interactions by Protein Fragment Complementation Strategi208
  • Chapter 15. Monitoring Protein–Protein Interactions in Live Mammalian Cells by B-Galactosidase Co231
  • Chapter 16. Green Fluorescent Protein Chimeras to Probe Protein–Protein Interactions251
  • Chapter 17. Protein Fusions to Coiled-Coil Domains261
  • Chapter 18. Molecular Applications of Fusions to Leucine Zippers282
  • Chapter 19. Using the Yeast Three-Hybrid System to Detect and Analyze RNA–Protein Interactions297
  • Chapter 20. Principles and Applications of a Tat-Based Assay for Analyzing Specific RNA–Protein In322
  • Section III: Phage Display and Its Applications331
  • Chapter 21. Phage Display for Selection of Novel Binding Peptides333
  • Chapter 22. Selectively Infective Phage Technology364
  • Chapter 23. Use of Phage Display and Transition-State Analogs to Select Enzyme Variants with Altered389
  • Chapter 24. Selecting and Evolving Functional Proteins in Vitro by Ribosome Display404
  • Chapter 25. Yeast Surface Display for Directed Evolution of Protein Expression, Affinity, and Stabil430
  • Section IV: Construction of Hybrid Molecules by DNA Shuffiing and Other Methods445
  • Chapter 26. Methods for in Vitro DNA Recombination Random Chimeragenesis447
  • Chapter 27. Random Chimeragenesis by Heteroduplex Recombination456
  • Chapter 28. Use of Chimeras Generated by DNA Shuffiing: Probing Structure–Function Relationships a463
  • Chapter 29. Protein Engineering by Expressed Protein Ligation478
  • Section V: Applications of Fusions in Functional Genomics497
  • Chapter 30. A Systematic and General Proteolytic Method for De.ning Structural and Functional Domain499
  • Chapter 31. High-Throughput Expression of Fusion Proteins515
  • Chapter 32. Rapid Construction of Recombinant DNA by the Univector Plasmid-Fusion System530
  • Chapter 33. High-Throughput Methods for the Large-Scale Analysis of Gene Function by Transposon Tagg550
  • Chapter 34. GATEWAY Recombinational Cloning: Application to the Cloning of Large Numbers of Open Rea575
  • Chapter 35. Gene Trapping Methods for the Identification and Functional Analysis of Cell Surface Pro592
  • Author Index617
  • Subject Index649
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822293
  • ISBN-13 9780080496832
  • Author Simon, Melvin I.
  • Category Science
  • Subject Biochemistry

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.