Elsevier S & T

Bacterial Pathogenesis, Part C: Identification, Regulation and Function of Virulence Factors: Identification, Regulation and Function of Virulence Factors

Clark, Virginia L.

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 358ix
  • Prefacexv
  • Volume in Seriesxvii
  • Section I: Alternatives to Mammalian Model Systems1
  • Chapter 1. Use of Plant and Insect Hosts to Model Bacterial Pathogenesis3
  • Chapter 2. Identification of Host and Pathogen Factors Involved in Virulence Using Caenorhabditis el13
  • Chapter 3. Goldfish as an Animal Model System for Mycobacterial Infection29
  • Section II: Virulence and Essential Gene Identification41
  • Chapter 4. Green Fluorescent Protein as a Marker for Conditional Gene Expression in Bacterial Cells43
  • Chapter 5. Genetic Methods for Deciphering Virulence Determinants of Mycobacterium tuberculosis67
  • Chapter 6. Analysis of Gene Function in Bacterial Pathogens by GAMBIT100
  • Chapter 7. Microbial Gene Expression Elucidated by Selective Capture of Transcribed Sequences (SCOTS108
  • Chapter 8. Identification of Essential Genes in Staphylococcus aureus Using Inducible Antisense RNA123
  • Chapter 9. Transposomes: A System for Identifying Genes Involved in Bacterial Pathogenesis128
  • Chapter 10. Functional Screening of Bacterial Genome for Virulence Genes by Transposon Footprinting141
  • Chapter 11. Use of LexA-Based System to Identify Protein–Protein Interactions in Vivo153
  • Section III: Global Gene Expression: Microarrays and Proteomics163
  • Chapter 12. Borrelia burgdorferi Gene Expression Profiling with Membrane-Based Arrays165
  • Chapter 13. Transcript Profiling of Escherichia coli Using High-Density DNA Microarrays177
  • Chapter 14. Neisseria Microarrays188
  • Chapter 15. Acquisition and Archiving of Information for Bacterial Proteomics: From Sample Preparati207
  • Chapter 16. Proteomic Analysis of pH-Dependent Stress Responses in Escherichia coli and Helicobacter228
  • Chapter 17. Mycobacterial Proteomes242
  • Chapter 18. Proteomic Analysis of Response to Acid in Listeria Monocytogenes256
  • Chapter 19. Proteome Analysis of Chlamydia pneumoniae277
  • Chapter 20. Enrichment and Proteomic Analysis of Low- Abundance Bacterial Proteins288
  • Chapter 21. Immunoproteome of Helicobacter pylori307
  • Section IV: Bacterial Perturbations of Eukaryotic Cell Cycle and Apoptosis317
  • Chapter 22. Helicobacter pylori and Apoptosis319
  • Chapter 23. Modulation of Apoptosis during Infection with Chlamydia334
  • Chapter 24. Measurement of Pore Formation by Contact- Dependent Type III Protein Secretion Systems345
  • Chapter 25. Interaction of Enteropathogenic Escherichia coli with Red Blood Cell Monolayers350
  • Section V: Bacterial Modification or Exploitation of Eukaryotic Signal Transduction357
  • Chapter 26. GAP Activity of Yersinia YopE359
  • Chapter 27. Tyrosine Phosphorylation of Eukaryotic Proteins and Translocated Intimin Receptor by Ent370
  • Section VI: Type III Secretion Systems383
  • Chapter 28. Purification and Detection of Shigella Type III385
  • Chapter 29. Analysis of Salmonella Invasion Protein–Peptidoglycan Interactions393
  • Section VII: Quorum Sensing and Gene Regulation411
  • Chapter 30. RP4-Based Plasmids for Conjugation between Escherichia coli and Members of the Vibrionac413
  • Chapter 31. Role of Autoinducers in Gene Regulation and Virulence of Pseudomonas aeruginosa427
  • Chapter 32. Quorum-Sensing System of Agrobacterium Plasmids: Analysis and Utility452
  • AUTHOR INDEX485
  • SUBJECT INDEX515
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822613
  • ISBN-13 9780080569574
  • Author Clark, Virginia L.
  • Category Science
  • Subject Molecular Biology

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.

Key Features
* Presents alternatives to mammalian model systems
* Discusses virulence and essential gene identification
* Defines global gene expression