Elsevier S & T

Biofilms

Abelson, John N.; Simon, Melvin I.

In stock

No reviews

74.000 KD inc. VAT

License

perpetual

Table of contents

Table of Contentsv
Contributors to Volume 310xi
Prefacexvii
Volumes in Seriesxix
Section I: Molecular Biology of Biofilm Bacteria1
Chapter 1. Reporter Systems for Microscopic Analysis of Microbial Biofilms3
Chapter 2. Molecular Tools for Study of Biofilm Physiology20
Chapter 3. Quorum Sensing Signals in Development of Pseudomonas aeruginosa Biofilms43
Chapter 4. Monitoring Gene Expression in Biofilms56
Chapter 5. In Situ Analysis of Microbial Biofilms by rRNA- Targeted Oligonucleotide Probing79
Chapter 6. Genetic Approaches to Study of Biofilms91
Chapter 7. Identifying in Vivo Expressed Streptococcal Genes in Endocarditis109
Chapter 8. Quorum Sensing, Gene Expression, and Pseudomonas Biofilms117
Section II: Microscopic Methods of Biofilm Formation and Physiology129
Chapter 9. Confocal Laser Scanning Microscopy for Analysis of Microbial Biofilms131
Chapter 10. Lectin-Binding Analysis in Biofilm Systems145
Chapter 11. Spatially Resolved, Quantitative Determination of Luciferase Activity by Photon-Counting152
Chapter 12. Microscopy Flowcells: Perfusion Chambers for Real-Time Study of Biofilms160
Chapter 13. Fluorescent Probes Applied to Physiological and Structural Characterization of Bacterial166
Chapter 14. Deconvolution Fluorescence Microscopy for Observation and Analysis of Membrane Biofilm A178
Chapter 15. Bacterial Biofilms: Strategies for Preparing Glycocalyx for Electron Microscopy194
Section III: Flow and Steady-State Methods205
Chapter 16. Robbins Device in Biofilm Research207
Chapter 17. Controlled Environment Model for Accumulation of Biofilms of Oral Bacteria216
Chapter 18. Laminar Flow Chamber for Continuous Monitoring of Biofilm Formation and Succession224
Chapter 19. Perfused Biofilm Fermenters232
Chapter 20. Laboratory Techniques for Studying Biofilm Growth, Physiology, and Gene Expression in Fl248
Chapter 21. Use of Constant Depth Fermenter in Studies of Biofilms of Oral Bacteria264
Chapter 22. Use of Continuous Flow Techniques in Modeling Dental Plaque Biofilms279
Chapter 23. Steady-State Biofilm: Practical and Theoretical Models296
Chapter 24. Spatial Organization of Oral Bacteria in Biofilms322
Section IV: Biofilms in Archaea333
Chapter 25. Biofilm Formation in Hyperthermophilic Archaea335
Section V: Physical Methods351
Chapter 26. Use of Conductance Measurements for Determination of Enzymatic Degradation of Microbial353
Chapter 27. Evaluation and Quantification of Bacterial Attachment, Microbial Activity, and Biocide E361
Chapter 28. Surface Analyses by X-Ray Photoelectron Spectroscopy in Study of Bioadhesion and Biofilm375
Section VI: Physiology of Biofilm-Associated Microorganisms391
Chapter 29. Evaluating Biofilm Activity in Response to Mass Transfer-Limited Bioavailability of Sorb393
Chapter 30. Bacterial Survival in Biofilms: Probes for Exopolysaccharide and Its Hydrolysis, and Mea403
Chapter 31. Biosurfactants Produced by Lactobacillus426
Chapter 32. Proteome Analysis of Biofilms: Growth of Bacillus subtilis on Solid Medium as Model433
Chapter 33. Physiologic Homeostasis and Stress Responses of Oral Biofilms441
Section VII: Substrate for Biofilm Development461
Chapter 34. Biofouling of Membranes: Membrane Preparation, Characterization, and Analysis of Bacteri463
Chapter 35. Simple Physical Model to Study Formation and Physiology of Biofilms on Urethral Catheter494
Chapter 36. Studying Initial Phase of Biofilm Formation: Molecular Interaction of Host Matrix Protei501
Chapter 37. Studying Biofilm Formation of Mutans Streptococci513
Chapter 38. Models for Studying Initial Adhesion and Surface Growth in Biofilm Formation on Surfaces523
Chapter 39. Recovery and Characterization of Biofilm Bacteria Associated with Medical Devices534
Chapter 40. Primary Adhesion of Pseudomonas aeruginosa to Inanimate Surfaces Including Biomaterials551
Chapter 41. Biofilm and Biofilm-Related Encrustation of Urinary Tract Devices558
Chapter 42. Detection of Prosthetic Joint Biofilm Infection Using Immunological and Molecular Techni566
Chapter 43. In Vitro and in Vivo Models of Bacterial Biofilms577
Section VIII: Antifouling Methods597
Chapter 44. Pseudomonas aeruginosa Biofilm Sensitivity to Biocides: Use of Hydrogen Peroxide as Mode599
Chapter 45. Measuring Antimicrobial Effects on Biofilm Bacteria. From Laboratory to Field608
Chapter 46. Quantitative Assessment of Biocide Control of Biofilms Including Legionella pneumophila629
Chapter 47. Quantifying Effects of Antifouling Paints on Microbial Biofilm Formation637
Chapter 48. Candida Biofilms and Their Susceptibility to Antifungal Agents644
Chapter 49. Enhanced Bacterial Biofilm Control Using Electromagnetic Fields in Combination with Anti656
Author Index671
Subject Index701

Book details

Vendor Elsevier S & T
SKU 9780121822118
ISBN-13 9780080548722
Author Abelson, John N.; Simon, Melvin I.
Category Medical
Subject Pathology

Do you have questions about this book?

Ask an expert!

Volume 310 of Methods in Enzymology is the first volume devoted solely to biofilm research methods. It provides a contemporary source book for virtually any kind of experimental approach involving biofilms. It includes bioengineering, molecular, genetic, microscopic, chemical, continuous culture, and physical methods. This volume will serve as a starting point for future developments.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Instant delivery by email
Your access email arrives within minutes of checkout, with a sign-in link for each book — no shipping, no waiting.

Read on any device
Books open in VitalSource Bookshelf on your phone, tablet, or computer, online or offline. Your library is always available at aafaq.vitalsource.com — just log in with the email you used at checkout.

Lost the email?
Resend it to yourself in seconds from My eBook orders, or email cs@aafaqeducation.com and we'll help.