Biotechnology Annual Review
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Table of contents
- Cover
- Contentsxiii
- Forewordv
- Editorial boardix
- List of contributorsxi
- Chapter 1. Toward a new era in sequencing1
- Sequencing technologies of the next generation1
- Comparison: New fast sequencing technique vs. established Sanger method7
- Applications of the Genome Sequencer 20 system13
- References24
- Chapter 2. Optimizing scaleup yield for protein production: Computationally Optimized DNA Assembly (27
- Introduction28
- Codon pair utilization patterns are highly biased29
- Over-represented codon pairs are translated slowly30
- Over-represented codon pairs occur at protein structural boundaries32
- Misplaced and over-represented codon pairs can inhibit protein expression35
- Hot Rod’ genes produce proteins at high levels37
- Applications of CODA technologies in modern biological drug, diagnostics and vaccine development39
- References40
- Chapter 3. Engineering, expression, purification, and production of recombinant thermolysin43
- Abbreviations43
- Introduction44
- Engineering and expression of recombinant thermolysin46
- Purification of recombinant thermolysin52
- Future perspectives59
- Conclusion60
- Acknowledgments60
- References61
- Chapter 4. Preparation of recombinant vaccines65
- Introduction65
- The different systems to produce recombinant vaccines67
- Vaccine adjuvants, formulation and delivery77
- The use of virus-like particles as foreign antigen carrier systems85
- Conclusion86
- References87
- Chapter 5. Advances in antibody manufacturing using mammalian cells95
- Introduction95
- Upstream considerations98
- Downstream considerations107
- Conclusions111
- References112
- Chapter 6. Pharming and transgenic plants115
- Introduction115
- Plants have a high potential for pharmaceutical protein production116
- Which are the current limitations of the plant expression system?122
- Optimization of plant production platforms for lower proteolytic activity and humanized glycosylatio128
- Emerging plant expression systems for molecular farming132
- Conclusion135
- References135
- Chapter 7. Porous silicon protein microarray technology and ultra-/superhydrophobic states for impro149
- Preface149
- Introduction151
- Porous silicon – a novel 3D substrate for protein microarray technology152
- Effect of porous texture on microarray data quality165
- Superhydrophobic and ultrahydrophobic states on porous silicon172
- MS compatible protein arrays/dual-readout chips180
- Accelerated protein digestion and analyte enrichment in nanovials182
- Superhydrophobic MALDI-TOF MS target anchor chips185
- Conclusions188
- Acknowledgments189
- References189
- Chapter 8. Production of plasmid DNA for pharmaceutical use201
- Introduction201
- Plasmid production202
- Conclusions218
- References218
- Chapter 9. Potentials of phenolic molecules of natural origin and their derivatives as anti-HIV agen223
- Abbreviations used223
- Introduction225
- The AIDS and its therapy225
- Plant materials and extracts against HIV-1: Few examples228
- In silico approaches for the discovery of anti-HIV molecules from natural products230
- Docking and virtual screening230
- Caffeic acid derivatives from food supplements against HIV231
- The HIV-1 life cycle and the mechanism of action of anti-HIV compounds from medicinal plants232
- Phenolics – a promising class of molecules against HIV234
- Some biological activities of phenolics235
- Phenolics acting on viral targets237
- Chemogenomics in anti-HIV drug discovery252
- Conclusions, perspectives and recommendations255
- Acknowledgments256
- References256
- Chapter 10. Ganoderma lucidum and its pharmaceutically active compounds265
- Introduction265
- G. lucidum cultivation methods267
- Cultivation of fruit bodies on natural wood logs268
- Cultivation of fruit bodies on sawdust substrates270
- Cultivation of G. lucidum mycelia in a bioreactor272
- Main pharmacologically active compounds in G. lucidum276
- Triterpenoids from Ganoderma mushrooms276
- Polysaccharides from Ganoderma fruit bodies and mycelia283
- Regulation and protection of cells288
- Peptidoglycanes and proteins288
- Other compounds289
- Formulations, market products and clinical trials290
- Conclusion292
- References292
- Chapter 11. Citric acid production303
- Introduction303
- Uses and occurrence304
- Strains for citric acid production305
- Biochemistry306
- Substrates314
- Production processes318
- Product recovery324
- Economic aspects329
- Citric acid World production330
- References334
- Chapter 12. Using nanotechnology to improve the characteristics of antineoplastic drugs: Improved ch345
- Introduction345
- Paclitaxel346
- nab-Paclitaxel347
- Nonclinical studies with nab-paclitaxel348
- Clinical studies with nab-paclitaxel348
- Discussion354
- References354
- Index of authors359
- Keyword index361
Biotechnology is a diverse, complex and rapidly evolving field. Students and experienced researchers alike face the challenges of staying on top of developments in their field of specialty and maintaining a broader overview of the field as a whole. Volumes containing competent reviews on a diverse range of topics in the field fulfill the dual role of broadening and updating biotechnologists’ knowledge. The current volume is an excellent example of such a book. The topics covered range from classical issues in biotechnology - such as, recent advances in all-protein chromophore technology- to topics that are focused on sequencing and recombinant vaccines. The information presented in this book will therefore will be of great value to both experienced biotechnologists and biotechnologists in training.
* Includes over 200 figures with more than 60 tables
* Discusses valuable information on pharming & transgenic plants
* Provides detailed information on the production of plasmid DNA for pharmaceutical use
* Includes over 200 figures with more than 60 tables
* Discusses valuable information on pharming & transgenic plants
* Provides detailed information on the production of plasmid DNA for pharmaceutical use