Elsevier S & T

Carbon-13 NMR Spectroscopy of Biological Systems

Beckmann, Nicolau

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Table of contents
  • Contentsvii
  • Contributorsxv
  • Forewordxvii
  • Prefacexix
  • Chapter 1. Introduction1
  • 1.1 The Beginning2
  • 1.2 Essential Developments for 13C NMR2
  • 1.3 13C NMR in the Solid State: Cross-Polarization and Magic-Angle Spinning3
  • 1.4 Heteronuclear Polarization Transfer4
  • References4
  • Chapter 2. Methodology and Applications of Heteronuclear and Multidimensional 13C NMR to the Elucida7
  • 2.1 Introduction7
  • 2.2 General Considerations8
  • 2.3 Heteronuclear Coherence Transfer13
  • 2.4 Multidimensional Correlation Experiments19
  • 2.5 Determination of l3C Relaxation Times51
  • 2.6 Heteronuclear Coupling Constants53
  • 2.7 Concluding Remarks61
  • References61
  • Chapter 3 . Measurement of Internuclear Distances in Biological Solids by Magic-Angle Spinning 13C N65
  • 3.1 Introduction65
  • 3.2 Measuring Distances between Isolated Heteronuclear Spin Pairs by MAS NMR69
  • 3.3 13C–13C Distances by MAS NMR95
  • 3.4 Concluding Remarks112
  • References113
  • Chapter 4. 13C NMR Studies of the Interactions of Fatty Acids with Phospholipid Bilayers, Plasma Lip117
  • 4.1 Introduction117
  • 4.2 Properties of Fatty Acids118
  • 4.3 Interactions of Fatty Acids with Serum Albumin119
  • 4.4 Binding of Medium-Chain Fatty Acids to Human Serum Albumin132
  • 4.5 Binding of Fatty Acids to Human Serum Lipoproteins134
  • 4.6 Binding of Fatty Acids to Cytosolic Fatty Acid Binding Proteins138
  • 4.7 Binding of Fatty Acids to Model Membranes139
  • 4.8 Exchange of Fatty Acids between Albumin and Model Membranes143
  • 4.9 Exchange of Fatty Acids among Proteins, Model Membranes, and Lipoproteins151
  • 4.10 Concluding Remarks154
  • References155
  • Chapter 5. Application of 13C NMR Spectroscopy to Metabolic Studies on Animals159
  • 5.1 Introduction159
  • 5.2 13C MRS and the Concepts of 13C Labeling161
  • 5.3 13C MRS, 13C Labeling, and Models of Intermediary Metabolism168
  • 5.4 Experimental Considerations176
  • 5.5 Metabolism in the Liver186
  • 5.6 Metabolism in the Heart214
  • 5.7 Metabolism in the Brain226
  • 5.8 Metabolism in the Ocular Lens241
  • 5.9 Metabolism in the Kidneys244
  • 5.10 Metabolism in the Skeletal Muscles250
  • 5.11 Metabolism in Parasites251
  • 5.12 Metabolism in Tumors257
  • 5.13 Concluding Remarks260
  • References261
  • Chapter 6. 13C Magnetic Resonance Spectroscopy as a Noninvasive Tool for Metabolic Studies on Humans269
  • 6.1 Introduction269
  • 6.2 Coils271
  • 6.3 Decoupling272
  • 6.4 Schemes for the Acquisition and Enhancement of 13C Signals and for 13C Editing273
  • 6.5 Spatially Localized 13C Spectroscopy276
  • 6.6 Absolute Quantification of Metabolites288
  • 6.7 In Vivo Metabolic Studies with 13C MRS289
  • 6.8 Noninvasive Determination of the Degree of Unsaturation of Fatty Acids from Adipose Tissue304
  • 6.9 Body Fluids and Isolated Tissues305
  • 6.10 Diseased States307
  • 6.11 Concluding Remarks316
  • References318
  • Index323
Book details
  • Vendor Elsevier S & T
  • SKU 9780120843701
  • ISBN-13 9780080528557
  • Author Beckmann, Nicolau
  • Category Science
  • Subject Biotechnology

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Description eBook delivery & access Reviews
This book is intended to provide an in-depth understanding of 13C NMR as a tool in biological research. 13C NMR has provided unique information concerning complex biological systems, from proteins and nucleic acids to animals and humans. The subjects addressed include multidimensional heteronuclear techniques for structural studies of molecules in the liquid and solid states, the investigation of interactions in model membranes, the elucidation of metabolic pathwaysin vitro and in vivo on animals, and noninvasive metabolic studies performed on humans. The book is a unique mix of NMR methods and biological applications which makes it a convenient reference for those interested in research in this interdisciplinary area of physics, chemistry, biology, and medicine.

Key Features
* An interdisciplinary text with emphasis on both 13C NMR methodology and the relevant biological and biomedical issues
* State-of-the-art 13C NMR techniques are described; Whenever possible, their advantages over other approaches are emphasized
* The chapters constitute comprehensive reviews and are written by acknowledged experts in their fields
* Chapters are written in a clear style, and include a large number of illustrations and comprehensive references