Elsevier S & T

Chromatin and Chromatin Remodeling Enzymes, Part B

Wu, Carl; Allis, C. David

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 376ix
  • Prefacexv
  • Volumes in Seriesxvii
  • Section I: Biophysics, Structural Biology, and Enzymology of Chromatin Proteins1
  • Chapter 1. Fluorescence Anisotropy Assays for Analysis of ISWI-DNA and ISWI-Nucleosome Interactions3
  • Chapter 2. Biophysical Analysis of Specific Genomic Loci Assembled as Chromatin In Vivo17
  • Chapter 3. Visualization and 3D Structure Determination of Defined Sequence Chromatin and Chromatin29
  • Chapter 4. Electron Microscopic Analysis of the RSC Chromatin Remodeling Complex48
  • Chapter 5. Use of Optical Trapping Techniques to Study Single-Nucleosome Dynamics62
  • Chapter 6. Single-Molecule Analysis of Chromatin73
  • Chapter 7. Biochemical and Structural Characterization of Recombinant Histone Acetyltransferase Prot106
  • Chapter 8. Use of Nuclear Magnetic Resonance Spectroscopy to Study Structure-Function of Bromodomain119
  • Chapter 9. Assays for the Determination of Structure and Dynamics of the Interaction of the Chromodo131
  • Chapter 10. Expression, Purification, and Biophysical Studies of Chromodomain Proteins148
  • Chapter 11. Quantitative Assays for Characterization of the Sir2 Family of NAD+-Dependent Deacetylas171
  • Chapter 12. Selective HAT Inhibitors as Mechanistic Tools for Protein Acetylation188
  • Chapter 13. Histone Deacetylase Inhibitors: Assays to Assess Effectiveness In Vitro and In Vivo199
  • Section II: Immunochemical Assays of Chromatin Functions207
  • Chapter 14. Immunochemical Analysis of Chromatin209
  • Chapter 15. Generation and Characterization of Antibodies Directed Against Di-Modified Histones, and221
  • Chapter 16. Generation and Characterization of Methyl-Lysine Histone Antibodies234
  • Chapter 17. Tips in Analyzing Antibodies Directed Against Specific Histone Tail Modifications255
  • Chapter 18. Histone Methylation: Recognizing the Methyl Mark269
  • Chapter 19. Analysis of Genome-Wide Histone Acetylation State and Enzyme Binding Using DNA Microarra289
  • Chapter 20. Use of Chromatin Immunoprecipitation Assays in Genome-Wide Location Analysis of Mammalia304
  • Chapter 21. High-Throughput Screening of Chromatin Immunoprecipitates Using CpG-Island Microarrays315
  • Chapter 22. Chromatin Immunoprecipitation in the Analysis of Large Chromatin Domains Across Murine A334
  • Chapter 23. The Use of Chromatin Immunoprecipitation Assays in Genome-Wide Analyses of Histone Modif349
  • Chapter 24. Sequential Chromatin Immunoprecipitation from Animal Tissues361
  • Chapter 25. Immunofluorescent Staining of Polytene Chromosomes: Exploiting Genetic Tools372
  • Chapter 26. Indirect Immunofluorescent Labeling of Drosophila Polytene Chromosomes: Visualizing Prot393
  • Chapter 27. X-Chromosome Inactivation in Mouse Embryonic Stem Cells: Analysis of Histone Modificatio405
  • Chapter 28. X Inactivation in Mouse ES Cells: Histone Modifications and FISH419
  • Author Index429
  • Subject Index449
Book details
  • Vendor Elsevier S & T
  • SKU 9780121827809
  • ISBN-13 9780080497143
  • Author Wu, Carl; Allis, C. David
  • Category Science
  • Subject Research & Methodology

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DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodelling enzymes play an important role in gene transcription.