Elsevier S & T

Chromatin and Chromatin Remodeling Enzymes Part C

Wu, Carl; Allis, C. David

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 377ix
  • Prefacexv
  • Volumes in Seriesxvii
  • Section I: Genetic Assays of Chromatin Modification and Remodeling1
  • Chapter 1. Functional Analyses of Chromatin Modifications in Yeast3
  • Chapter 2. Genetic Analysis of Chromatin Remodeling Using Budding Yeast as a Model55
  • Chapter 3. Introduction to Trx-G and Pc-G Genes61
  • Chapter 4. Genetic and Cytological Analysis of Drosophila Chromatin-Remodeling Factors70
  • Chapter 5. Genetic Analysis of H1 Linker Histone Subtypes and Their Functions in Mice85
  • Section II: Histone Modifying Enzymes109
  • Chapter 6. The Use of Mass Spectrometry for the Analysis of Histone Modifications111
  • Chapter 7. Histone Modification Patterns During Gene Activation130
  • Chapter 8. Identification and Analysis of Native HAT Complexes154
  • Chapter 9. Histone Deacetylases: Purification of the Enzymes, Substrates, and Assay Conditions167
  • Chapter 10. Measurement of Mammalian Histone Deacetylase Activity180
  • Chapter 11. Analysis of Histone Phosphorylation: Coupling Intracellular Signaling to Chromatin Remod197
  • Chapter 12. Purification of Histone Methyltransferases from HeLa Cells213
  • Chapter 13. Global Proteomic Analysis of S. cerevisiae (GPS) to Identify Proteins Required for Histo227
  • Chapter 14. Purification of Sir2 Proteins from Yeast234
  • Chapter 15. Purification and Biochemical Properties of the Drosophila TAC1 Complex255
  • Chapter 16. Isolation and Characterization of CHRASCH, a Polycomb-Containing Silencing Complex267
  • Chapter 17. Biochemical Analysis of Mammalian Polycomb Group Protein Complexes and the Identificatio282
  • Section III: ATP-Dependent Chromatin Remodeling Enzymes297
  • Chapter 18. Purification and Functional Analysis of the Mammalian SWI/SNF-Family of Chromatin- Remod299
  • Chapter 19. Isolation and Assay of the RSC Chromatin- Remodeling Complex from Saccharomyces cerevisi316
  • Chapter 20. DNA Translocation and Nucleosome Remodeling Assays by the RSC Chromatin Remodeling Compl322
  • Chapter 21. A Nucleosome Sliding Assay for Chromatin Remodeling Factors344
  • Chapter 22. Methods for Analysis of Nucleosome Sliding by Drosophila NURF353
  • Chapter 23. Methods for Preparation and Assays for Xenopus ISWI Complexes364
  • Chapter 24. Functional Analysis of ISWI Complexes in Mammalian Cells376
  • Chapter 25. Preparation and Assays for Mammalian ISWI Complexes389
  • Chapter 26. Preparation and Analysis of the INO80 Complex401
  • Chapter 27. Assay of Z-DNA Induction by Chromatin Remodeling Factors412
  • Chapter 28. Immuno-Depletion and Purification Strategies to Study Chromatin-Remodeling Factors In Vi421
  • Section IV: Transcription and Other Transactions on Chromatin Templates443
  • Chapter 29. Transcription Through the Nucleosome by mRNA-producing RNA Polymerases445
  • Chapter 30. Reconstitution and Transcriptional Analysis of Chromatin In Vitro460
  • Chapter 31. Techniques Used to Study Transcription on Chromatin Templates474
  • Chapter 32. Analysis of DNA Repair on Nucleosome Templates499
  • Author Index509
  • Subject Index535
Book details
  • Vendor Elsevier S & T
  • SKU 9780121827816
  • ISBN-13 9780080497150
  • Author Wu, Carl; Allis, C. David
  • Category Medical
  • Subject Biochemistry

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DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the chromatin remodelling enzymes play an important role in gene transcription.

*Genetic assays of chromatin modification and remodeling
*Histone modifying enzymes
*ATP-dependent chromatin remodeling enzymes