Elsevier S & T

Confocal Microscopy

Abelson, John N.; Simon, Melvin I.

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Table of contents
  • Table of Contentsv
  • Contributors to Volume307ix
  • Prefacexiii
  • Volumes in Seriesxv
  • Section I: Theory and Practical Considerations1
  • Chapter 1. Theoretical Basis of Confocal Microscopy3
  • Chapter 2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope20
  • Section II: General Techniques27
  • Chapter 3. Equipment for Mass Storage and Processing of Data29
  • Chapter 4. Antifading Agents for Confocal Fluorescence Microscopy55
  • Chapter 5. Mounting Techniques for Confocal Microscopy79
  • Chapter 6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy84
  • Chapter 7. Cytotomography108
  • Chapter 8. Use of Confocal Microscopy to Investigate Cell Structure and Function119
  • Chapter 9. Combining Laser Scanning Confocal Microscopy and Electron Microscopy135
  • Chapter 10. Construction of Line-Scan Confocal Microscope for Physiological Recording152
  • Section III: Measurement of Subcellular Relations and Volume Determinations171
  • Chapter 11. Three-Dimensional Visualization of Cytoskeleton by Confocal Laser Scanning Microscopy173
  • Chapter 12. Chromosome Spread for Confocal Microscopy190
  • Chapter 13. Visualization of Nuclear Pore Complex and Its Distribution by Confocal Laser Scanning Mi207
  • Chapter 14. Measurement of Tissue Thickness Using Confocal Microscopy230
  • Chapter 15. Measurements of Vascular Remodeling by Confocal Microscopy246
  • Chapter 16. Cell Volume Measurements by Fluorescence Confocal Microscopy: Theoretical and Practical274
  • Chapter 17. Volume Measurements in Confocal Microscopy296
  • Section IV: Imaging of Processes317
  • Chapter 18. Quantitation of Phagocytosis by Confocal Microscopy319
  • Chapter 19. Measurement of Secretion in Confocal Microscopy328
  • Chapter 20. Receptor–Ligand Internalization340
  • Chapter 21. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy351
  • Chapter 22. Localization of Proteases and Peptide Receptors by Confocal Microscopy368
  • Chapter 23. Observation of Microcirculatory Kinetics by Real-Time Confocal Laser Scanning Microscopy394
  • Section V: Imaging of Ions423
  • Chapter 24. Confocal Imaging Analysis of Intracellular Ions in Mixed Cellular Systems or in Situ Usi425
  • Chapter 25. Confocal Ca2+ Imaging of Organelles, Cells, Tissues, and Organs441
  • Chapter 26. Using Confocal Microscopy and Fluorescent Indicator, 6-Methoxy-N-ethylquinolinium Iodide469
  • Section VI: Imaging of Specialized Tissues483
  • Chapter 27. Measurement of Mineral Gain and Loss on Dental Enamel and Dentin Using Confocal Microsco485
  • Chapter 28. Characterization of Ocular Cellular and Extracellular Structures Using Confocal Microsco496
  • Chapter 29. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells513
  • Chapter 30. Video-Rate, Scanning Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dime536
  • Chapter 31. In Vivo Imaging of Mammalian CNS Neurons with ICON563
  • Section VII: Imaging Viruses and Fungi571
  • Chapter 32. Identification of Viral Infection by Confocal Microscopy573
  • Chapter 33. Using Confocal Microscopy to Study Virus Binding and Entry into Cells591
  • Chapter 34. Fluorescent Labels, Confocal Microscopy, and Quantitative Image Analysis in Study of Fun607
  • Author Index625
  • Subject Index647
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822088
  • ISBN-13 9780080496658
  • Author Abelson, John N.; Simon, Melvin I.
  • Category Science
  • Subject Biochemistry

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This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.