Elsevier S & T

DNA Arrays in Neurobiology

Miles, Michael

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Table of contents
  • Contentsv
  • Contributorsix
  • Introductionxi
  • Chapter 1. Microarray Platforms: Introduction and Application to Neurobiology1
  • I. Introduction1
  • II. Experimental Flow3
  • III. Microarray Design6
  • IV. Slides and Probes7
  • V. Sample Preparation and Labeling11
  • VI. Data Analysis12
  • VII. Data Interpretation13
  • VIII. Confirmation of Microarray Results14
  • IX. Conclusion15
  • X. Online Resources16
  • References18
  • Chapter 2. Experimental Design and Low-Level Analysis of Microarray Data25
  • I. Introduction25
  • II. Design of Experiments26
  • III. Sample Size Considerations32
  • IV. Normalization38
  • V. Expression Summaries for GeneChip Data45
  • VI. Quality Assessment47
  • VII. Detection of Absolute Gene Expression54
  • References57
  • Chapter 3. Brain Gene Expression: Genomics and Genetics59
  • I. Introduction60
  • II. Transcript Abundance as a Complex Trait62
  • III. Genetic Dissection of Transcription Regulation66
  • IV. How Many QTLs Are There? Statistical Control in Two Dimensions77
  • V. Probe Versus Probe Set–Level Phenotypes79
  • VI. Transcription Regulatory Networks83
  • VII. Functional Correlates of Brain Transcriptional Activity85
  • VIII. Conclusions: Building a Model of Brain Function from Multipoint, Multi-tissue Gene Expression,90
  • References92
  • Chapter 4. DNA Microarrays and Animal Models of Learning and Memory97
  • I. Introduction97
  • II. DNA Microarray Technology98
  • III. Use of DNA Microarrays for Studying Learning and Memory104
  • IV. Conclusion126
  • References126
  • Chapter 5. Microarray Analysis of Human Nervous System Gene Expression in Neurological Disease135
  • I. Introduction135
  • II. Disease Pathophysiology136
  • III. Microarray-Based Disease Classification137
  • IV. Human Neurological Diseases Studied by Microarray Technology141
  • V. Conclusion145
  • References146
  • Chapter 6. DNA Microarray Analysis of Postmortem Brain Tissue153
  • I. Introduction153
  • II. Challenges in Studies of the Postmortem Human Brain154
  • III. Microarray Analysis of Human Brain Disorders160
  • IV. Where Do We Go from Here?171
  • References173
  • Index183
  • Contents of Recent Volumes191
Book details
  • Vendor Elsevier S & T
  • SKU 9780123668615
  • ISBN-13 9780080495514
  • Author Miles, Michael
  • Category Medical
  • Subject Genetics

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DNA array technology is a technique for studying gene expression by comparing samples of different genes. The result is an enormous amount of data that must be carefully analyzed in order for it to be useful and meaningful. This book examines both data analysis and techniques for ensuring optimal experimental conditions. The array approach has applications in a number of model systems, including development, learning and drug abuse. In addition, the technique has applications in a number of neurological disorders such as Alzheimer's disease, schizophrenia, multiple sclerosis, and neurological cancers.