Elsevier S & T

Drug-Nucleic Acid Interactions

Chaires, Jonathan B.

In stock

No reviews

77.750 KD inc. VAT

License

perpetual

Table of contents

Table of Contentsv
Contributors to Volume 340ix
Prefacexiii
Volume in Seriesxv
Section I: Biophysical Approaches1
Chapter 1. Analysis and Interpretation of Ligand–DNA Binding Isotherms3
Chapter 2. Surface Plasmon Resonance Biosensor Analysis of RNA–Small Molecule Interactions22
Chapter 3. Simultaneous Measurement of Binding Constants and Unwinding Angles by Gel Electrophoresis51
Chapter 4. Linear and Circular Dichroism of Drug–Nucleic Acid Complexes68
Chapter 5. Rapid Screening of Structurally Selective Ligand Binding to Nucleic Acids99
Chapter 6. Calorimetric Techniques in the Study of High-Order DNA–Drug Interactions109
Chapter 7. Volume Changes Accompanying Interaction of Ligands with Nucleic Acids149
Chapter 8. Calculating Sequence-Dependent Melting Stability of Duplex DNA Oligomers and Multiplex Se165
Chapter 9. Thermal Denaturation as Tool to Study DNA–Ligand Interactions193
Chapter 10. Kinetics of Binding of Hoechst Dyes to DNA Studied by Stopped-Flow Fluorescence Techniqu212
Chapter 11. Scanning Force Microscopy of Nucleic Acid Complexes234
Chapter 12. Nuclear Magnetic Resonance Studies of Drug–DNA Complexes in Solution252
Chapter 13. X-Ray Crystallography of DNA–Drug Complexes282
Chapter 14. Molecular Modeling of Drug–DNA Complexes: An Update290
Section II: Chemical and Molecular Biological Approaches327
Chapter 15. Peptide Nucleic Acid Targeting of Double-Stranded DNA329
Chapter 16. Drug Interaction with Triple-Helical Nucleic Acids340
Chapter 17. Measurement of Covalent Drug–DNA Interactions at the Nucleotide Level in Cells at Phar358
Chapter 18. Targeting DNA through Covalent Interactions of Reversible Binding Drugs377
Chapter 19. Chemical Cross-Linking of Drugs to DNA396
Chapter 20. High-Resolution Footprinting Studies of Drug–DNA Complexes Using Chemical and Enzymati412
Chapter 21. Drug–RNA Footprinting431
Chapter 22. Footprinting Methods for Analysis of Pyrrole–Imidazole Polyamide/DNA Complexes450
Chapter 23. High-Resolution Transcription Assay for Probing Drug–DNA Interactions at Individual Dr466
Chapter 24. Use of DNA Molecules Substituted with Unnatural Nucleotides to Probe Speci.c Drug–DNA485
Chapter 25. Drug Interactions with Nucleosomes and Chromatin503
Chapter 26. Locating Cobalt-Binding Sites on DNA Using Restriction Endonucleases519
Chapter 27 Exploiting Anthracycline Scaffold for Designing DNA-Targeting Agents529
Chapter 28. Design, Synthesis, and Characterization of Polyintercalating Ligands556
Section III: Enzymology and Biological Approaches571
Chapter 29. Targeting Telomeres and Telomerase573
Chapter 30. Rapid, High-Throughput Engineering of Sequence-Specific Zinc Finger DNA-Binding Proteins593
Chapter 31. DNA Relaxation and Cleavage Assays to Study Topoisomerase I Inhibitors610
Chapter 32. In Vitro Human Immunodeficiency Virus Type 1 Integrase Assays624
Chapter 33. Use of Xenopus Egg Extracts to Study Effects of DNA-Binding Drugs on Chromatin Assembly,634
Author Index655
Subject Index689

Book details

Vendor Elsevier S & T
SKU 9780121822415
ISBN-13 9780080496900
Author Chaires, Jonathan B.
Category Science
Subject Research & Methodology

Do you have questions about this book?

Ask an expert!

This volume consolidates the key methods for studying ligand-nucleic acid interactions into a convenient source. Techniques that are examined range from biophysical and chemical approaches to methods rooted in molecular and cell biology.

Instant delivery by email
Your access email arrives within minutes of checkout, with a sign-in link for each book — no shipping, no waiting.

Read on any device
Books open in VitalSource Bookshelf on your phone, tablet, or computer, online or offline. Your library is always available at aafaq.vitalsource.com — just log in with the email you used at checkout.

Lost the email?
Resend it to yourself in seconds from My eBook orders, or email cs@aafaqeducation.com and we'll help.