Energetics of Biological Macromolecules, Part E

Holt, Jo M.

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Table of contents
  • Table of Contentsvii
  • Contributors to Volume 380ix
  • Prefacexi
  • Volumes in Seriesxiii
  • Section I: Allosteric Enzymes and Receptors1
  • Chapter 1. Contributions to the Catalytic Efficiency of Enzymes, and the Binding of Ligands to Recep3
  • Chapter 2. Structural Interpretation of pH and Salt-Dependent Processes in Proteins with Computation20
  • Chapter 3. Electrostatic Basis for Bioenergetics52
  • Chapter 4. Local and Global Control Mechanisms in Allosteric Threonine Deaminase85
  • Chapter 5. Methods for Analyzing Cooperativity in Phosphoglycerate Dehydrogenase106
  • Chapter 6. Fluorescent Probes Applied to Catalytic Cooperativity in ATP Synthase132
  • Chapter 7. Measurement of Energetics of Conformational Change in Cobalamin-Dependent Methionine Synt152
  • Chapter 8. Spectroscopic and Kinetic Methods for Ligand–Protein Interactions of Glutamate Receptor170
  • Chapter 9. Quantitative Analysis and Interpretation of Allosteric Behavior187
  • Section II: Cooperativity in Protein Folding and Assembly205
  • Chapter 10. The Immobilized Template Assay for Measuring Cooperativity in Eukaryotic Transcription C207
  • Chapter 11. Characterization of the Cargo Attachment Complex of Cytoplasmic Dynein Using NMR and Mas219
  • Chapter 12. Circular Dichroism of Protein-Folding Intermediates242
  • Chapter 13. Amide Hydrogen Exchange/Mass Spectrometry Applied to Cooperative Protein Folding: Equili285
  • Chapter 14. Kinetic and Spectroscopic Analysis of Early Events in Protein Folding308
  • Chapter 15. Hydrogen-Exchange Strategies Applied to Energetics of Intermediate Processes in Protein328
  • Chapter 16. Cooperativity Principles in Protein Folding350
  • Chapter 17. Native State Hydrogen-Exchange Analysis of Protein Folding and Protein Motional Domains379
  • Chapter 18. The Preparation of 19F-Labeled Proteins for NMR Studies400
  • Author Index417
  • Subject Index435
Book details
  • Vendor Elsevier S & T
  • SKU 9780121827847
  • ISBN-13 9780080497181
  • Author Holt, Jo M.
  • Category Science
  • Subject Molecular Biology

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This volume focuses on methods related to allosteric enzymes and receptors, including fluorescent proves, spectroscopic methods and quantitative analysis as well as on cooperativity in protein folding. NMR and mass spectrometry methods are discussed.

*Allosteric Enzymes and Receptors
*Cooperativity in Protein Folding and Assembly