Energy Coupling and Molecular Motors
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Table of contents
- THE ENZYMESiii
- Copyright Pageiv
- Contentsvii
- Prefacexi
- Chapter 1. Muscle Contraction1
- I. Introduction2
- II. Sarcomere Structure3
- III. Distribution of Myosin Superfamily Members and Contractile Proteins7
- IV. Myosin Structure8
- V. Working Hypothesis12
- VI. Actomyosin ATPase Cycle in Solution15
- VII. Comparison of ATPase Kinetics Between a Protein Suspension and the Sarcomeric Filament Lattice20
- VIII. Myofibrillar ATPase21
- IX. Muscle Fiber Mechanics and Energetics22
- X. Biochemical Rate Constants in Muscle Fibers28
- XI. Structural Changes Leading to Force Generation and Filament Sliding33
- XII. Why Does Myosin Have Two Heads?42
- XIII. Summary, Uncertainties and Future Directions43
- References45
- Chapter 2. Mechanics of Unconventional Myosins55
- I. Introduction55
- II. Single-Molecule Analysis Revealed a Unitary Small Step in Motion as Myosin Interacts with Actin57
- III. Molecular Genetic Approaches have Indicated Roles of Various Domains and Specific Residues of t59
- IV. The Unconventional Myosins V and VI are Adapted for Cellular Transport Roles61
- V. Requirements of Processive Motors64
- VI. Conclusions and Perspectives82
- References83
- Chapter 3. Motor Proteins of the Kinesin Superfamily87
- I. Introduction88
- II. Structure90
- III. Characterization of Motility101
- IV. ATPase Mechanism107
- V. MT Decoration117
- VI. Generation of Motility120
- VII. Regulation and Cargo Binding133
- VIII. Perspectives136
- References137
- Chapter 4. The Bacterial Rotary Motor143
- I. Introduction144
- II. Bacterial Behavior145
- III. The Flagellar Motor151
- IV. Future Work184
- References185
- Chapter 5. The ATP Synthase: Parts and Properties of a Rotary Motor203
- I. Overview204
- II. Conservation of General Structure and Function in FOF1205
- III. The Binding-change Mechanism for ATP Synthesis and Hydrolysis208
- IV. F1’s Structural Compatibility with a Cooperative, Rotary Mechanism210
- V. Demonstration and Analysis of Subunit Rotation in F1 and in FOF1216
- VI. Further Characteristics of FO and F1 Subunits as Components of the Rotor or Stator235
- VII. Remaining Puzzles for Rotational Catalysis262
- References265
- Chapter 6. Bacteriophage T7 Gene 4 Protein: A Hexameric DNA Helicase277
- I. Introduction277
- II. Isolation and Characterization281
- III. Structural and Biochemical Properties283
- IV. Models for Energy Transduction290
- V. Future Directions298
- References299
- Chapter 7. DNA Helicases, Motors that Move Along Nucleic Acids: Lessons from the SF1 Helicase Superf303
- I. Introduction304
- II. Phenomenological Features of DNA Unwinding307
- III. Structural Features of SF1 DNA Helicases309
- IV. Protein Oligomerization320
- V. DNA Binding by E. coli Rep323
- VI. Mechanisms of Nucleotide Binding and ATP Hydrolysis by E. coli Rep329
- VII. Single-Stranded DNA Translocation by Monomers of SF1 Helicases333
- VIII. Presteady-State, Single-Turnover DNA Unwinding Studies336
- IX. DNA Unwinding by E. coli Rep and UvrD Helicases339
- X. Helicase Activity of SF1 Monomers348
- XI. E. coli RecBCD Helicase350
- XII. Proposed Mechanisms for DNA Unwinding and Translocation by SF1 Helicases354
- XIII. Summary362
- References364
- Chapter 8. Type II DNA Topoisomerases: Coupling Directional DNA Transport to ATP Hydrolysis371
- I. Introduction372
- II. Basic Biochemical and Structural Information about Type II Topoisomerases374
- III. The Mechanism of Strand Passage380
- IV. Concluding Thoughts393
- References394
- Chapter 9. The Role of ATP in Directing Chaperonin-Mediated Polypeptide Folding399
- I. The Work Carried out by Chaperonins400
- II. ATP Action in Driving Chaperonin-Assisted Protein Folding – Structural States and the Overall402
- III. Mechanistic Studies of the Nucleotide Cycle411
- IV. Polypeptide and the Nucleotide Cycle416
- V. Cooperativity and Allostery421
- References430
- Author Index435
- Subject Index469
- Color Plate SectionColor Plate-1
This volume examines a number of different molecular motors that utilize ATP. The molecular machines to be discussed include ATP synthase, myosin, kinesin, DNA helicases, DNA topoisomerases, chaperones and bacterial rotory motors. The discussion of these various molecular motors is rarely undertaken in one volume and will serve as a great resource for scientists studying structure and function of multiprotein complexes as well as those working on energy coupling mechanisms. The areas of research presented in this volume do not normally overlap, and yet they share common mechanisms.
This volume examines a number of different molecular motors that utilize ATP. The molecular machines to be discussed include ATP synthase, myosin, kinesin, DNA helicases, DNA topoisomerases, chaperones and bacterial rotory motors. The discussion of these various molecular motors is rarely undertaken in one volume and will serve as a great resource for scientists studying structure and function of multiprotein complexes as well as those working on energy coupling mechanisms. The areas of research presented in this volume do not normally overlap, and yet they share common mechanisms.
This volume examines a number of different molecular motors that utilize ATP. The molecular machines to be discussed include ATP synthase, myosin, kinesin, DNA helicases, DNA topoisomerases, chaperones and bacterial rotory motors. The discussion of these various molecular motors is rarely undertaken in one volume and will serve as a great resource for scientists studying structure and function of multiprotein complexes as well as those working on energy coupling mechanisms. The areas of research presented in this volume do not normally overlap, and yet they share common mechanisms.