Elsevier S & T

Flavonoids and Other Polyphenols: Methods in Enzymology, Vol. 335

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 335ix
  • Prefacexiii
  • Volume in Seriesxv
  • Section I: Sources, Characterization, and Analytical Methods1
  • Chapter 1. Overview of Methods for Analysis and Identification of Flavonoids3
  • Chapter 2. Analysis of Complex Mixtures of Flavonoids and Polyphenols by High-Performance Liquid Chr15
  • Chapter 3. Analysis of Flavonoids in Medicinal Plants26
  • Chapter 4. High-Performance Liquid Chromatography/Mass Spectrometry Analysis of Proanthocyanidins in46
  • Chapter 5. Direct Thiolysis on Crude Apple Materials for High-Performance Liquid Chromatography Char57
  • Chapter 6. Enzymes Involved in Hydroxycinnamate Metabolism70
  • Chapter 7. Estimation of Procyanidin Chain Length82
  • Section II: Bioavailability95
  • Chapter 8. Determination of Flavonols in Body Fluids97
  • Chapter 9. Determination of Quantity and Quality of Polyphenol Antioxidants in Foods and Beverages103
  • Chapter 10. Preparation and Characterization of Flavonoid Metabolites Present in Biological Samples115
  • Chapter 11. Caffeic Acid as Biomarker of Red Wine Intake122
  • Chapter 12. Measurement of trans-Resveratrol, (+)-Catechin, and Quercetin in Rat and Human Blood and130
  • Chapter 13. Absorption of trans-Resveratrol in Rats145
  • Section III: Antioxidant Action155
  • Chapter 14. Galvinoxyl Method for Standardizing Electron and Proton Donation Activity157
  • Chapter 15. Structure–Activity Relationships Governing Antioxidant Capacities of Plant Polyphenols166
  • Chapter 16. Antioxidant and Prooxidant Abilities of Foods and Beverages181
  • Chapter 17. Metal Chelation of Polyphenols190
  • Chapter 18. Mechanism of Antioxidant Effect of Catechins203
  • Chapter 19. Free Radical Scavenging by Green Tea Polyphenols217
  • Chapter 20. Polyphenol Protection of DNA against Damage232
  • Chapter 21. Markers for Low-Density Lipoprotein Oxidation244
  • Chapter 22. Antioxidant Activity of Hydroxycinnamic Acids on Human Low-Density Lipoprotein Oxidation256
  • Chapter 23. Rapid Screening Method for Relative Antioxidant Activities of Flavonoids and Phenolics266
  • Chapter 24. Nitric Oxide Formation in Macrophages Detected by Spin Trapping with Iron–Dithiocarbam273
  • Chapter 25. Redox Cycles of Caffeic Acid with α-Tocopherol and Ascorbate282
  • Chapter 26. DNA Damage by Nitrite and Peroxynitrite: Protection by Dietary Phenols296
  • Chapter 27. Repair of Oxidized DNA by the Flavonoid Myricetin308
  • Section IV: Biological Activity317
  • Chapter 28. Binding of Flavonoids to Plasma Proteins319
  • Chapter 29. Protein Binding of Procyanidins: Studies Using Polyacrylamide Gel Electrophoresis and Fr333
  • Chapter 30. Characterization of Antioxidant Effect of Procyanidins338
  • Chapter 31. Inhibition of in Vitro Low-Density Lipoprotein Oxidation by Oligomeric Procyanidins Pres350
  • Chapter 32. Biological Actions of Oligomeric Procyanidins: Proliferation of Epithelial Cells and Hai361
  • Chapter 33. Effect of Polyphenolic Flavonoid Compounds on Platelets369
  • Chapter 34. Assessing Bioflavonoids as Regulators of NF-kB Activity and Inflammatory Gene Expression380
  • Chapter 35. Interaction between Cultured Endothelial Cells and Macrophages: In Vitro Model for Study387
  • Chapter 36. Determination of Cholesterol-Lowering Potential of Minor Dietary Components by Measuring398
  • Author Index405
  • Subject Index433
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822361
  • ISBN-13 9780080496870

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.
This volume presents an extensive collection of new methodologies to aid progress in solving unanswered questions concerning the bioavailability and metabolism of flavonoids and polyphenols, their biochemical and molecular biological effects on cell regulation, and their effects on health. Major topics in this volume include sources, characterization, analytical methods, bioavailability, antioxidant action, and biological activity.