Elsevier S & T

G Protein Pathways, Part B: G Proteins and Their Regulators: G Proteins and Their Regulators

Iyengar, Ravi

In stock

No reviews

77.750 KD inc. VAT

License

perpetual

Table of contents

Table of Contentsv
Contributors to Volume 343ix
Prefacexv
Volume in Seriesxix
Section I: G Protein-Coupled Receptors1
A. Theoretical Evaluation of Receptor Function1
Chapter 1. Considerations in the Evaluation of Inverse Agonism and Protean Agonism at GProtein-Coupl3
Chapter 2. Theoretical Implications of Receptor Coupling to Multiple G Proteins Based on Analysis of17
B. Design and Use of Receptor Ligands1
Chapter 3. Use of Retinal Analogues for the Study of Visual Pigment Function29
Chapter 4. Design and Synthesis of Peptide Antagonists and Inverse Agonists for G Protein-Coupled Re49
Chapter 5. Design of Peptide Agonists73
Chapter 6. Design of Nonpeptides from Peptide Ligands for Peptide Receptors91
Chapter 7. Strategies for Mapping the Binding Site of the Serotonin 5-HT2A Receptor123
C. Structural Characterization of Receptor Proteins1
Chapter 8. Use of the Substituted Cysteine Accessibility Method to Study the Structure and Function137
Chapter 9. Mass Spectrometric Analysis of GProtein-Coupled Receptors157
Chapter 10. Probing the Higher Order Sructure of G Protein- Coupled Receptors Using Tethered Cleavag162
Chapter 11. Use of Fluorescence Spectroscopy to Study Conformational Changes in the β2-Adrenoceptor170
Chapter 12. Crystallization of Membrane Proteins in Cubo183
Chapter 13. N-Linked Carbohydrates on G Protein-Coupled Receptors: Mapping Sites of Attachment and D200
Chapter 14. Magic Angle Spinning Nuclear Magnetic Resonance of Isotopically Labeled Rhodopsin212
Chapter 15. Use of Nuclear Magnetic Resonance to Study the Three-Dimensional Structure of Rhodopsin223
D. Design and Use of Engineered Receptor Proteins1
Chapter 16. Tools for Dissecting Signaling Pathways in Vivo: Receptors Activated Solely by Synthetic232
Chapter 17. Analysis of Structure–Function from Expression of G Protein-Coupled Receptor Fragments248
Chapter 18. Construction and Analysis of Function of G Protein-Coupled Receptor–G Protein Fusion P260
Chapter 19. Synthetic Gene Technology: Applications to Ancestral Gene Reconstruction and Structure F274
Chapter 20. Considerations in the Design and Use of Chimeric G Protein-Coupled Receptors295
E. Molecular Modeling Studies of Receptor Structure and Function1
Chapter 21. Strategies for Modeling the Interactions of Transmembrane Helices of G Protein-Coupled R313
Chapter 22. Three-Dimensional Representations of G Protein- Coupled Receptor Structures and Mechanis329
F. Analysis of Receptor Protein Coupling1
Chapter 23. Reconstitution of G Protein-Coupled Receptors with Recombinant GProteinαand βγ Subuni372
Chapter 24. Cell-Free Membrane Desensitization Assay for G Protein-Coupled Receptors394
Chapter 25. Methods to Determine the Constitutive Activity of Histamine H2 Receptors405
Chapter 26. Expression of G Protein-Coupled Receptors and G Proteins in Sf9 Cells: Analysis of Coupl417
Chapter 27. G Protein-Coupled Receptors and Proliferative Signaling430
G. Characterization of Receptor Heterogeneity1
Chapter 28. Genetic Analysis of G Protein-Coupled Receptor Genes448
Chapter 29. IdentiIfication of Adrenergic Receptor Polymorphisms459
Chapter 30. Strategies and Requirements for the Detection of RNA Editing in G Protein Coupled-Recept476
H. The Study of Receptor Trafficking1
Chapter 31. Fluorescence MicroscopyTechniques for the Study of G Protein-Coupled Receptor Traffickin492
Chapter 32. Measurement of Receptor Desensitization and Internalization in Intact Cells506
Chapter 33. Morphological and Biochemical Strategies for Monitoring Trafficking of Epitope-Tagged G530
Section II: Regulators of GPCR Function545
A. G Protein-Coupled Receptor Kinases (GRKs)545
Chapter 34. Characterization of G Protein-Coupled Receptor Kinases547
Chapter 35. Regulation of G Protein-Coupled Receptor Kinase 2559
Chapter 36. Rhodopsin and Its Kinase578
B. Arrestins and Novel Proteins545
Chapter 37. Characterization of Arrestin Expression and Function600
Chapter 38. Identification of Novel G Protein-Coupled Receptor-Interacting Proteins611
Author Index623
Subject Index661

Book details

Vendor Elsevier S & T
SKU 9780121822453
ISBN-13 9780080496924
Author Iyengar, Ravi
Category Medical
Subject Biochemistry

Do you have questions about this book?

Ask an expert!

This volume covers topics such as the structure and identification of functional domains of G proteins, and activation of G proteins by receptors or other regulators. The text takes an integrated approach to studying common experimental questions at many different levels related to G proteins. Methods related to G proteins using molecular modeling, systems biology, protein engineering, protein biochemistry, cell biology, and physiology are all accessible in the same volume.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.

Instant delivery by email
Your access email arrives within minutes of checkout, with a sign-in link for each book — no shipping, no waiting.

Read on any device
Books open in VitalSource Bookshelf on your phone, tablet, or computer, online or offline. Your library is always available at aafaq.vitalsource.com — just log in with the email you used at checkout.

Lost the email?
Resend it to yourself in seconds from My eBook orders, or email cs@aafaqeducation.com and we'll help.