Elsevier S & T

G Proteins

Roche, Patrick C.

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Table of contents

Table of Contentsv
Contributorsix
Prefacexv
Volumes in Seriesxvii
Chapter 1. G Proteins Controlling Differentiation, Growth, and Development: Analysis by Antisense RN1
Chapter 2. Analysis of Adenylylcyclase Subspecies Gene Expression in Brain by in Situ Hybridization19
Chapter 3. Site-Directed Mutagenesis of G Protein a Subunits: Analysis of αz Phosphorylation and Fa34
Chapter 4. G Protein Activity in Solubilized Membrane Preparations50
Chapter 5. Cross-Linking of Synaptoneurosome G Proteins58
Chapter 6. Quantitative Reconstitution and ADP-Ribosylation Assays for G Proteins64
Chapter 7. Functional Assays to Study Coupling of Dopamine D2 Receptors to G Proteins75
Chapter 8. Assays for G Protein βγ Subunit Activity90
Chapter 9. α2–Adrenergic Receptor Coupling to G Proteins101
Chapter 10. Somatostatin Receptor Coupling to G Proteins120
Chapter 11. Galanin Receptor–G Protein Interactions and Stimulation of GTPase Activity133
Chapter 12. Cloning and Site-Directed Mutagenesis Studies of Gonadotropin-Releasing Hormone Receptor143
Chapter 13. Use of the Xenopus laevis Expression System for Structure–Function Analysis of G-Prote170
Chapter 14. Production of G Protein α–Subunit-Specific Monoclonal Antibodies187
Chapter 15. Expression and Purification of G Protein βγ, Subunits Using Baculovirus Expression Sys207
Chapter 16. Analysis of G Protein γ Subunits Using Baculovirus Expression System: Requirement for P227
Chapter 17. Expression, Purification, and Functional Reconstitution of Recombinant Phospholipase C-246
Chapter 18. Measuring Cooperative Binding between Retinal Rod G Protein, Transducin, and Activated R264
Chapter 19. Crosstalk between Tyrosine Kinase and G-Protein-Linked Signal Transduction Systems280
Chapter 20. Subcellular Distribution and Posttranslational Modifications of GTP-Binding Proteins in298
Chapter 21. Epidermal Growth Factor-Mediated Regulation of G Proteins and Adenylylcyclase in Cardiac319
Chapter 22. G Protein Dependence of α1–Adrenergic Receptor Subtype Action in Cardiac Myocytes344
Chapter 23. Gsα Mutations and Pituitary Adenomas362
Chapter 24. Molecular Methods for Analysis of Genetic Polymorphisms: Application to the Molecular Ge379
Index401

Book details

Vendor Elsevier S & T
SKU 9780121852993
ISBN-13 9780080532271
Author Roche, Patrick C.
Category Medical
Subject Biochemistry

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