Elsevier S & T

Guide to Yeast Genetics and Molecular Cell Biology, Part B

Guthrie, Christine

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 350ix
  • Prefacexv
  • Volume in Seriesxvii
  • Section I: Basic Techniques1
  • Chapter 1. Getting Started with Yeast3
  • Chapter 2. How to Set up a Yeast Laboratory42
  • Chapter 3. Constructing Yeast Libraries72
  • Chapter 4. Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glyco87
  • Chapter 5. Genetic Transformation of Saccharomyces cerevisiae Mitochondria97
  • Chapter 6. LacZ Assays in Yeast112
  • Chapter 7. Analysis of Budding Patterns131
  • Chapter 8. Uses and Abuses of HO Endonuclease141
  • Chapter 9. Assays for Gene Silencing in Yeast165
  • Section II: Making Mutants187
  • Chapter 10. Classical Mutagenesis Techniques189
  • Chapter 11. Introduction of Point Mutations into Cloned Genes199
  • Chapter 12. Insertional Mutagenesis: Transposon–Insertion Libraries as Mutagens in Yeast219
  • Chapter 13. Tn7-Mediated Mutagenesis of Saccharomyces cerevisiae Genomic DNA in Vitro230
  • Chapter 14. Vector Systems for Heterologous Expression of Proteins in Saccharomyces cerevisiae248
  • Chapter 15. Cloning-Free Genome Alterations in Saccharomyces cerevisiae Using Adaptamer-Mediated PCR258
  • Chapter 16. kar-Mediated Plasmid Transfer between Yeast Strains: Alternative to Traditional Transfor278
  • Chapter 17. Gene Disruption290
  • Chapter 18. Synthetic Dosage Lethality316
  • Section III: Genomics327
  • Chapter 19. Saccharomyces Genome Database329
  • Chapter 20. Three Yeast Proteome Databases: PombePD, and CalPD (MycoPathPD)347
  • Chapter 21. Database Resources Relevant to Yeast Biology373
  • Chapter 22. Searching Yeast Intron Data at Ares Lab Web Site380
  • Chapter 23. Yeast Genomic Expression Studies Using DNA Microarrays393
  • Chapter 24. Transcriptome Analysis of Saccharomyces cerevisiae Using Serial Analysis of Gene Express414
  • Chapter 25. PCR-Based Engineering of Yeast Genome445
  • Chapter 26. ChIP-chip: A Genomic Approach for Identifying Transcription Factor Binding Sites469
  • Chapter 27. Computational Approaches to Identifying Transcription Factor Binding Sites in Yeast Geno484
  • Section IV: Proteomics497
  • Chapter 28. Array-Based Methods for Identifying Protein Protein and Protein–Nucleic Acid Interacti499
  • Chapter 29. Building Protein–Protein Networks by Two- Hybrid Mating Strategy513
  • Chapter 30. Integrated Version of Reverse Two-Hybrid System for the Postproteomic Era525
  • Chapter 31. Biochemical Genomics Approach to Map Activities to Genes546
  • Chapter 32. Use of Two-Dimensional Gels in Yeast Proteomics559
  • Author Index585
  • Subject Index609
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822538
  • ISBN-13 9780080496986
  • Author Guthrie, Christine
  • Category Science
  • Subject Biochemistry

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This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics.