Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas: Molecular Genetics; Lung and Breast Carcinomas
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Table of contents
- Contentsvii
- Authors and Coauthors of Volume 1xi
- Forewordxv
- Preface to Volume 1xvii
- Preface to Volumes 2 and 3xix
- Contents of Volumes 2 and 3xxi
- Introduction to Volumes 2 and 3xxv
- Prologuexxvii
- Selected Definitionsxxix
- Classification Scheme of Human Cancersxxxiii
- Lung and Breast Carcinomasxxxv
- I. Introduction1
- 1.1 Comparison of Immunohistochemistry, in situ Hybridization, Fluorescence in situ Hybridization, a3
- 1.2 Comparison of Chromogenic in situ Hybridization, Fluorescence in situ Hybridization, and Immunoh13
- 1.3 Target and Signal Amplification to Increase the Sensitivity of in situ Hybridization27
- II. Molecular Pathology43
- 2.1 Polymerase Chain Reaction Technology45
- 2.2 DNA Microarrays Technology49
- 2.3 Tissue Microarrays and Their Modifications in High-throughput Analysis of Clinical Specimens57
- 2.4 Gene Expression Profiling Using Laser Microdissection in Cancer Tissues67
- 2.5 Differential Display of Gene Expression in Human Carcinomas75
- 2.6 Serial Analysis of Gene Expression in Human Diseases85
- III. Lung Carcinoma99
- 3.1 Lung Carcinoma: An Introduction101
- 3.2 Histopathological Classification and Phenotype of Lung Tumors105
- 3.3 Immunohistochemistry and in situ Hybridization of Mucin in Lung Carcinoma115
- 3.4 Immunohistochemical Expression of MDM2 in Lung Carcinoma127
- 3.5 Immunohistochemical Expression of E2F1 and p14ARF in Lung Carcinoma133
- 3.6 Role of Immunohistochemical Expression of Beta-Catenin in Lung Carcinoma141
- 3.7 Immunohistochemistry of Laminin-5 in Lung Carcinoma155
- 3.8 Role of Immunohistochemical Expression of Caveolin-1 in Lung Carcinoma163
- 3.9 Role of Thyroid Transcription Factor-1 in Pulmonary Adenocarcinoma169
- 3.10 Role of Global Methylation of DNA in Lung Carcinoma181
- 3.11 Immunohistochemical and Molecular Pathology of Angiogenesis in Primary Lung Adenocarcinoma189
- 3.12 Immunohistochemistry of Human Leukocyte Antigen Expression in Lung Carcinoma199
- 3.13 Immunohistochemistry and in situ Hybridization of Telomerase in Lung Carcinoma205
- 3.14 Use of Fluorescence in situ Hybridization in Detection of Lung Cancer Cells213
- 3.15 Immunohistochemistry of BCL-2 Gene Expression in Lung Carcinoma223
- IV. Breast Carcinoma231
- 4.1 Breast Carcinoma: An Introduction233
- 4.2 Expression of Vascular Endothelial Growth Factor Receptor-2/Flk-1/KDR in Breast Carcinoma249
- 4.3 HER-2/neu Amplification and Protein Overexpression in Breast Carcinoma: Immunohistochemistry and259
- 4.4 HER-2/neu Amplification Detected by Fluorescence in situ Hybridization in Cytological Samples fr267
- 4.5 Detection of HER-2 Oncogene in Human Breast Carcinoma Using Chromogenic in situ Hybridization279
- 4.6 Immunohistochemical Evaluation of Sentinel Lymph Nodes in Breast Carcinoma289
- 4.7 CD10 Expression in Normal Breast and Breast Cancer Tissues299
- 4.8 Role of Immunohistochemical Expression of AKT Protein in Breast Carcinoma307
- 4.9 Expression of Extracellular Matrix Proteins in Breast Cancer321
- 4.10 Immunohistochemistry of Adhesion Molecule CEACAM1 Expression in Breast Carcinoma337
- 4.11 Role of Cadherins in Breast Cancer343
- 4.12 Immunohistochemical Expression of Erythropoietin and Erythropoietin Receptor in Breast Carcinom351
- 4.13 Loss of BRCA1 Gene Expression in Breast Carcinoma361
- 4.14 Role of Immunohistochemical Defection of BRCA1 in Breast Cancer371
- 4.15 Fluorescence in situ Hybridization of BRCA1 Gene in Breast Carcinoma385
- 4.16 Immunohistochemistry of c- myc Expression in Breast Carcinoma395
- 4.17 Immunohistochemical Localization of Neuropilin-1 in Human Breast Carcinoma: A Possible Molecula409
- 4.18 Role of the Epidermal Growth Factor Receptor in Breast Carcinoma415
- 4.19 Alterations of the Cell Cycle Regulating Proteins in Invasive Breast Cancer: Correlation with P425
- 4.20 Immunohistochemistry of Estrogen Receptor Expression in Breast Carcinoma439
- 4.21 Immunofluorescence and Immunohistochemical Localization of Progesterone Receptors in Breast Car449
- 4.22 Immunohistochemical Expression of Cytosolic Thymidine Kinase in Patients with Breast Carcinoma463
- 4.23 Immunohistochemical Detection of Melanoma Antigen E (MAGE) Expression in Breast Carcinoma471
- 4.24 Role of Immunohistochemical Expression of Receptors in Male Breast Carcinoma477
- 4.25 Detection of Glycoconjugates in Breast Cancer Cell Lines: Confocal Fluorescence487
- 4.26 Role of ETV6-NTRK3 Gene Fusion in Breast Carcinoma493
- 4.27 Role of CA6 Protein Expression in Breast Carcinoma505
- 4.28 Immunohistochemistry of Effusions513
- 4.29 Immunohistochemistry of Needle Cytopunctures of Breast Carcinomas523
- Index533
The various cell types have traditionally been recognized and classified according to their appearance in the light microscope following the process of fixing, processing, sectioning, and staining tissues that is known as histology. Classical histology has been augmented by
immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.
This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.
* The only book available that translates molecular genetics into cancer diagnosis
* Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis
* Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques
* Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis
* Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer
* Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA
* Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References
immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.
This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today, ensuring a wide audience for this book.
* The only book available that translates molecular genetics into cancer diagnosis
* Provides the readers with tools necessary to perform and optimize sensitive, powerful techniques, including immunohistochemistry and fluorescence in situ hybridization, used in tumor diagnosis
* Written by experts in this field, the book provides theoretical considerations as well as practical approaches to carry out effectively these techniques
* Offers suggestions, tips, cautions, and guidelines to avoid artifacts and misdiagnosis
* Introduces new techniques to detect genes and proteins involved in the initiation and progression of cancer
* Covers the latest developments and a wide range of applications to the detection of antigens and single-copy DNA and RNA
* Written in a uniform format, each chapter includes Introduction, Materials required, step-by-step detailed Methods, Results, Discussion, and comprehensive up-to-date References