High Performance Liquid Chromatography & Capillary Electrophoresis: Principles and Practices
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Table of contents
- Contentsvii
- Prefacexiii
- Chapter 1. High-Performance Liquid Chromatography1
- 1.1 Introduction1
- 1.2 Classification of Liquid Chromatographic Methods2
- 1.3 Basic Concepts of HPLC7
- 1.4 Summary of Major Concepts20
- References21
- Chapter 2. Separations in High-Performance Liquid Chromatography24
- 2.1 Introduction24
- 2.2 Normal-Phase Chromatography24
- 2.3 Reversed-Phase Chromatography29
- 2.4 Hydrophobic-Interaction Chromatography38
- 2.5 Ion-Exchange Chromatography38
- 2.6 Size-Exclusion Chromatography46
- 2.7 Affinity Chromatography50
- 2.8 Chiral Separations57
- 2.9 Summary of Major Concepts62
- References67
- Chapter 3. Instrumentation for High-Performance Liquid Chromatography71
- 3.1 Introduction71
- 3.2 Solvent Delivery Systems71
- 3.3 Solvent Degassing78
- 3.4 Isocratic versus Gradient Pumping Systems80
- 3.5 Sample Introduction83
- 3.6 Columns85
- 3.7 Detectors90
- 3.8 Sample Preparation109
- 3.9 Troubleshooting117
- 3.10 Summary of Major Concepts129
- References130
- Chapter 4. Capillary Electrophoresis134
- 4.1 Introduction134
- 4.2 Classification of Electrophoretic Modes134
- 4.3 Basic Concepts of Capillary Electrophoresis136
- 4.4 Summary of Major Concepts150
- References152
- Chapter 5. Separation in Capillary Electrophoresis154
- 5.1 Introduction154
- 5.2 Capillary Zone Electrophoresis155
- 5.3 Micellar Electrokinetic Capillary Chromatography161
- 5.4 Capillary Gel Electrophoresis165
- 5.5 Capillary Electrochromatography170
- 5.6 Chiral Capillary Electrophoresis172
- 5.7 Capillary Isoelectric Focusing174
- 5.8 Capillary Isotachophoresis178
- 5.9 Summary of Major Concepts182
- References183
- Chapter 6. Instrumentation of Capillary Electrophoresis185
- 6.1 Introduction185
- 6.2 High-Voltage Power Supply186
- 6.3 Sample Injection187
- 6.4 Capillaries190
- 6.5 Detection193
- 6.6 Fraction Collection205
- 6.7 Sample Preparation205
- 6.8 Troubleshooting206
- 6.9 Summary of Major Concepts210
- References211
- Chapter 7. Data Manipulation214
- 7.1 Introduction214
- 7.2 Identification of Peaks214
- 7.3 Quantitation222
- 7.4 Summary of Major Concepts239
- References240
- Chapter 8. Miniaturization242
- 8.1 Introduction242
- 8.2 Classification of Columns242
- 8.3 Theoretical and Practical Considerations245
- 8.4 Applications of Miniaturized Liquid Chromatography254
- 8.5 Separations on a Chip259
- 8.6 Summary of Major Concepts270
- References271
- Index275
HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity.
Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes.
Key Features
* Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques
* Applicable to a wide variety of separation problems
* Covers basic concepts governing any separation as well as instrumentation and how to use it
* Helps the user to obtain optimal resolution in minimal time
* Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation
* Includes information on upcoming trends such as miniaturization
* Major concepts in each chapter are organized to allow access to information easily and quickly
* Contains practical bibliography for accessing the literature
Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes.
Key Features
* Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques
* Applicable to a wide variety of separation problems
* Covers basic concepts governing any separation as well as instrumentation and how to use it
* Helps the user to obtain optimal resolution in minimal time
* Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation
* Includes information on upcoming trends such as miniaturization
* Major concepts in each chapter are organized to allow access to information easily and quickly
* Contains practical bibliography for accessing the literature