Human Brain Proteome
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Table of contents
- Title Pageiii
- Copyright Pageiv
- Contentsv
- Contents of Recent Volumes325
- Contributorsix
- SECTION I: HIGH-THROUGHPUT TECHNOLOGIES1
- Chapter 1. Biomarker Discovery Using Molecular Profiling Approaches3
- I. Introduction3
- II. Transcriptomics4
- III. Proteomics Methodologies for Biomarker Discovery7
- IV. Principles of SELDI-TOF-MS12
- V. SELDI-TOF-MS in Clinical Proteomics Research17
- VI. Biomarkers in Specific Diseases18
- VII. Metabolomics25
- VIII. Conclusions26
- References27
- Chapter 2. Proteomic Analysis of Mitochondrial Proteins31
- I. Introduction31
- II. Model Systems32
- III. Technological Approaches33
- IV. Differential Expression of Proteins in Mouse Brain Mitochondria from Cortex and Synaptosomes.38
- V. Conclusions43
- References44
- SECTION II: PROTEOMIC APPLICATIONS49
- Chapter 3. NMDA Receptors, Neural Pathways, and Protein Interaction Databases51
- I. Introduction51
- II. NMDA Receptor52
- III. Molecular Clustering55
- IV. Signal Transduction Pathways61
- VI. Proteomic Databases67
- VII. Future Directions73
- References74
- Chapter 4. Dopamine Transporter Network and Pathways79
- I. Introduction79
- II. Structure and Function of DAT80
- III. Cellular Localization of DAT81
- IV. Regulation of DAT81
- V. Mass Spectrometry-Based Proteomics83
- VI. Isolation and Validation of the DAT Protome84
- VII. Mass Spectrometry85
- VIII. Confirmation of Members of the DAT Proteome85
- IX. In Silico Analysis of DAT Proteome90
- References93
- Chapter 5. Proteomic Approaches in Drug Discovery and Development97
- I. Proteomics in Drug Discovery and Development97
- II. Using Proteomics to Identify Biomarkers of Alzheimer’s Disease: A Case Study104
- III. Future Challenges and Conclusions120
- References121
- SECTION III: INFORMATICS127
- Chapter 6. Proteomic Informatics129
- I. What is Proteomics?129
- II. Proteomic Informatics131
- III. Mass Spectrometry and Shotgun Proteomics131
- IV. Identifying Proteins134
- V. Post-hoc Validation of Protein Identification Program Output139
- VI. Quantification141
- VII. Detection of Protein Isoforms142
- VIII. Systems and Workflow Issues146
- IX. Conclusion150
- X. Appendix: List of Mentioned Algorithms by Topic150
- References154
- SECTION IV: CHANGES IN THE PROTEOME BY DISEASE159
- Chapter 7. Proteomics Analysis in Alzheimer’s Disease: New Insights into Mechanisms of Neurodegene161
- I. Introduction161
- II. Proteomics Tools162
- III. Proteomic Studies in AD170
- IV. Proteomics Analysis of Transgenic Models of AD181
- V. Future of Proteomics in AD182
- References182
- Chapter 8. Proteomics and Alcoholism189
- I. Introduction189
- II. Proteomics of the Hippocampus and Nucleus Accumbens192
- III. Future Directions203
- IV. Conclusion211
- References211
- Chapter 9. Proteomics Studies of Traumatic Brain Injury215
- I. Introduction215
- II. Traumatic Brain Injury216
- III. Proteomics Analysis Overview221
- IV. Protein Separation Methods221
- V. Protein Identification and Quantification Methods225
- VI. TBI Proteomics Bioinformatics232
- VII. Prospective Utilities of TBI Proteomics Data237
- References237
- Chapter 10. Influence of Huntington’s Disease on the Human and Mouse Proteome241
- I. Introduction241
- II. Neurodegenerative Disorders Caused by Elongated Poly-Glutamine Repeats242
- III. Approach for an HD Proteomics Study248
- IV. Materials and Methods248
- V. Proteomics Study of HD249
- VI. Discussion270
- VII. Conclusion277
- References278
- SECTION V: OVERVIEW OF THE NEUROPROTEOME285
- Chapter 11. Proteomics„Application to the Brain287
- I. Introduction287
- II. Potential of Proteomics288
- III. Methods in Proteome Analysis289
- IV. Proteome Analysis in Neurosciences299
- V. Administrative Realization of Neuroproteomics303
- References307
- Index313
Proteomics is a systematic approach for studying the identity and function of all proteins expressed in a cell, tissue or organ. New drug targets for diseases are often identified by comparing the proteome of the disease state to the normal state. As a result, proteomics has become increasingly important in the pharmaceutical and biotechnology industries as well as academics. This book contains five sections encompassing the research aspects of proteomics on the brain including the most recent advances in the technology and informatics. It discusses advances in high-throughput proteomic technologies and their application to studying neurological disorders such as Alzheimer’s disease, alcoholism, trauma/stroke, Huntington's disease, and Parkinson's disease. With numerous illustrations to explain the concepts, it provides a comprehensive review on the topic.
* Describes the latest databases and techniques for analyzing the data generated by proteomics
* Outlines the latest developments in proteomic methods
* Provides numerous color illustrations highlighting the application of proteomics to the identification of novel drug targets and biomarkers
* Describes the latest databases and techniques for analyzing the data generated by proteomics
* Outlines the latest developments in proteomic methods
* Provides numerous color illustrations highlighting the application of proteomics to the identification of novel drug targets and biomarkers