Elsevier S & T

Laser Capture in Microscopy and Microdissection: Methods in Enzymology

Conn, P. Michael

In stock
No reviews
74.000 KD inc. VAT
License
Table of contents
  • Table of Contentsv
  • Contributors to Volume 356ix
  • Prefacexiii
  • Volumes in Seriesxv
  • Section I: Basic Principles1
  • Chapter 1. Comparison of Current Equipment3
  • Chapter 2. Laser Capture Microdissection and Its Applications in Genomics and Proteomics12
  • Chapter 3. Going in Vivo with Laser Microdissection25
  • Chapter 4. Use of Laser Capture Microdissection to Selectively Obtain Distinct Populations of Cells33
  • Chapter 5. Optimized Tissue Processing and Staining for Laser Capture Microdissection and Nucleic Ac49
  • Chapter 6. Fluorescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections63
  • Chapter 7. Immunoblotting of Single Cell Types Isolated from Frozen Sections by Laser Microdissectio70
  • Chapter 8. Noncontact Laser Catapulting: A Basic Procedure for Functional Genomics and Proteomics80
  • Chapter 9. Internal Standards for Laser Microdissection99
  • Chapter 10. Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tiss114
  • Section II: Specialized Uses127
  • Chapter 11. Use of Laser Capture Microdissection for Clonal Analysis129
  • Chapter 12. Laser Capture Microdissection in Carcinoma Analysis136
  • Chapter 13. Laser Capture Microdissection to Assess Development144
  • Chapter 14. Application of Laser Capture Microdissection to Proteomics156
  • Chapter 15. Laser Capture Microdissection of Mouse Intestine: Characterizingm mRNA and Protein Expre166
  • Chapter 16. Laser Capture Microdissection in Pathology195
  • Chapter 17. Use of Laser Capture Microscopy in the Analysis of Mouse Models of Human Diseases206
  • Chapter 18. Use of Laser Microdissection in Complex Tissue215
  • Chapter 19. Assessment of Clonal Relationships in Malignant Lymphomas223
  • Chapter 20. Comparison of Normal and Tumor Cells by Laser Capture Microdissection239
  • Chapter 21. Analysis of Folliculostellate Cells by Laser Capture Microdissection and Reverse Transcr247
  • Section III: Genetic Applications256
  • Chapter 22. Analysis of Gene Expression258
  • Chapter 23. Analysis of Specific Gene Expression270
  • Chapter 24. Gene Discovery with Laser Capture Microscopy281
  • Chapter 25. DNA Fingerprinting from Cells Captured by Laser Microdissection288
  • Chapter 26. Single Cell PCR in Laser Capture Microscopy294
  • Chapter 27. Assessment of Genetic Heterogeneity in Tumors Using Laser Capture Microdissection301
  • Chapter 28. Gene Mutations: Analysis in Proliferative Prostatic Diseases Using Laser Capture Microdi308
  • Chapter 29. Use of Laser Capture Microdissection-Generated Targets for Hybridization of High-Density322
  • Chapter 30. Single Cell Gene Mutation Analysis Using Laser- Assisted Microdissection of Tissue Secti333
  • Chapter 31. Methylation in Gene Promoters: Assessment after Laser Capture Microdissection342
  • Author Index353
  • Subject Index375
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822590
  • ISBN-13 9780080497013
  • Author Conn, P. Michael
  • Category Science
  • Subject Research & Methodology

Do you have questions about this book?

Ask an expert!

Description eBook delivery & access Reviews
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.

Major Sections Include:
* Basic Principles, Specialized Uses, and Genetic Applications
* LCM and its application in genomics and proteomics * Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections
* Noncontact laser catapulting for the functional genomics and proteomics
* Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more.