Elsevier S & T

Lectins: Analytical Technologies: Analytical Technologies

Nilsson, Carol L.

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Table of contents
  • Cover
  • Table of Contentsv
  • Prefacevii
  • List of Contributorsix
  • Chapter 1. Lectins: Analytical Tools from Nature1
  • 1. Introduction1
  • 2. A Short History of Lectins4
  • 3. Lectin Structure and Function6
  • 4. Lectins as Analytical Tools7
  • 5. Lectin Resources8
  • Acknowledgments11
  • References12
  • Chapter 2. Crystallography and Lectin Structure Database15
  • 1. Introduction15
  • 2. X-Ray Crystallography15
  • 3. Lectin Databases28
  • 4. Recent Research Highlights32
  • 5. Hands-on Protocols and Practical Tips37
  • References45
  • Chapter 3. NMR Investigations of Lectin–Carbohydrate Interactions51
  • 1. Introduction51
  • 2. Protein Expression and Purification52
  • 3. Fast or Slow Exchange56
  • 4. How to Track Chemical Shift Changes57
  • 5. How to Obtain 3D Structures of Lectin–Carbohydrate Complexes63
  • 6. Transferred NMR Methods66
  • 7. Emerging NMR Methodologies69
  • 8. Summary70
  • References70
  • Chapter 4. Applications of Isothermal Titration Calorimetry to Lectin–Carbohydrate Interactions75
  • 1. Introduction75
  • 2. Characterization of the Binding Specificity and Binding Sites of Lectins77
  • 3. Binding of Multivalent Carbohydrates to Lectins89
  • Acknowledgments99
  • References99
  • Chapter 5. Bioanalytical Studies Based on Lectin–Carbohydrate Interactions Measured by Ellipsometr103
  • 1. Introduction103
  • 2. Ellipsometry120
  • References127
  • Chapter 6. Use of Natural and Synthetic Oligosaccharide, Neoglycolipid and Glycolipid Libraries in D129
  • 1. Introduction129
  • 2. Analytical Technologies for Analysis of Carbohydrate Pathogen Binding132
  • 3. Carbohydrate Libraries136
  • 4. Libraries in Analysis of Novel H. pylori Binding Specificities143
  • 5. Poly-N-Acetyllactosamine Receptors for Influenza Virus151
  • References162
  • Chapter 7. Carbohydrate Microarrays for Lectin Characterization and Glyco-Epitope Identification167
  • 1. Introduction167
  • 2. The Specificity-Based Lectin Classification168
  • 3. Carbohydrate Microarrays180
  • 4. Exploring Lectin-Binding Profiles Using Carbohydrate Microarrays185
  • References189
  • Chapter 8. Glycoproteomics Based on Lectin Affinity Chromatographic Selection of Glycoforms193
  • 1. Proteomic Strategies193
  • 2. Recognizing Post-Translational Modifications194
  • 3. Lectin Selectors195
  • 4. Serial Lectin Affinity Chromatography197
  • 5. Proteomic Strategies Based on Lectin Selection199
  • 6. The Future210
  • References210
  • Chapter 9. Miniaturized Lectin Affinity Chromatography213
  • 1. Introduction213
  • 2. Principle of Miniaturized LAC214
  • 3. Preparation and Applications of Miniaturized LAC215
  • 4. Perspective236
  • Acknowledgment236
  • References237
  • Chapter 10. Frontal Affinity Chromatography: Systematization for Quantitative Interaction Analysis B239
  • 1. Introduction239
  • 2. General Features and Principle240
  • 3. Operation247
  • 4.Applications257
  • 5. Concluding Remarks265
  • References265
  • Chapter 11. Technical Aspects of Glycoprotein Enrichment267
  • 1. Introduction267
  • 2. Practical Aspects of Lectin Affinity Chromatography269
  • 3. Lectins Immobilized to Agarose Gels273
  • 4. Lectins Immobilized to Porous Silica278
  • 5. Lectins Immobilized to Monoliths286
  • 6. Lectins Immobilized to Other Materials290
  • 7. Enrichment with Hydrazide-Activated Particles293
  • 8. Conclusions296
  • References297
  • Chapter 12. Proteomic Techniques for Functional Identification of Bacterial Adhesins299
  • 1. Introduction299
  • 2. Protein Separation Techniques300
  • 3. Biological Mass Spectrometry303
  • 4. Bacterial Adhesins and Virulence311
  • 5. Proteomic Analysis of H. pylori314
  • 6. Functional Identification a Lactoferrin-Binding Protein of H. pylori315
  • Acknowledgments321
  • References321
  • Chapter 13. The Use of Lectins in Bioaffinity MALDI Probes327
  • 1. Introduction and Background327
  • 2. A General Method for Producing Bioaffinity MALDI Probes327
  • 3. Identification and Structure Elucidation of Lectin-Binding Oligosaccharides by Bioaffinity MALDI332
  • References342
  • Chapter 14. Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for343
  • 1. Introduction343
  • 2. ESI FT-ICR MS Instrumentation344
  • 3. FT-ICR MS Operating Principles347
  • 4. Performance of FT-ICR MS350
  • 5. Top-Down and Bottom-Up Proteomics352
  • 6. Tandem Mass Spectrometry in ESI FT-ICR MS353
  • 7. Negative Ion Mode Tandem Mass Spectrometry362
  • 8. Metal-Assisted Tandem Mass Spectrometry364
  • 9. Summary365
  • Acknowledgments365
  • References366
  • Chapter 15. Catalytically Inactive Endoglycosidases as Microbial Diagnostic Reagents: Chitinases and373
  • 1. Introduction373
  • 2. Use of E35Q HEWL for Bacterial Diagnostics380
  • References383
  • Chapter 16. Generation of Lectins from Enzymes: Use of Inactive Endosialidase for Polysialic Acid De385
  • 1. Polysialic Acid385
  • 2. Endosialidase-Green Fluorescent Protein Fusion Construct386
  • 3.Applications of the Fusion Protein388
  • 4. Comments391
  • 5. Experimental Considerations392
  • References393
  • Chapter 17. Probing Cell Surface Lectins with Neoglycoconjugates397
  • 1. Introduction397
  • 2. Design of Neoglycoconjugates as Probes398
  • 3. Experimental Approaches398
  • 4. Examples of Application402
  • 5. Discussion408
  • Acknowledgments413
  • References413
  • Appendix 1. Table of Lectin Affinities417
  • References423
  • Appendix 2. List of Abbreviations427
  • Subject Index435
Book details
  • Vendor Elsevier S & T
  • SKU 9780444530776
  • ISBN-13 9780080548661
  • Author Nilsson, Carol L.
  • Category Science
  • Subject Analytic

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Description eBook delivery & access Reviews
Lectins: Analytical Technologies covers both analytical and biological aspects of lectins (functional carbohydrate (complex sugar) recognition proteins) and provides researchers in the field with a resource containing background information and 'look-up' tables detailing lectin specificity and structures. Also included are methods and practical tips for designing new lectins from existing non-lectin proteins, automated approaches to lectin proteomics and high resolution mass spectrometry techniques.



This book will be of interest to both novice and advanced researchers in biomedical, analytical and pharmaceutical fields who are involved in the study of lectin structures or who utilize lectins as analytical tools. The study of lectins and their employment in analytical settings spans a range of fields including:
* Crystallography and lectin structure databases
* Carbohydrate microarrays for lectin characterization and glycotope identification
* Proteomic approaches to the functional identification of bacterial adhesins
* Generation of lectins from enzymes
* Probing cell-surface lectins with neoglycoconjugates

* Reviews up-to-date techniques, including practical hints for laboratory work
* Provides overview of lectin e-resources and several color illustrations
* Includes a 'look-up' table detailing lectin specificity