Elsevier S & T

Measurement and Manipulation of Intracellular Ions

Conn, P. Michael

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Table of contents
  • Table of Contentsvii
  • Contributors to Volume 27ix
  • Prefacexiii
  • Volumes in Seriesxv
  • Chapter 1. Light-Optical-Based Reagents for the Measurement and Manipulation of Ions, Metabolites, a1
  • Chapter 2. Use of Ion-Selective Microelectrodes for Recording Intracellular Ion Levels17
  • Chapter 3. Use of NMR Spectroscopy to Measure Intracellular Ion Concentrations39
  • Chapter 4. Use of Ionophores for Manipulating Intracellular Ion Concentrations52
  • Chapter 5. Fluorescence Imaging of Cytosolic Calcium: An Introduction to Basic Experimental Principl69
  • Chapter 6. Fluorescence Measurements of Cytosolic Calcium: Combined Photometry with Electrophysiolog81
  • Chapter 7. Use of Chelators and Photoactivatable Caged Compounds to Manipulate Cytosolic Calcium123
  • Chapter 8. Measurement of Cytosolic Calcium by 19F NMR153
  • Chapter 9. Fluorescence Measurement of Cytosolic pH in Cultured Rodent Astrocytes196
  • Chapter 10. Brain Cell Acid–Base Changes Measured with Ion-Selective Microelectrodes214
  • Chapter 11. Measurement of Cytosolic pH by Nuclear Magnetic Resonance Spectroscopy233
  • Chapter 12. Manipulation and Regulation of Cytosolic pH252
  • Chapter 13. Fluorescence Measurements of Cytosolic Sodium Concentration274
  • Chapter 14. Measurement of Cytosolic Sodium Using Ion-Selective Microelectrodes289
  • Chapter 15. A Practical Guide to the Use of Fluorescent Indicators for the Measurement of Cytosolic304
  • Chapter 16. Measurement of Cytosolic Magnesium by 31P NMR Spectroscopy319
  • Chapter 17. Chloride-Sensitive Fluorescent Indicators328
  • Chapter 18. Measurement of Cytosolic Chloride Activity by Ion-Selective Microelectrodes340
  • Chapter 19. Use of Ion-Selective Microelectrodes and Fluorescent Probes to Measure Cell Volume361
  • Index393
Book details
  • Vendor Elsevier S & T
  • SKU 9780121852979
  • ISBN-13 9780080536460
  • Author Conn, P. Michael
  • Category Psychology
  • Subject Neuropsychology

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Description eBook delivery & access Reviews
This practical book provides the detailed methodology and expert guidance required for measuring and manipulating cytosolic ion concentrations. In addition, the strengths, weaknesses, and pitfalls of various techniques are presented. It is an invaluable source for those needing an objective evaluation of current methodologies and for those contemplating setting up such procedures.

Key Features
* A one-source reference for measuring and manipulating intracellular ions and for comparing and evaluating current methodologies
* Includes overviews of
* Optical probes and reagents
* Fabrication and use of ion-selective microelectrodes
* Use of NMR spectroscopy
* Ionophores