Measuring Immunity: Basic Science and Clinical Practice
Regular price
92.500 KD
inc. VAT
Couldn't load pickup availability
Table of contents
- Coverii
- Contentsv
- Forewordix
- Prefacexiii
- Contributorsxv
- Section I Fundamentals of the Immune Response1
- 1 MHC Class I3
- 2 MHC Class II12
- 3 Cytokine receptor heterogeneity23
- 4 Genetic diversity at human cytokine loci in health and disease35
- 5 Signaling molecules affecting immune response62
- 6 Toll-like receptors in innate immunity80
- 7 DNA sequence-specific transcription factors91
- 8 Genetic diversity in NK and NKT cells110
- Section II Serologic Assays119
- 9 Handling sera and obtaining fluid from different compartments121
- 10 Acute-phase proteins and inflammation131
- 11 Complement in health and disease144
- 12 Immunoglobulin titers and immunoglobulin subtypes158
- 13 Human antiglobulin responses172
- 14 Rheumatoid factors187
- 15 Autoantibodies193
- 16 Antibody affinity using fluorescence201
- 17 SLE-associated tests210
- 18 Multiplexed serum assays221
- Section III Cellular Enumeration and Phenotyping231
- 19 Handling and storage of cells and sera: practical considerations233
- 20 Phenotypic and functional measurements on circulating immune cells and their subsets237
- 21 Natural killer cells257
- 22 Tetramer analysis268
- 23 Peripheral blood naive and memory B cells277
- 24 Dendritic cells290
- 25 Monocytes and macrophages299
- 26 Tumor cells312
- 27 Regulatory T. cells322
- 28 Intracellular cytokine assays336
- Section IV Cellular Function and Physiology341
- 29 Cytolytic assays343
- 30 Mixed leukocyte reactions350
- 31 Antigen/mitogen-stimulated lymphocyte proliferation361
- 32 Monitoring cell death369
- 33 Cytokine enzyme linked immunosorbent spot (ELISPOT) assay380
- 34 Testing natural killer cells396
- Section V Provocative Assays in vivo405
- 35 Delayed type hypersensitivity responses407
- 36 Rebuck windows: granulocyte function419
- 37 The vascular and coagulation systems428
- 38 Sentinel node assays434
- 39 Imaging inflammation445
- Section VI Assays in Acute and Chronic Diseases463
- 40 Cancer – solid tumors465
- 41 Cancer – hematologic disorders473
- 42 Autoimmunity – rheumatoid arthritis481
- 43 Autoimmunity – type 1 diabetes494
- 44 Autoimmunity – systemic lupus erythematosus505
- 45 Autoimmunity – multiple sclerosis515
- 46 Autoimmunity – inflammatory bowel disease525
- 47 Autoimmunity – endocrine543
- 48 Autoimmunity – vasculitis560
- 49 Transplantation569
- 50 Viral responses – HIV-1578
- 51 Viral responses – epstein-barr virus587
- 52 Viral responses – hepatitis598
- 53 Dermatology610
- 54 Arteriosclerosis620
- 55 Primary immunodeficiencies630
- 56 Asthma and allergy639
- Section VII New Technologies647
- 57 Serum proteomic profiling and analysis649
- 58 Imaging cytometry660
- 59 Cancer biometrics666
- 60 Genomics and microarrays697
- 61 Image informatics707
- Index713
Most of the diseases of modern mankind involve either acute or chronic inflammation. Measuring Immunity integrates the current information available on biomarkers and surrogate assays into a single handbook. It highlights the principles behind various applications, gives a brief summary on how they are conducted and provides detailed and critical analyses of murine models of immunity, clinical trials, and tests to predict utility and benefit. Measuring Immunity is indispensable for scientists and clinicians interested in the clinical applications of modern immunobiology.
* Defines which assays of immune function are helpful in the assessment of clinical disorders involving inflammation and immunity
* Assesses the dynamics of cellular and soluble factors in the peripheral blood using modern techniques
* Includes basic science foundations as well as the approaches currently applied
* Defines which assays of immune function are helpful in the assessment of clinical disorders involving inflammation and immunity
* Assesses the dynamics of cellular and soluble factors in the peripheral blood using modern techniques
* Includes basic science foundations as well as the approaches currently applied