Medical Applications of Mass Spectrometry
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Table of contents
- Cover
- Contentsv
- List of Contributorsxvii
- Prefacexxi
- Part I: Motivation and Essentials1
- Chapter 1. Introduction3
- Chapter 2. Basics of analytical chemistry and mass spectrometry for medical professionals7
- 1. Introduction7
- 2. Terms and definitions9
- 3. The analytical procedure11
- 4. A case study: analysis of plasma sterol profile14
- 5. Mass spectrometry16
- Reference18
- Chapter 3. Ethical, legal, safety, and scientific aspects of medical research19
- 1. Ethical aspects20
- 2. Legal aspects22
- 3. Safety aspects22
- 4. Handling biological materials25
- 5. Clinical trials and protocols28
- 6. Administrative procedures33
- References34
- Part II: Tools of the Trade35
- Chapter 4. Biomedical sampling37
- 1. Sampling37
- 2. Sample preparation40
- 3. Extraction techniques46
- 4. Automation and high throughput57
- 5. Outlook58
- References59
- Chapter 5. Separation methods61
- 1. Introduction61
- 2. Gas chromatography72
- 3. High-performance liquid chromatography77
- 4. Electrophoretic techniques85
- 5. Future trends88
- References89
- Chapter 6. Mass spectrometry instrumentation and techniques93
- 1. Introduction94
- 2. General questions about mass measurement and mass spectrometry96
- 3. Separation techniques: gas chromatography (GC), and high-performance liquid chromatography (HPLC)100
- 4. Ionization methods102
- 5. Mass analyzers119
- 6. Tandem mass spectrometry (MS/MS)130
- 7. Selected terms for clarification135
- Acknowledgment137
- References137
- Chapter 7. Chemoinformatics„multivariate mathematical statistical methods for data evaluation141
- 1. Introduction141
- 2. Data types and data pretreatment143
- 3. Multivariate methods146
- 4. Selected applications of chemometrics162
- Terms and terminology164
- References166
- Part III: Biomolecules171
- Chapter 8. Mass spectrometry in proteomics173
- 1. Introduction173
- 2. Methods in proteomics176
- 3. Outlook190
- References191
- Chapter 9. De novo sequencing of peptides195
- Chapter 10. Protein bioinformatics203
- 1. Highlights for medical professionals203
- 2. Introduction205
- 3. Methodology206
- 4. Discussion214
- 5. Future trends215
- 6. Conclusions219
- Acknowledgments220
- References220
- Chapter 11. Analysis of complex lipidomes223
- 1. Introduction224
- 2. Methodology226
- 3. Phospholipids230
- 4. Acylglycerols234
- 5. Sphingolipids235
- 6. Sterols237
- 7. Medical applications of MS-lipidomics238
- 8. Future trends240
- 9. Conclusions240
- References240
- Part IV: Selected Medical Applications251
- Chapter 12. Medical laboratory practice„possibilities for mass spectrometry253
- 1. Introduction253
- 2. Quality management254
- 3. Inborn errors of metabolism254
- 4. Assessment of in vivo metabolism using stable isotope techniques258
- 5. Conclusions259
- References259
- Chapter 13. Therapeutic drug monitoring and measurement of drug concentrations using mass spectromet263
- 1. Introduction263
- 2. Antiinfection drugs267
- 3. Drugs acting on the central nervous system (CNS)271
- 4. Cardiovascular drugs273
- 5. Anticancer agents276
- 6. Analgesics280
- 7. Miscellaneous drug classes283
- References285
- Chapter 14. Mass spectrometry of infectious pathogens291
- 1. Introduction291
- 2. Methodology295
- 3. Future trends and prospects305
- References305
- Chapter 15. Proteomics of viruses309
- 1. Introduction310
- 2. Virus–host interactions317
- 3. Diagnostics325
- 4. Discussion331
- 5. Future trends334
- 6. Conclusions335
- Acknowledgments336
- References336
- Chapter 16. Neonatal research345
- 1. Introduction346
- 2. Principle of the methodology348
- 3. Quick reference for practical implementation of the methodology349
- 4. Key points of the methodology356
- 5. Ongoing extensions of NBS366
- 6. Conclusions374
- Acknowledgments376
- References376
- Chapter 17. Applications of mass spectrometry in oncology379
- 1. Introduction380
- 2. New methodology„SELDI TOF MS381
- 3. Other relevant methodological challenges384
- 4. Diagnostic oncoproteomics based on SELDI–TOF–MS386
- 5. Representative other applications393
- References398
- Chapter 18. Application of mass spectrometry in brain research: neurotransmitters, neuropeptides, an407
- 1. Introduction407
- 2. Methodology408
- 3. Discussion417
- 4. Future trends420
- 5. Conclusions421
- Acknowledgments421
- References421
- Chapter 19. The human pituitary proteome: clinical applications425
- 1. Introduction426
- 2. The pituitary gland and mass spectrometry: an endocrinologist’s perspective430
- 3. Methodology432
- 4. Discussion446
- 5. Future trends452
- 6. Conclusions454
- Acknowledgments455
- References455
- Chapter 20. Mass spectrometry of proteinous allergens inducing human diseases459
- 1. Introduction459
- 2. Methodology465
- 3. Mass spectrometric identification of proteinous allergens469
- 4. Future trends477
- 5. Conclusions479
- References480
- Chapter 21. Mass spectrometry in clinical treatment487
- 1. Introduction487
- 2. Pediatrics490
- 3. Oncology493
- References499
- Part V: Emerging Areas503
- Chapter 22. Biomarker discovery505
- 1. Introduction506
- 2. Biomarkers in medicine508
- 3. Important definitions509
- 4. Biomarkers discovery and complexity of biological systems510
- 5. Biomarker discovery and omicsŽ512
- 6. The biomarker discovery project512
- 7. Challenges in the biomarker discovery pathway516
- 8. Technical issues in experimental design522
- 9. The discovery process overview526
- 10. Conclusions530
- References531
- Chapter 23. Molecular imaging by mass spectrometry533
- 1. Introduction533
- 2. Methods535
- 3. Discussion547
- 4. Future trends549
- 5. Conclusion550
- Acknowledgments551
- References551
- Chapter 24. Brief outlook555
- 1. Trends in instrumentation556
- 2. Emerging systems approach558
- 3. Mass spectrometry in translational medicine559
- Index561
Mass spectrometry is fast becoming an indispensable field for medical professionals. The mass spectrometric analysis of metabolites and proteins promises to revolutionize medical research and clinical diagnostics. As this technology rapidly enters the medical field, practicing professionals and students need to prepare to take full advantage of its capabilities.
Medical Applications of Mass Spectrometry addresses the key issues in the medical applications of mass spectrometry at the level appropriate for the intended readership. It will go a long way to help the utilization of mass spectrometry in medicine.
The book comprises five parts. A general overview is followed by a description of the basic sampling and separation methods in analytical chemistry. In the second part a solid foundation in mass spectrometry and modern techniques of data analysis is presented. The third part explains how mass spectrometry is used in exploring various classes of biomolecules, including proteins and lipids. In the fourth section mass spectrometry is introduced as a diagnostic tool in clinical treatment, infectious pathogen research, neonatal diagnostics, cancer, brain and allergy research, as well as in various fields of medicine: cardiology, pulmonology, neurology, psychiatric diseases, hemato-oncology, urologic diseases, gastrointestinal diseases, gynecology and pediatrics. The fifth part covers emerging applications in biomarker discovery and in mass spectrometric imaging.
* Provides a broad look at how the medical field is benefiting from advances in mass spectrometry.
* Guides the reader from basic principles and methods to cutting edge applications.
* There is NO comparable book on the market to fill this fast growing field.
Medical Applications of Mass Spectrometry addresses the key issues in the medical applications of mass spectrometry at the level appropriate for the intended readership. It will go a long way to help the utilization of mass spectrometry in medicine.
The book comprises five parts. A general overview is followed by a description of the basic sampling and separation methods in analytical chemistry. In the second part a solid foundation in mass spectrometry and modern techniques of data analysis is presented. The third part explains how mass spectrometry is used in exploring various classes of biomolecules, including proteins and lipids. In the fourth section mass spectrometry is introduced as a diagnostic tool in clinical treatment, infectious pathogen research, neonatal diagnostics, cancer, brain and allergy research, as well as in various fields of medicine: cardiology, pulmonology, neurology, psychiatric diseases, hemato-oncology, urologic diseases, gastrointestinal diseases, gynecology and pediatrics. The fifth part covers emerging applications in biomarker discovery and in mass spectrometric imaging.
* Provides a broad look at how the medical field is benefiting from advances in mass spectrometry.
* Guides the reader from basic principles and methods to cutting edge applications.
* There is NO comparable book on the market to fill this fast growing field.