Microbial Gene Techniques: Molecular Microbiology Techniques Part B
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Table of contents
- Table of Contentsv
- Contributors to Volume 6ix
- Prefacexv
- Volumes in Seriesxvii
- Section I: Molecular Genetics of Eukaryotic Microbes1
- Chapter 1. Forward and Reverse Genetics in the Study of the Obligate, Intracellular Parasite Toxopla3
- Chapter 2. Techniques Associated with Protozoan Parasite Genome Analysis30
- Chapter 3. Aspergillus nidulans as a Model System for Cell and Molecular Biology Studies48
- Chapter 4. Special Molecular Techniques for Study of Filamentous Fungi66
- Section II: Yeast Chromosomes: Transcription, Recombination, Replication79
- Chapter 5. In Vitro Fragmentation of Yeast Chromosomes and Yeast Artificial Chromosomes at Artificia81
- Chapter 6. Basic Analysis of Transcription Factor Binding to Nucleosomes108
- Chapter 7. Experimental Analysis of Transcription Factor–Nucleosome Interactions129
- Chapter 8. In Vivo Analysis of Nucleosome Structure and Transcription Factor Binding in Saccharomyce153
- Chapter 9. Mapping of Yeast Nucleosomes in Vivo168
- Chapter 10. Novel Assays for Ligation and Cleavage Activities of Site-Specific Recombinases186
- Chapter 11. Physical Monitoring of Mitotic and Meiotic Recombination in Saccharomyces cerevisiae204
- Chapter 12. Genetic Dissection of Complex Heteromultimeric Enzymes: The Case of Yeast DNA-Dependent227
- Chapter 13. Pheromone Procedures in Fission Yeast247
- Section III: Bacterial Gene Structure and Regulation265
- Chapter 14. Use of DNA Methylation Deficient Strains in Molecular Genetics267
- Chapter 15. Identifying the Ends of Infrequent RNA Molecules in Bacteria280
- Chapter 16. Identification of rcs Genes in Escherichia coli 09:K30:H12 and Involvement in Regulation301
- Chapter 17. Genetic Mapping in Bacillus subtilis323
- Chapter 18. Molecular and Biochemical Methods for Studying Chemotaxis in Bacillus subtilis339
- Chapter 19. Cloning of Thermomonospora fusca Cellulase Genes in Escherichia coli and Streptomyces li367
- Chapter 20. Computational Analyses Aiding Identification and Characterization of Proteins, Genes, an375
- Section IV: Plasmids and Phages: Replication,Transcription, Assembly387
- Chapter 21. Techniques to Isolate Specialized Templates for in Vitro Transcription Assays389
- Chapter 22. Protein–Protein Interactions of DNA-Binding Proteins: Studies on the Replication Initi400
- Chapter 23. Transcriptional Regulators: Protein–DNA Complexes and Regulatory Mechanisms421
- Chapter 24. Use of Coliphage a and Other Bacteriophages for Molecular Genetic Analysis of Erwinia an439
- Chapter 25. Isolation, Purification, and Function of Assembly Intermediates and Subviral Particles o455
- Index475
Microbial Gene Techniques is a practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on microbial cells, particularly eukaryotic microbes and bacteria, as well as plasmids and bacteriophages.
*
* Methods presented for ease of use and ready adaptation to new systems.
* Detailed protocols included for:
* Eukaryotic microbes - protozoan parasites (forward and reverse genetics, genome analysis), filamentous fungi (chromosome and gene analysis)
* Yeast chromosomes - YACs, genome mapping, transcription factors, nucleosomes, recombination, RNA polymerase, pheromones.
* Bacterial gene structure and regulation - E. coli (DNA methylation, mRNA characterization, gene regulation), B Subtilis (genetic mapping, chemotaxis), computer identification of genes.
* Plasmids and bacteriophages - plasmid templates for transcription assays, plasmid replication: bacteriophage transcription, molecular genetic analysis using phages, phage assembly.
*
* Methods presented for ease of use and ready adaptation to new systems.
* Detailed protocols included for:
* Eukaryotic microbes - protozoan parasites (forward and reverse genetics, genome analysis), filamentous fungi (chromosome and gene analysis)
* Yeast chromosomes - YACs, genome mapping, transcription factors, nucleosomes, recombination, RNA polymerase, pheromones.
* Bacterial gene structure and regulation - E. coli (DNA methylation, mRNA characterization, gene regulation), B Subtilis (genetic mapping, chemotaxis), computer identification of genes.
* Plasmids and bacteriophages - plasmid templates for transcription assays, plasmid replication: bacteriophage transcription, molecular genetic analysis using phages, phage assembly.