Elsevier S & T

Molecular and Diagnostic Procedures in Mycoplasmology: Diagnostic Procedures

Tully, Joseph G.

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Table of contents

Cover
Contentsv
Contributorsxi
Prefacexv
Contents of Volume Ixvii
Mollicute–Host Interrelationships: Current Concepts and Diagnostic Implications1
Section A: Diagnostic Genetic Probes23
Chapter A1. Introductory Remarks25
Chapter A2. Oligonucleotide Probes Complementary to 16S rRNA29
Chapter A3. Cloned Genomic DNA Fragments as Probes47
Chapter A4. PCR: Selection of Target Sequences53
Chapter A5. PCR: Preparation of DNA from Clinical Specimens61
Chapter A6. PCR: Amplification and Identification of Products65
Chapter A7. PCR: Application of Nested PCR to Detection of Mycoplasmas75
Chapter A8. PCR: Random Amplified Polymorphic DNA Fingerprinting81
Section B: Immunological Tools87
Chapter B1. Introductory Remarks89
Chapter B2. ELISA in Small Animal Hosts, Rodents, and Birds93
Chapter B3. ELISA in Large Animals105
Chapter B4. ELISA in Human Urogenital Infections and AIDS115
Chapter B5. ELISA in Respiratory Infections of Humans123
Chapter B6. Monoclonal Antibodies as Diagnostic Tools137
Chapter B7. Microimmunofluorescence147
Chapter B8. Immunoblots and Immunobinding151
Chapter B9. Differentiation of Mycoplasma genitalium from Mycoplasma pneumoniae by Immunofluorescenc169
Section C: Antibiotic Sensitivity Testing179
Chapter C1. Introductory Remarks181
Chapter C2. Problems and Opportunities in Susceptibility Testing of Mollicutes185
Chapter C3. Determination of Minimal Inhibitory Concentration189
Chapter C4. Cidal Activity Testing199
Section D: Diagnosis of Specific Diseases205
Chapter D1. Introductory Remarks207
Chapter D2. Laboratory Diagnosis of Mycoplasma pneumoniae Infection211
Chapter D3. Diagnosis of Sexually Transmitted Diseases225
Chapter D4. Diagnosis of Neonatal Infections237
Chapter D5. Mycoplasmas in AIDS Patients247
Chapter D6. Mycoplasma Infections of Cattle255
Chapter D7. Mycoplasma Infections of Goats and Sheep265
Chapter D8. Mycoplasma Infections of Swine275
Chapter D9. Mycoplasma Infections of Poultry283
Chapter D10. Diagnosis of Spiroplasma Infections in Plants and Insects293
Chapter D11. Detection of Phytoplasma Infections in Plants299
Chapter D12. Identification of Mollicutes from Insects313
Section E: Experimental Infections323
Chapter E1. Introductory Remarks325
Chapter E2. Experimental Mycoplasmal Respiratory Infections in Rodents327
Chapter E3. Urogenital Infections in Rodents337
Chapter E4. Experimental Models of Arthritis349
Chapter E5. Experimental Infections in Poultry361
Chapter E6. Experimental Infections of Swine371
Chapter E7. Experimental Infections in Cattle377
Chapter E8. Experimental Infections of Plants by Spiroplasmas385
Chapter E9. Experimental Phytoplasma Infections in Plants and Insects391
Chapter E10. Mycoplasmas and in Vitro Infections of Cell Cultures with HIV399
Section F: Diagnosis of Mycoplasma Infections of Cell Cultures405
Chapter F1. Introductory Remarks407
Chapter F2. Isolation of Mycoplasmas from Cell Cultures by Axenic Cultivation Techniques411
Chapter F3. Detection of Mycoplasmas by DNA Staining and Fluorescent Antibody Methodology419
Chapter F4. Detection of Mycoplasma Infection by PCR431
Chapter F5. Antibiotic Treatment of Mycoplasma-lnfected Cell Cultures439
Chapter F6. Prevention and Control of Mycoplasma Infection of Cell Cultures445
Appendix453
Table I. Genus Mycoplasma and Major Characteristics455
Table II. Genera Ureaplasma and Acholeplasma458
Table III. Genera Anaeroplasma and Asteroleplasma458
Table IV. Genera Entomoplasma and Mesoplasma459
Table V. Group Classification of Genus Spiroplasma460
Index463
Color Plate Section467

Book details

Vendor Elsevier S & T
SKU 9780125838061
ISBN-13 9780080536798
Author Tully, Joseph G.
Edition 2nd
Category Medical
Subject Microbiology

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This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas.

Key Features
* Diagnostic genetic probes
* Immunological tools
* Antibiotic sensitivity testing
* Diagnosis of specific diseases
* Experimental infections
* Diagnosis of mycoplasma infections of cell cultures

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