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Table of contents
- Table of Contentsv
- Contributors to Volume 359ix
- Prefacexv
- Volume in Seriesxvii
- Section I: Detection of Nitric Oxide1
- Chapter 1. Electron Paramagnetic Resonance for Quantitation of Nitric Oxide in Aqueous Solutions3
- Chapter 2. In Vitro Detection of Nitric Oxide and Nitroxyl by Electron Paramagnetic Resonance18
- Chapter 3. Iron Dithiocarbamate as Spin Trap for Nitric Oxide Detection: Pitfalls and Successes27
- Chapter 4. Advanced Spin Trapping of Vascular Nitric Oxide Using Colloid Iron Diethyldithiocarbamate42
- Chapter 5. Simultaneous Detection of pO2 and NO by Electron Paramagnetic Resonance52
- Chapter 6. Electron Paramagnetic Resonance Studies Nitric Oxide in Living Mice66
- Chapter 7. Measuring End Products of Nitric Oxide in Vivo75
- Chapter 8. Guide for the Use of Nitric Oxide (NO) Donors as Probes of the Chemistry of NO and Relate84
- Chapter 9. Amperometric Measurement of Nitric Oxide Using a Polydemethylsiloxane-Coated Electrode105
- Chapter 10. Nitric Oxide Monitoring in Hippocampal Brain Slices Using Electrochemical Methods111
- Chapter 11. Electrochemical Detection of Physiological Nitric Oxide: Materials and Methods125
- Chapter 12. Detection of Nitric Oxide Production by Fluorescent Indicators134
- Chapter 13. Specific Analysis of Nitrate and Nitrite by Gas Chromatography/Mass Spectrometry148
- Chapter 14. Griess Method for Nitrite Measurement of Aqueous and Protein-Containing Samples158
- Chapter 15. Gas-Phase Oxidation and Disproportionation of Nitric Oxide168
- Chapter 16. Detection and Quantification of Nitric Oxide (NO) Synthase-Independent Generation of NO180
- Chapter 17. Nitric Oxide-Dependent Vasodilation in Human Subjects186
- Chapter 18. Antioxidant and Diffusion Properties of Nitric Oxide in Low-Density Lipoprotein200
- Chapter 19. Measurements of Redox Control of Nitric Oxide Bioavailability209
- Section II: Nitrosothiols and Nitric Oxide in Cell Signaling217
- Chapter 20. Reactions of S-Nitrosothiols with L-Ascorbic Acid in Aqueous Solution219
- Chapter 21. Nitrosothiols and S-Nitrosohemoglobin229
- Chapter 22. Nitrosothiol Processing by Platelets238
- Chapter 23. Nitric Oxide-Activated Glutathione Sepharose245
- Chapter 24. Measurement of Protein Nitration and S-Nitrosothiol Formation in Biology and Medicine256
- Chapter 25. S-Glutathionylation of NF-κB Subunit p50268
- Chapter 26. Intercellular Signaling Mediated by Nitric Oxide in Human Glioblastoma Cells280
- Chapter 27. L-Arginine Metabolism in Trypanosoma cruzi in the Regulation of Programmed Cell Death286
- Section III: Nitric Oxide and Mitochondrial Functions303
- Chapter 28. Measurement of Mitochondrial Respiratory Thresholds and the Control of Respiration by Ni305
- Chapter 29. Determination of Nitric Oxide-Induced Effects on Tissue Levels of Glutathione and Mitoch319
- Chapter 30. Pharmacological Regulation of Mitochondrial Nitric Oxide Synthase328
- Chapter 31. Characterization of Mitochondrial Nitric Oxide Synthase339
- Section IV: Peroxynitrite351
- Chapter 32. Peroxynitrite Formation from Biochemical and Cellular Fluxes of Nitric Oxide and Superox353
- Chapter 33. Evaluation of Antioxidant Activity Using Peroxynitrite as a Source of Radicals366
- Chapter 34. Peroxynitrite Reactions with Heme and Heme- Thiolate (P450) Proteins379
- Chapter 35. Gas Chromatography/Mass Spectrometry Assay for 3-Nitrotyrosine390
- Chapter 36. Quantitation and Localization of Tyrosine Nitration in Proteins399
- Section V: Nitric Oxide Synthases411
- Chapter 37. Neuronal Nitric Oxide Synthases in Brain and Extraneural Tissues413
- Chapter 38. Visualization and Distribution of Neuronal Nitric Oxide Synthase-Containing Neurons424
- Chapter 39. Quantitative Measurement of Endothelial Constitutive Nitric Oxide Synthase433
- Chapter 40. Quantitation of Expressed Message for Inducible Nitric Oxide Synthase445
- Chapter 41. Inducible Nitric Oxide Synthase Knockout Mouse and Low-Density Lipoprotein Oxidation452
- Chapter 42. Nitric Oxide and Resolution of Inflammation459
- Author Index467
- Subject Index501
Book details
- Vendor Elsevier S & T
- SKU 9780121822620
- ISBN-13 9780080497037
- Author Cadenas, Enrique
- Category Science
- Subject Research & Methodology
Do you have questions about this book?
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.
Key Features
* Presents information on nitrosothiols and nitric oxide in cell signaling
* Details nitric oxide and mitochondrial functions
* Explains nitric oxide synthases
Key Features
* Presents information on nitrosothiols and nitric oxide in cell signaling
* Details nitric oxide and mitochondrial functions
* Explains nitric oxide synthases
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