Nitric Oxide Synthase: Characterization and Functional Analysis: Characterization and Functional Analysis
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Table of contents
- Table of Contentsv
- Contributorsix
- Prefacexv
- Volumes in Seriesxvii
- Section I: Enzyme Activity Measurements, Nitric Oxide Detection, and Tissue Localization1
- Chapter 1. Electron Paramagnetic Resonance Detection of Nitric Oxide in Biological Samples3
- Chapter 2. Electrochemical and Spectroscopic Methods of Nitric Oxide Detection14
- Chapter 3. Detection of S-Nitrosothiols34
- Chapter 4. Nitrosation of Proteins41
- Chapter 5. Immunohistochemical Methods for Nitric Oxide Synthase47
- Chapter 6. NADPH Diaphorase Staining62
- Chapter 7. Immunohistochemical Visualization of Cyclic Nucleotides68
- Chapter 8. Detection of Downstream Components of Cyclic GMP Cascade by in Situ Hybridization Analysi80
- Chapter 9. Detection of Heme Oxygenase-1 and -2 Transcripts by Northern Blot and in Situ Hybridizati112
- Section II: Enzyme Expression, Purification, and Gene Regulation123
- Chapter 10. Purification of Endothelial Nitric Oxide Synthase125
- Chapter 11. Purification of Brain Nitric Oxide Synthase from Baculovirus Overexpression System and D130
- Chapter 12. Prokaryotic Expression, Purification, and Characterization of Intact and Modular Constru140
- Chapter 13. Generation of Monoclonal Antibodies to Endothelial Nitric Oxide Synthase152
- Chapter 14. Genetic Modulation of Inducible Nitric Oxide Synthase Expression156
- Chapter 15. Expression of Constitutive Brain Nitric Oxide Synthase and Inducible Nitric Oxide Syntha173
- Chapter 16. Structural Characterization of Human Neuronal Nitric Oxide Synthase Gene: Methodological184
- Chapter 17. Transcriptional Control of Endothelial Nitric Oxide Synthase Gene Expression197
- Chapter 18. Site-Directed Mutagenesis to Probe Endothelial Nitric Oxide Synthase Posttranslational M207
- Section III: Assessment of Nitric Oxide-Mediated Functions at Cell and Organ Levels213
- Chapter 19. Nitric Oxide and Endothelial Regulation of Vascular Tone215
- Chapter 20. Function of Nitric Oxide in Neuronal Cell Death228
- Chapter 21. Nitric Oxide–Cyclic Guanosine Monophosphate Pathway in Central Nervous System: In Vitr241
- Chapter 22. Detection and Contribution of Nitric Oxide to Endothelium Regulation of Microvascular To253
- Chapter 23. Regulation of Cerebral Circulation by Nitric Oxide264
- Chapter 24. Nitric Oxide in NAD/NADH-Dependent Protein Modification273
- Chapter 25. Assessment of Synaptic Effects of Nitric Oxide in Hippocampal Neurons282
- Chapter 26. Synaptic Transmission in Hippocampal Slice300
- Chapter 27. Redox Congeners of Nitric Oxide, N-Methyl-D-Aspartate Receptors, and Intracellular Calci309
- Chapter 28. Cytosolic and Nuclear Calcium Imaging by Confocal Microscopy319
- Chapter 29. Effects of Nitrogen Monoxide on Cellular Iron Metabolism329
- Index347
- Color Plate Section355
Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field.
Key Features
* Comprehensive protocols included for
* Detection of NO and related compounds by chemical, immunohistochemical, and in situ hybridization techniques
* Newly developed methods for the purification of neuronal and endothelial NO synthase, production of monoclonal antibodies to NO synthase, molecular cloning and expression of NO synthesis, and control of NO synthase gene expression
* Assessment of NO-mediated functions in neurons, central nervous system, cerebral circulation, synaptic transmission, and vascular tone
* Calcium imaging by confocal microscopy, evaluation of the effect of NO on iron metabolism, and detection of heme oxygenase-1 and -2 message level and distribution
Key Features
* Comprehensive protocols included for
* Detection of NO and related compounds by chemical, immunohistochemical, and in situ hybridization techniques
* Newly developed methods for the purification of neuronal and endothelial NO synthase, production of monoclonal antibodies to NO synthase, molecular cloning and expression of NO synthesis, and control of NO synthase gene expression
* Assessment of NO-mediated functions in neurons, central nervous system, cerebral circulation, synaptic transmission, and vascular tone
* Calcium imaging by confocal microscopy, evaluation of the effect of NO on iron metabolism, and detection of heme oxygenase-1 and -2 message level and distribution