Nuclear Structure and Gene Expression: Nuclear Matrix and Chromatin Structure
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Table of contents
- Contentsv
- Contributorsix
- Prefacexi
- Part I: Introduction1
- Chapter 1. The Nuclear Matrix: Past and Present3
- I. Introduction3
- II. The Nuclear Matrix: History and Definition6
- III. Ultrastructure of the Nuclear Matrix10
- IV. Core Filaments and Metabolic Centers of the Nuclear Matrix14
- V. Dense Bodies, Fluorescence Speckles, and Functional Domains of the Nucleus17
- VI. Nuclear Matrix and Cell Phenotype21
- VII. The Nuclear Matrix in Malignancy23
- VIII. Conclusion24
- References24
- Part II: Nuclear Matrix Structure and Function33
- Chapter 2. The Nuclear Matrix: Mapping Genomic Organization and Function in the Cell Nucleus35
- I. Introduction35
- II. In Situ Nuclear Matrix36
- III. Isolated Nuclear Matrix43
- IV. Nuclear Matrix Proteins53
- V. Nuclear Matrix Functions62
- VI. Nuclear Matrix and DNA Replication65
- VII. Concluding Remarks75
- References76
- Chapter 3. Composition and Structure of the Internal Nuclear Matrix87
- I. Scope and Introduction87
- II. The Nuclear Matrix: Definition and Confusion88
- III. Putative Structural Components of the Internal Nuclear Matrix92
- IV. Nuclear Matrix and Nuclear Function100
- V. Challenges and Outlook103
- References104
- Note Added in Proof110
- Chapter 4. High Unwinding Capability of Matrix Attachment Regions and ATC-Sequence Context-Specific111
- I. Introduction111
- II. Matrix Attachment Regions Continuously Base Unpaired Under Superhelical Strain112
- III. Determination of Core Unwinding Elements113
- IV. Unwinding Capability and MAR Activity118
- V. Cloning of cDNA Encoding MAR-Binding Protein SATB1124
- VI. SATB1 Recognition of Specific Sequence Context125
- VII. Unusual Mode of Binding by SATB1129
- VIII. Novel Motif Found in MAR-Binding Domain of SATB1131
- IX. Biological Significance of SATB1133
- X. Other MAR-Binding Proteins That Can Distinguish Wild-Type (25)7 from Mutated (24)8138
- XI. Conclusion and Future Prospects140
- References141
- Chapter 5. The Cyclin/Cyclin-Dependent Kinase (cdk) Complex: Regulation of Cell Cycle Progression an145
- I. Introduction145
- II. Mechanisms of Cyclin/cdk Function147
- III. Target Substrates of Cyclin/cdk152
- IV. Future Outlook162
- References163
- Part III: Chromatin Structure and Transcription Complexes175
- Chapter 6. Nuclear Architecture in Developmental Transcriptional Control of Cell Growth and Tissue-S177
- I. Introduction: Contributions of Nuclear Architecture to Transcriptional Control178
- II. Developmental Transcriptional Control during Proliferation and Differentiation: Regulation of Ce183
- III. Nuclear Structure Supporting Cell Cycle Stage-Specific Histone Gene Transcription in Proliferat191
- IV. Nuclear Structure Supporting Developmental and Steroid Hormone- Responsive Osteocalcin Gene Tran194
- V. Conclusions and Prospects202
- References204
- Chapter 7. Transcription by RNA Polymerase II and Nuclear Architecture215
- I. Introduction215
- II. Localization of RNA Polymerase II Transcription Sites in the Nucleus216
- III. Transcription and the Nuclear Matrix223
- IV. Transcription and Pre-mRNA Processing226
- V. Transcription and DNA Replication230
- VI. Outlook and Questions233
- References233
- Part IV: Nuclear mRNP Complexes241
- Chapter 8. Protein Deposition on Nascent Pre-mRNA Transcripts243
- I. Introduction243
- II. Primary Determinants for Deposition of Transcript-Binding Proteins246
- III. Timing of Protein Deposition on Nascent Pre-mRNA Transcripts262
- IV. Interdependence among Ribonucleoprotein Complexes on Pre-mRNA Transcripts270
- V. Interactions with Transcription Complexes272
- VI. Conclusions275
- References276
- Note Added in Proof295
- Index297
Nuclear Structure and Gene Expression assimilates the contributions of genome organization and of the components of the nuclear matrix to the control of DNA and RNA synthesis. Nuclear domains which accommodate DNA replication and gene expression are considered in relation to short-term developmental and homeostatic requirements as well as to long-term commitments to phenotypic gene expression in differentiated cells. Consideration is given to the involvement of nuclear structure in gene localization as well as to the targeting and concentration of transcription factors. Aberrations in nuclear architecture associated with and potentially functionally related to pathologies are evaluated. Tumor cells are described from the perspective of the striking modifications in both the composition and organization of nuclear components. Nuclear Structure and Gene Expression presents concepts as well as experimental approaches, which define functionality of nuclear morphology.
* Mechanisms of interaction between nuclear structure and genes
* Gene expression regulation by elements of the nuclear matrix
* How nuclear structure exerts a regulatory effect on other aspects of cell function/physiology
* Mechanisms of interaction between nuclear structure and genes
* Gene expression regulation by elements of the nuclear matrix
* How nuclear structure exerts a regulatory effect on other aspects of cell function/physiology