Elsevier S & T

PCR Strategies

Innis, Michael A.; Gelfand, David H.; Sninsky, John J.

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Table of contents
  • Cover
  • CONTENTSv
  • Contributorsix
  • Forewordxiii
  • Prefacexv
  • Part One: KEY CONCEPTS FOR PCR1
  • Chapter 1. The Use of Cosolvents to Enhance Amplification by the Polymerase Chain Reaction3
  • Chapter 2. DNA Polymerase Fidelity: Misinsertions and Mismatched Extensions17
  • Chapter 3. Extraction of Nucleic Acids: Sample Preparation from Paraffin-Embedded Tissues32
  • Chapter 4. Thermostable DNA Polymerases39
  • Chapter 5. Amplification of RNA: High-Temperature Reverse Transcription and DNA Amplification with T58
  • Chapter 6. Nucleic Acid Hybridization and Unconventional Bases69
  • Chapter 7. Practical Considerations for the Design of Quantitative PCR Assays84
  • Part Two: ANALYSIS OF PCR PRODUCTS109
  • Chapter 8. Carrier Detection of Cystic Fibrosis Mutations Using PCR-Amplified DNA and a Mismatch-Bin111
  • Chapter 9. Single-Stranded Conformational Polymorphisms121
  • Chapter 10. Analysis of PCR Products by Covalent Reverse Dot Blot Hybridization130
  • Chapter 11. High-Performance Liquid Chromatography Analysis of PCR Products140
  • Chapter 12. Heteroduplex Mobility Assays for Phylogenetic Analysis154
  • Chapter 13. PCR Amplification of VNTRs161
  • Part Three: RESEARCH APPLICATIONS177
  • Chapter 14. Site-Specific Mutagenesis Using the Polymerase Chain Reaction179
  • Chapter 15. Exact Quantification of DNA-RNA Copy Numbers by PCR-TGGE189
  • Chapter 16. The in Situ PCR: Amplication and Detection of DNA in a Cellular Context199
  • Chapter 17. Y Chromosome-Specific PCR: Maternal Blood213
  • Chapter 18. Genomic Subtraction220
  • Chapter 19. DNA Amplification-Restricted Transcription Translation (DARTT): Analysis of in Vitro and237
  • Chapter 20. DNA and RNA Fingerprinting Using Arbitrarily Primed PCR249
  • Chapter 21. PCR-Based Screening of Yeast Artificial Chromosome Libraries277
  • Chapter 22. Oligonucleotide Ligands That Discriminate between Theophylline and Caffeine289
  • Chapter 23. Generation of Single-Chain Antibody Fragments by PCR300
  • Chapter 24. Longer PCR Amplifications313
  • Chapter 25. Direct Analysis of Specific Bonds from Arbitrarily Primed PCR Reactions325
  • Part Four: ALTERNATIVE AMPLIFICATION STRATEGIES333
  • Chapter 26. Detection of Leber's Hereditary Optic Neuropathy by Nonradioactive Ligase Chain Reaction335
  • Chapter 27. Detection of Listeria monocytogenes by PCR-Coupled Ligase Chain Reaction347
  • Index363
Book details
  • Vendor Elsevier S & T
  • SKU 9780123721822
  • ISBN-13 9780080538549
  • Author Innis, Michael A.; Gelfand, David H.; Sninsky, John J.
  • Category Medical
  • Subject Genetics

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Description eBook delivery & access Reviews
PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR.
The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both!

Key Features
* Concepts explained
* Methods detailed
* Trouble-shooting emphasized
* Novel applications highlighted
* Major sections
* Key concepts for PCR
* Analysis of PCR products
* Research applications
* Alternative amplification strategies