Elsevier S & T

Peptide-Lipid Interactions

Simon, Sidney A.; McIntosh, Thomas J.

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Table of contents

Copyright Pageiv
Contentsv
Contributorsxiii
Prefacexvii
Previous Volumes in Seriesxix
Part I: Biophysical Techniques for Analyzing Peptide–Lipid Interactions1
Chapter 1. Peptide–Membrane Interactions Determined Using Site-Directed Spin Labeling3
I. Introduction4
II. Site-Directed Spin Labeling5
III. Membrane Interactions of Basic Peptides Derived from Cell-Signaling Motifs12
IV. Studies Directed at Channel-Forming and Other Membrane-Active Peptides18
V. Conclusions24
References25
Chapter 2. Isothermal Titration Calorimetry for Studying Interactions between Peptides and Lipid Mem31
I. Introduction to Isothermal Titration Calorimetry32
II. Measurement of the Reaction Enthalpy and the Binding Isotherm33
III. Binding Models for Analyzing Binding Isotherms37
IV. Complex Membrane-Binding/Membrane-Perturbation Equilibria41
V. Thermodynamics of the Membrane-Induced Coil–Helix Transition48
VI. Thermodynamics of Peptide Binding to Curved and Planar Membranes52
VII. Concluding Remarks54
References54
Chapter 3. Infrared Reflection–Absorption Spectroscopy of Lipids, Peptides, and Proteins in57
I. Introduction58
II. Experimental Approaches61
III. Information from IRRAS Measurements66
IV. IRRAS Applications to Lipid/Protein Langmuir Films75
V. Current State and Future Prospects for IRRAS83
References84
Chapter 4. Measuring the Depth of Amino Acid Residues in Membrane-Inserted Peptides by Fluorescence89
I. Introduction90
II. Determining Depth from Fluorescence Quenching by Empirical Inspection of Quenching Level90
III. Determining Depth by Parallax Analysis91
IV. Determining Depth by Distribution Analysis93
V. Comparing Parallax Analysis and Distribution Analysis97
VI. Investigating Membrane Protein Topography by Varying Fluorophore Depth Instead of Quencher Depth98
VII. A Cautionary Note Concerning Quenching Analysis98
VIII. Problems That Can Affect the Accuracy of Quenching Measurements99
IX. Studies of Membrane-Inserted Peptides by Fluorescence Quenching100
X. Conclusions111
References111
Chapter 5. Surface-Sensitive X-Ray and Neutron Scattering Characterization of Planar Lipid Model Mem117
I. Introduction118
II. Data Inversion and Modeling in Reflectivity Measurements122
III. Characterization of Planar Lipid Model Systems127
References150
Chapter 6. Lipid–Peptide Interaction Investigated by NMR163
I. Introduction164
II. Peptide Structure in the Membrane-Bound State165
III. Structure and Dynamics of the Lipid Matrix176
IV. Future Directions183
References184
Chapter 7. Peptide–Lipid Interactions in Supported Monolayers and Bilayers191
I. Peptide Insertion into Lipid Monolayers191
II. Supported Bilayers194
III. ATR-FTIR Spectroscopy of Peptides in Supported Bilayers195
IV. Analysis of Fusion Peptide–Lipid Interactions198
References201
Part II: Theoretical and Computational Analyses of Peptide-Lipid Interactions203
Chapter 8. Free Energy Determinants of Peptide Association with Lipid Bilayers205
I. Introduction206
II. Theory211
III. Mean-Field Studies of Peptide–Membrane Systems223
IV. Open Questions242
References244
Chapter 9. Investigating Ion Channels Using Computational Methods255
I. Introduction256
II. Understanding Channel Function from Structure257
III. Understanding Channel Function from Structural Homology266
IV. Future Prospects271
References271
Part III: Experimental Investigations of Peptide-Lipid Interactions275
Chapter 10. The Role of Electrostatic andNonpolar Interactions in the Association of Peripheral Prot277
I. Introduction278
II. Electrostatic and Hydrophobic Interactions Mediate the Membrane Association of Important Biologi279
III. The Membrane Interaction of Simple Basic Peptides: Theory and Experiment282
IV. The Combination of Experimental and Theoretical Approaches Provides a Detailed Comprehensive Pic289
V. Future Directions300
References303
Chapter 11. The Energetics of Peptide–Lipid Interactions: Modulation by Interfacial Dipoles and Ch309
I. Introduction310
II. Results318
III. Discussion327
IV. Conclusions332
References333
Chapter 12. Transmembrane α Helices339
I. Intrinsic Membrane Proteins339
II. Effects of Intrinsic Membrane Proteins on Lipid Bilayers345
III. Model Transmembrane α Helices351
IV. Biological Consequences of Hydrophobic Mismatch363
References365
Chapter 13. Lipidated Peptides as Tools for Understanding the Membrane Interactions of Lipid-Modifie371
I. Introduction372
II. Structures of Lipid Modifications of Proteins372
III. Chemistry of Peptide Lipidation375
IV. Thermodynamics of Association of Lipidated Peptides with Lipid Bilayers377
V. Kinetics of Dissociation of Lipidated Sequences from Bilayers: Protein Targeting by Kinetic Trapp383
VI. Partitioning of Lipidated Peptides into Liquid-Ordered Lipid Domains387
VII. Conclusion389
References390
Chapter 14. Experimental and Computational Studies of the Interactions of Amphipathic Peptides with397
I. Introduction398
II. Amphipathic α Helixes398
III. Amphipathic β Strands/Sheets415
References430
Chapter 15. Interactions of pH-Sensitive Peptides and Polymers with Lipid Bilayers: Binding and Memb437
I. Introduction438
II. Interaction of Viral Fusion Peptides with the Membrane440
III. Interaction of AcE4K with Lipid Bilayer Membranes (SOPC)442
IV. Effect of Peptide Binding on Membrane Stability450
V. Membrane Instability Induced by the pH-Sensitive Polymer Poly(2-Ethylacrylic Acid) (PEAA)456
VI. Conclusion460
References460
Chapter 16. The Hydrophobicity Threshold for Peptide Insertion into Membranes465
I. Introduction466
II. Results467
III. Discussion473
IV. Conclusions476
References477
Part IV: Specialized Topics of Biological Relevance481
Chapter 17. Signal Sequence Function in the Mammalian Endoplasmic Reticulum: A Biological Perspectiv483
I. Signal Sequences: Passage and Coronation484
II. Signal Peptides: A Profile for Function484
III. Signal Peptide Recognition: Where Biophysics Meets Biology487
IV. Yes, But What Happens at the Membrane?492
V. Where to from Here?494
VI. Conclusion496
References496
Chapter 18. The Process of Membrane Fusion: Nipples, Hemifusion, Pores, and Pore Growth501
I. Introduction502
II. Structural Features and Conformational Changes of Fusion Proteins503
III. Hemifusion and Its Possible Role in the Fusion Process506
IV. The Observed Temporal Sequence of Lipid Dye Spread and Fusion Pore Formation Depends upon Target508
V. The Dependence of Fusion on Spontaneous Curvature of Lipid Monolayers510
VI. An Ectodomain of HA Anchored to a Membrane Can Yield Fusion or End-State Hemifusion514
VII. Fusion Does Not Require a Precise Amino Acid Sequence of the TM Domain515
VIII. Capturing Candidates of Transitional Hemifusion515
IX. The Role of Fusion Proteins in Hemifusion and Pore Formation517
X. The Effects of Lipid Composition on Pore Flickering and Growth518
XI. Energy and Shape Considerations in Pore Growth522
XII. Outlook524
References524
Chapter 19. Prenylation of CaaX-Type Proteins: Basic Principles through Clinical Applications531
I. Introduction532
II. Enzymology of the CaaX Prenyltransferases: FTase and GGTase-I534
III. Development of FTase Inhibitors as Anticancer Agents540
IV. Clinical Results from Evaluation of FTIs as Antitumor Agents544
V. Summary and Conclusions545
References546
Chapter 20. Peptides as Probes of Protein–Protein Interactions Involved in Neurotransmitter Releas551
I. Introduction552
II. Peptide Design554
III. Characterization of Peptide Activity on Protein–Protein Interactions562
IV. Functional Analysis of Peptides in a Presynaptic Terminal564
V. Summary and Outlook567
References568
Index571

Book details

Vendor Elsevier S & T
SKU 9780121533526
ISBN-13 9780080490960
Author Simon, Sidney A.; McIntosh, Thomas J.
Category Medical
Subject Biochemistry

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This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion.

Key Features
* world-renowned experts
* state-of-the-art experimental methods
* monolayers, bilayers, biological membranes
* theoretical aspects and computer simulations
* rafts
* synaptic transmission
* membrane fusion
* signal transduction

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