Plant Tissue Culture: Theory and Practice: Theory and Practice

Bhojwani, S.S.; Razdan, M.K.

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Table of contents
  • Cover
  • Contentsvii
  • Prefacev
  • Chapter 1. Introductory history1
  • Chapter 2. Laboratory requirements and general techniques19
  • Chapter 3. Tissue culture media39
  • Chapter 4. Cell culture63
  • Chapter 5. Cellular totipotency95
  • Chapter 6. Somatic embryogenesis125
  • Chapter 7. Haploid production167
  • Chapter 8. Triploid production215
  • Chapter 9. Variant selection231
  • Chapter 10. In vitro pollination and fertilization269
  • Chapter 11. Zygotic embryo culture297
  • Chapter 12. Protoplast isolation and culture337
  • Chapter 13. Somatic hybridization and cybridization373
  • Chapter 14. Genetic engineering407
  • Chapter 15. Production of pathogen-free plants451
  • Chapter 16. Clonal propagation483
  • Chapter 17. Production of secondary metabolites537
  • Chapter 18. Germplasm storage563
  • Glossary of terms commonly used in plant tissue culture589
  • References603
  • Subject and plant index749
Book details
  • Vendor Elsevier S & T
  • SKU 9780444816238
  • ISBN-13 9780080539096
  • Author Bhojwani, S.S.; Razdan, M.K.
  • Category Science
  • Subject Biotechnology

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Since the publication of the first edition in 1983, several new and exciting developments have taken place in the field of plant tissue culture, which forms a major component of what is now called plant biotechnology. The revised edition presents updated information on theoretical, practical and applied aspects of plant tissue culture. Each chapter has been thoroughly revised and, as before, is written in lucid language, includes relevant media protocols, and is profusely illustrated with self-explanatory diagrams and original photographs. This book includes three new chapters: "Variant selection", "Genetic Engineering" and "Production of Industrial Compounds" and contains a complete bibliography and a glossary of terms commonly used in tissue culture literature.

This updated version proves to be an excellent text for undergraduate, postgraduate students and teachers in various fields of plant sciences and a useful reference book for those interested in the application of any aspect of this aseptic technology.