Platelet Protocols: Research and Clinical Laboratory Procedures
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Table of contents
- Contentsv
- Prefaceix
- Acknowledgmentsxi
- Chapter One. Basic Introduction to Platelets1
- Introduction2
- Platelets in Hemostasis3
- Membrane Surface Proteins6
- Events Associated with Platelet Aggregation9
- Platelet Agonists17
- Coagulation20
- Chapter Two. Laboratory Evaluation of Platelet Function27
- Introduction28
- Variables28
- Drawing and Processing Blood for Platelet Aggregation39
- Platelet Aggregation and Secretion43
- Platelet Agonists55
- Clot Retraction57
- Platelet Adhesion60
- Flow Cytometric Analysis of Platelet Surface Proteins61
- Receptor Occupancy63
- Chapter Three. Functional Abnormalities of Platelets69
- Introduction70
- Storage Pool Disorders (SPDs) and Release Defects71
- Chapter Four. Therapeutic Approaches to Inhibition of Platelet Function79
- Introduction80
- GPIIL–IIIa Antagonists80
- Other Anti-Platelet Therapies84
- Drugs that May Affect Platelet Function86
- Clinical Trials and Pharmacodynamic Measurementsof Platelet Inhibition89
- Appendix93
- Recipes for Citrate-Based Anticoagulants94
- Inhibitors Used in Platelet Techniques95
- Cytoskeletal Preparation from PRP95
- Cytoskeleton Preparations from Washed Platelets96
- Preparing Gel-Filtered Platelets97
- Preparing Washed Platelets99
- SDS Gel Electrophoresis102
- Gel Recipes103
- Western Blots (of Acrylamide Gels)109
- Staining of Platelets for Flow Cytometric Analysis112
- Sources of Aggregometers and Aggregation Reagents114
- Index115
- Color Plate Section117
Platelets are fragments of blood cells that occur in the blood of vertebrates and are associated with blood clotting. Scientists have made great strides in recent years in understanding what stimulates platelets to form blood clots at the molecular level and in developing drugs to inhibit platelet action. Their work has a direct effect on millions of people who deal with cardiovascular disease, strokes, surgery, physical trauma, and other conditions. While references to platelet function have been included in some large texts, there has not been a basic reference manual that researchers and clinicians can use in their daily work until now.
Platelet Protocols fills the need for a straightforward and comprehensive laboratory manual on current procedures for evaluating and analyzing platelet function and abnormalities. It is an easy-to-read, understandable resource which can be kept at the bench and referred to frequently by scientists, clinicians, and laboratory staff involved in platelet related areas. Topics range from the basics of anticoagulants to the latest developments in platelet testing.
Key Features
Includes:
* A basic introduction to platelet anatomy and physiology
* Testing procedures for new anti-platelet therapies
* Descriptions of platelet function abnormalities
* Therapeutic approaches to inhibition of platelet function
* Step-by-step methodologies with clear explanations
* Helpful appendixes of recipes, instructions, sources of reagents, and more
Platelet Protocols fills the need for a straightforward and comprehensive laboratory manual on current procedures for evaluating and analyzing platelet function and abnormalities. It is an easy-to-read, understandable resource which can be kept at the bench and referred to frequently by scientists, clinicians, and laboratory staff involved in platelet related areas. Topics range from the basics of anticoagulants to the latest developments in platelet testing.
Key Features
Includes:
* A basic introduction to platelet anatomy and physiology
* Testing procedures for new anti-platelet therapies
* Descriptions of platelet function abnormalities
* Therapeutic approaches to inhibition of platelet function
* Step-by-step methodologies with clear explanations
* Helpful appendixes of recipes, instructions, sources of reagents, and more