PROG NUCLEIC ACID RES&MOLECULAR BIO V3
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Table of contents
- Cover
- Contentsix
- List of Contributorsv
- Prefacevii
- Abbreviations and Symbolsviii
- Contents of Volume 1xii
- Contents of Volume 2xiii
- Contents of Volume 3xiv
- Articles Planned for Future Volumesxv
- Chapter 1. Isolation and Fractionation of Nucleic Acids1
- I. Introduction1
- II. Isolation of Deoxyribonucleic Acids2
- III. Separation of DNA from RNA7
- IV. Purification of DNA8
- V. Fractionation of DNA8
- VI. Soluble or Transfer RNA (sRNA)13
- VII. Ribosomal RNA16
- VIII. Virus RNA18
- IX. Purification of RNA19
- X. Fractionation of RNA20
- XI. Messenger RNA (Informational RNA)23
- XII. Conclusion27
- References28
- Chapter 2. Cellular Sites of RNA Synthesis33
- I. Introduction33
- II. Time Course of RNA Labeling in Intact Cells33
- III. RNA Migration and Nuclear Transplantation38
- IV. Intracellular Transfer of RNA during Mitosis38
- V. RNA Synthesis and Mitosis40
- VI. Enucleation of Cells and RNA Synthesis42
- VII. RNA Synthesis and Erythrocyte Formation45
- VIII. RNA Synthesis during DNA Synthesis46
- IX. Actinomycin D Inhibition of RNA Synthesis46
- X. Nucleolar Origin of Ribosomal RNA48
- XI. Site of sRNA Synthesis51
- XII. Intranuclear Shifts of RNA52
- XIII. Conclusion54
- References54
- Chapter 3. Ribonucleases in Taka-Diastase: Properties, Chemical Nature, and Applications59
- I. Introduction59
- II. Nomenclature60
- III. Preparation and Properties61
- IV. Structure and Function73
- V. Specificity81
- VI. Applications91
- References99
- Chapter 4. Chemical Effects of Ionizing Radiations on Nucleic Acids and Related Compounds103
- I. Physicochemical Basis104
- II. Pyrimidines110
- III. Purines120
- IV. Nucleosides and Nucleotides122
- V. Nucleic Acids133
- VI. Nucleoproteins138
- References142
- Chapter 5. The Regulation of RNA Synthesis in Bacteria145
- I. Introduction145
- II. Regulation of rRNA Synthesis147
- III. Regulation of sRNA Synthesis166
- IV. Regulation of mRNA Synthesis169
- V. A New Strategy for Studying Regulation171
- VI. Summary177
- Addendum178
- References179
- Chapter 6. Actinomycin and Nucleic Acid Function183
- I. Introduction184
- II. Molecular Basis of Action of Actinomycin185
- III. The Use of Actinomycin in the Study of Gene Action209
- IV. Summary229
- References230
- Chapter 7. De Novo Protein Synthesis in Vitro235
- I. Introduction236
- II. Definition of de Novo Synthesis238
- III. Mechanism of Formation of Polypeptide Chains241
- IV. Specific Protein Synthesis256
- V. Cell-Free Induction and Repression in Membrane Fractions from E. coli267
- VI. Biosynthesis of Alkaline Phosphate by Enzyme-Forming Particles (EFP) of E. coli285
- VII. Concluding Remarks Referencw288
- References290
- Chapter 8. Free Nucleotides in Animal Tissues299
- I. Introduction299
- II. History300
- III. The Metabolic Roles of Various Nucleotides300
- IV. Methods of Separation and Identification of Free Nucleotides304
- V. Distribution of Free Nucleotides in Tissues307
- VI. Changes in Free Nucleotide Pools in Various Physiological and Experimental Conditions313
- VII. Discussion and Conclusions322
- References323
- Index335
- Author Index335
- Subject Index358
PROG NUCLEIC ACID RES&MOLECULAR BIO V3