Elsevier S & T

Protein Engineering

Robertson, Dan; Noel, Joseph P.

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Table of contents

Table of Contentsv
Contributors to Volume 388ix
Prefacexiii
Volumes in Seriesxv
Section I: Methodology1
Chapter 1. Gene Site Saturation Mutagenesis: A Comprehensive Mutagenesis Approach3
Chapter 2. Single-Stranded DNA Family Shuffling10
Chapter 3. Module Shuffling21
Chapter 4. SCHEMA-Guided Protein Recombination34
Chapter 5. Staggered Extension Process In Vitro DNA Recombination41
Chapter 6. Using Incremental Truncation to Create Libraries of Hybrid Enzymes49
Chapter 7. Random Multirecombinant Polymerase Chain Reaction60
Chapter 8. Gene Library Synthesis by Structure-Based Combinatorial Protein Engineering74
Chapter 9. New Enzymes from Combinatorial Library Modules90
Chapter 10. Construction of Protein Fragment Complementation Libraries Using Incremental Truncation102
Section II: Applications: Optimization and Screening116
Chapter 11. GigaMatrix: A Novel Ultrahigh Throughput Protein Optimization and Discovery Platform119
Chapter 12. High Throughput Microplate Screens for Directed Protein Evolution134
Chapter 13. Periplasmic Expression as a Basis for Whole Cell Kinetic Screening of Unnatural Enzyme R145
Chapter 14. Engineering the Thermotolerance and pH Optimum of Family 11 Xylanases by Site-Directed M156
Chapter 15. Screening for Oxidative Resistance167
Chapter 16. Consensus-Based Engineering of Protein Stability: From Intrabodies to Thermostable Enzym176
Chapter 17. Improving the Functional Expression of N-Carbamoylase by Directed Evolution using the Gr187
Section III: Applications: Directed Evolution of Enzymatic Function197
Chapter 18. Directed Evolution of Lipases and Esterases199
Chapter 19. Protein Engineering of the Cytochrome P450 Monooxygenase from Bacillus megaterium208
Chapter 20. Directed Evolution of Aldolases224
Chapter 21. Changing the Enantioselectivity of Enzymes by Directed Evolution238
Chapter 22. Control of Stereoselectivity in Phosphotriesterase256
Section IV: Applications: Evolution of Biosynthetic Pathways267
Chapter 23. Manipulation and Analysis of Polyketide Synthases269
Chapter 24. Reactions Catalyzed by Mature and Recombinant Nonribosomal Peptide Synthetases293
Chapter 25. Engineering Carotenoid Biosynthetic Pathways315
Section V: Devices, Antibodies, and Vaccines331
Chapter 26. Directed Evolution of Bacteriorhodopsin for Device Applications333
Chapter 27. Engineering Antibody Affinity by Yeast Surface Display348
Chapter 28. A Conditionally Replicating Virus as a Novel Approach Toward an HIV Vaccine359
Author Index381
Subject Index405

Book details

Vendor Elsevier S & T
SKU 9780121827939
ISBN-13 9780080497259
Author Robertson, Dan; Noel, Joseph P.
Category Science
Subject Biochemistry

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This MIE volume covers methods for a multitude of topics among which are computational methods, laboratory methods, enzyme optimization, binding proteins/antibodies, and screening technologies.

Table of Contents
-Methodology
-Applications-Opzimization and Screening
-Applications-Directed Evolution of Enzymatic Function
-Applications-Evolution of Biosynthetic Pathways
-Devices, Antibodies and Vaccines

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