Elsevier S & T

Protein Sensors of Reactive Oxygen Species, Part A: Selenoproteins and Thioredoxin: Selenoproteins and Thioredoxin

Packer, Lester

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 347ix
  • Prefacexv
  • Volume in Seriesxvii
  • Section I: Selenoproteins1
  • Chapter 1. Selenoprotein Biosynthesis: Purification and Assay of Components Involved in Selenocystei3
  • Chapter 2. Selenocysteine Insertion Sequence Element Characterization and Selenoprotein Expression17
  • Chapter 3. Transfer RNAs That Insert Selenocysteine24
  • Chapter 4. Purification and Analysis of Selenocysteine Insertion Sequence-Binding Protein 240
  • Chapter 5. Nonsense-Mediated Decay: Assaying for Effects on Selenoprotein mRNAs49
  • Chapter 6. Novel Selenoproteins Identified from Genomic Sequence Data57
  • Chapter 7. Semisynthesis of Proteins Containing Selenocysteine70
  • Chapter 8. Mammalian Selenoprotein Gene Signature: Identification and Functional Analysis of Selenop84
  • Chapter 9. Estimation of Individual Types of Glutathione Peroxidases101
  • Chapter 10. High-Throughput 96-Well Microplate Assays for Determining Specific Activities of Glutath113
  • Chapter 11. Selenoprotein P121
  • Chapter 12. Iodothyronine Deiodinases125
  • Chapter 13. Expression and Regulation of Thioredoxin Reductases and Other Selenoproteins in Bone168
  • Chapter 14. Selenoprotein W179
  • Chapter 15. Genetic and Functional Analysis of Mammalian Sep15 Selenoprotein187
  • Chapter 16. Selenocysteine Lyase from Mouse Liver198
  • Chapter 17. Selenocysteine Methyltransferase203
  • Chapter 18. Phospholipid–Hydroperoxide Glutathione Peroxidase in Sperm208
  • Chapter 19. In Vivo Antioxidant Role of Glutathione Peroxidase: Evidence from Knockout Mice213
  • Chapter 20. Recombinant Expression of Mammalian Selenocysteine- Containing Thioredoxin Reductase and226
  • Chapter 21. Mammalian Thioredoxin Reductases as Hydroperoxide Reductases236
  • Chapter 22. Tryparedoxin and Tryparedoxin Peroxidase244
  • Chapter 23. Trypanothione and Tryparedoxin in Ribonucleotide Reduction259
  • Chapter 24. Selenium- and Vitamin E-Dependent Gene Expression in Rats: Analysis of Differentially Ex267
  • Section II: Thioredoxin277
  • Chapter 25. Overview279
  • Chapter 26. Thioredoxin and Glutaredoxin Isoforms286
  • Chapter 27. Mammalian Thioredoxin Reductases297
  • Chapter 28. Mitochondrial Thioredoxin Reductase and Thiol Status307
  • Chapter 29. Protein Electrophoretic Mobility Shift Assay to Monitor Redox State of Thioredoxin in Ce317
  • Chapter 30. Recycling of Vitamin C by Mammalian Thioredoxin Reductase327
  • Chapter 31. Thioredoxin Cytokine Action332
  • Chapter 32. Identification of Thioredoxin-Linked Proteins by Fluorescence Labeling Combined with Iso339
  • Chapter 33. Thioredoxin and Mechanism of Inflammatory Response349
  • Chapter 34. Redox State of Cytoplasmic Thioredoxin360
  • Chapter 35. Thioredoxin, Thioredoxin Reductase, and Thioredoxin Peroxidase of Malaria Parasite Plasm370
  • Chapter 36. Human Placenta Thioredoxin Reductase: Preparation and Inhibitor Studies382
  • Chapter 37. Classification of Plant Thioredoxins by Sequence Similarity and Intron Position394
  • Chapter 38. Ferredoxin-Dependent Thioredoxin Reductase: A Unique Iron–Sulfur Protein403
  • Chapter 39. Plant Thioredoxin Gene Expression: Control by Light, Circadian Clock, and Heavy Metals412
  • Chapter 40. Thioredoxin Genes in Lens: Regulation by Oxidative Stress421
  • Chapter 41. Thioredoxin Overexpression in Transgenic Mice436
  • Chapter 42. Multiplex Reverse Transcription-Polymerase Chain Reaction for Determining Transcriptiona441
  • Chapter 43. Redox Regulation of Cell Signaling by Thioredoxin Reductases451
  • Author Index463
  • Subject Index497
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822484
  • ISBN-13 9780080496955
  • Author Packer, Lester
  • Category Science
  • Subject Research & Methodology

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This volume of Methods in Enzymology is concerned with the rapidly developing field of selenoprotein synthesis and its related molecular genetics. Progressive information on the topics of proteins as redox sensors, selenoproteins, and the thioredoxin system is studied using methods such as bioinformatics, DNA chip technology, cell biology, molecular genetics, and enzymology. The information on novel selenoproteins identified from genomic sequence data, as well as current knowledge on glutathione peroxidases, selenoprotein P, iodothyronine deiodinases, and thioredoxin reductases, is presented in a method-based approach.