Elsevier S & T

Recognition of Carbohydrates in Biological Systems, Part A : General Procedures: General Procedures

Lee, Y. C.; Lee, Reiko T.

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Table of contents

Table of Contentsv
Contributors to Volumeix
Prefacexv
Volumes in Seriesxvii
Section I: Preparative Methods1
Chapter 1. Transition Metal-Catalyzed Cross-Coupling Reactions toward the Synthesis of a-d- Mannopy3
Chapter 2. Ruthenium Carbenoids as Catalysts for Olefin Metathesis of w-Alkenyl Glycosides17
Chapter 3. Digoxin Derivatives as Tools for Glycobiology29
Chapter 4. Synthesis of Peptide-Based Trivalent Scaffold for Preparation of Cluster Glycosides38
Chapter 5. Chemoenzymatic Synthesis of Diverse Asparagine-Linked Oligosaccharides44
Chapter 6. Enzymatic Synthesis of Neoglycoconjugates by Transglycosylation with Endo-B-N-acetylgluco64
Chapter 7. Chemoenzymatic Synthesis of Neoglycopeptides Using Endo b-N-acetylglucosaminidase from Mu74
Chapter 8. Preparative-Scale Chemoenzymatic Synthesis of Large Carbohydrate Assemblies Using a(1„>86
Chapter 9. Synthesis of Galactose-Containing Oligosaccharides through Superbeads and Superbug Approc106
Chapter 10. Conjugating Low Molecular Mass Carbohydrates to Proteins 1. Monitoring the Progress of C125
Chapter 11. Conjugating Low Molecular Mass Carbohydrates to Proteins 2. Recovery of Excess Ligand Us140
Chapter 12. Neoglycolipid Technology: Deciphering Information Content of Glycome160
Chapter 13. Preparation and Utility of Neoproteoglycan Probes in Analyses of Interaction with Glycos196
Chapter 14. Modular Sunthesis and Study of Multivalent Carbohydrate Ligands with Long and Flexible L209
Chapter 15. Sugar Arrays in Microtiter Plates218
Chapter 16. Comparative Studies of Galactoside- Containing Clusters226
Chapter 17. Multivalent Breast Cancer T-Antigen Markers Scaffolded onto PAMAM Dendrimers240
Chapter 18. Constructing Azide-Labeled Cell Surfaces Using Polysaccharide Biosynthetic Pathways249
Chapter 19. Cell Wall Engineering of Living Bacteria through Biosynthesis273
Chapter 20. Molecular Biological Fingerprinting of Human Lectin Expression by RT-PCR287
Section II: General Techniques299
Chapter 21. Visualization and Characterization of Receptor Clusters by Transmission Electron Microsc301
Chapter 22. Exploring Kinetics and Mechanism of Protein- Sugar Recognition by Surface Plasmon Resona312
Chapter 23. Quantitative Lectin-Carbohydrate Interaction Analysis on Solid-Phase Surfaces Using Bios330
Chapter 24. Surface Plasmon Resonance Study of RNA–Aminoglycoside Interactions340
Chapter 25. Frontal Affinity Chromatography as a Tool for Elucidation of Sugar Recognition Propertie353
Chapter 26. Evaluating Carbohydrate-Protein Binding Interactions Using Frontal Affinity Chromatograp369
Chapter 27. Determination of Protein-Oligosaccharide Binding by Nanoelectrospray Fourier- Transform376
Chapter 28. Analysis of Lectin-Carbohydrate Interactions by Capillary Affinophoresis398
Chapter 29. Describing Topology of Bound Ligand by Transferred Nuclear Overhausers Effect Spectrosco417
Chapter 30. Studies of Carbohydrate-Protein Interactions by Capillary Electrophoresis434
Chapter 31. Carbohydrate-Lectin Cross-Linking Interactions: Structural, Thermodynamic, and Biologica455
Chapter 32. Calorimetric Evaluation of Protein- Carbohydrate Affinities486
Chapter 33. Fluorescence Polarization to Study Galectin- Legend Interactions504
Chapter 34. Lectin from Bulbs of Crocus sativus Recognizing N-Linked Core Glycan: Isolation and Bind512
Chapter 35. Direct Fluorescence-Based Detection Methods for Multivalent Interactions523
Chapter 36. Ultrasensitive Analysis of Sializ Acids and Oligo/Polysialic Acids by Fluorometric High-543
Chapter 37. Identification of Lectins from Genomic Sequence Data560
Chapter 38. Exploring Enzyme Amplification to Characterize Specificities of Protein- Carbohydrate Re567
Author Index585
Subject Index613
Color Plate SectionColor Plate-1

Book details

Vendor Elsevier S & T
SKU 9780121822651
ISBN-13 9780080497051
Author Lee, Y. C.; Lee, Reiko T.
Category Medical
Subject Pharmacology

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Recognition of carbohydrates in biological systems has been gaining more and more attention in recent years. Although methodology for studying recognition has been developing, there is no volume that covers the wide area of methodology of carbohydrate recognition. This volume and its companion, Volume 363, present state-of-the-art methodologies, as well as the most recent biological observations in this area.

* Covers the isolation/synthesis of substances used in studying interactions involving carbohydrates
* Discussed the methodology for measuring such interactions
* Biological roles for such interactions are also covered

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