Elsevier S & T

Recognition of Carbohydrates in Biological Systems, Part B: Specific Applications: Specific Applications

Lee, Y. C.

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Table of contents

Table of Contentsv
Contributors to Volume 363ix
Prefacexv
Volumes in Seriesxvii
Section I: Carbohydrate-Binding Proteins1
Chapter 1. Structure–Function Analysis of C-Type Animal Lectins3
Chapter 2. Preparation of Recombinant Mannan-Binding Protein with a Native Oligomeric Structure16
Chapter 3. Antitumor Activity of Mannan–Binding Protein26
Chapter 4. Carbohydrate Binding Activity of Annexin V toward a Bisecting N-Acetylglucosamine34
Chapter 5. Recombinant Plant Lectins and Their Mutants47
Chapter 6. Psathyrella velutina Lectin as a Specific Probe for N-Acetylneuraminic Acid in Glycoconju77
Chapter 7. Probing the Binding Specificity of C-Type Lectins in Vivo90
Chapter 8. Application of a Multivalent Glycoprobe: Characterization of Sugar-Binding Specificity of104
Chapter 9. Probing for Masked and Unmasked Siglecs on Cell Surfaces113
Chapter 10. The Man/GalNAc-4-SO4-Receptor: Relating Specificity to Function121
Chapter 11. Bacterium–Host Protein–Carbohydrate Interactions134
Chapter 12. Design and Synthesis of Galectin Inhibitors157
Section II: Glycoproteins and Glycolipids171
Chapter 13. RNA Interference: Analyzing the Function of Glycoproteins and Glycosylating Proteins in173
Chapter 14. Interaction of Glycosphingolipids with Signal Transducers and Membrane Proteins in Glyco191
Chapter 15. Interaction of GM2 Activator Protein with Sulfated and Sialylated Glycosphinogolipids207
Chapter 16. Differences in Recognition of Sulfated and Sialylated Carbohydrate Chains222
Chapter 17. Interaction of GM2 Activator Protein with Glycosphinogolipids230
Chapter 18. Structural Basis for the Enzymatic Resistance of the GM2 Ganglioside242
Chapter 19. Lipid Modulation of Glycosphingolipid (GSL) Receptors: Soluble GSL Mimics Provide New Pr264
Chapter 20. Glycosphingolipid-Induced Cell Signaling: Apoptosis284
Chapter 21. Roles of Glycosphingolipids in Cell Signaling: Adhesion, Migration, and Proliferation300
Chapter 22. Thin-Layer Chromatography; Immunostaining of Glycolipid Antigens; and Interpretation of312
Chapter 23. Complex Gangliosides as Cell Surface Inhibitors for the Ecto-NAD+ Glycohydrolase of CD38319
Section III: Polysaccharides325
Chapter 24. Nitrocellulose Filter Binding to Assess Binding of Glycosaminoglycans to Proteins327
Chapter 25. Characterization of Polysaccharide Conformational Epitopes by Surface Plasmon Resonance340
Chapter 26. Study of Hyaluronan-Binding Proteins and Receptors Using Iodinated Hyaluronan Derivative354
Chapter 27. Recognition of Glycosaminoglycans by Midkine365
Chapter 28. Control of Leishmania–Sand Fly Interactions by Polymorphisms in Lipophosphoglycan Stru377
Chapter 29. Use of Iodinated Hyaluronan Derivatives to Study Hyaluronan Binding, Endocytosis, and Me382
Section IV: Enzymes and Cells393
Chapter 30. Carbohydrates as Inhibitors of Rotaviral Infection395
Chapter 31. Cell-Based Screening of Active-Site Specific Chaperone for the Treatment of Fabry Diseas412
Chapter 32. Glycosylamidines as Potent Selective and Easily Accessible Glycosidase Inhibitors and Th421
Chapter 33. Diglycoside-Specific Glycosidases444
Chapter 34. Use of Synthetic Oligosaccharide Substrate Analogs to Map the Active Sites of N-Acetylgl459
Chapter 35. Recombinant Ganglioside GM2 Synthase Expression in Insect Cells and Enzyme Assay476
Chapter 36. Recombinant Influenza C Hemagglutinin- Esterase as a Probe for Sialic Acid 9-OAcetylatio489
Chapter 37. Preparation of Recombinant Human Galectin-1 and Use in T-Cell Death Assays499
Chapter 38. Carbohydrate Recognition of Interleukin-2 in Cell Proliferation518
Chapter 39. Carbohydrate Recognition of Vesicular Integral Protein of 36 kDa (VIP36) in Intracellula525
Chapter 40. Functions of Galectins in Cell Adhesion and Chemotaxis532
Chapter 41. Physical, Chemical, Antigenic, and Immunologic Characterization of Polygalacturonan, Its552
Author Index569
Subject Index611

Book details

Vendor Elsevier S & T
SKU 9780121822668
ISBN-13 9780080497068
Author Lee, Y. C.
Category Science
Subject Biochemistry

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Recognition of carbohydrates in biological systems has been gaining more and more attention in recent years. Although methodology for studying recognition has been developing, there is no volume that covers the wide area of methodology of carbohydrate recognition. This volume and its companion, Volume 362, present state-of-the-art methodologies, as well as the most recent biological observations in this area.

* Covers carbohydrate-binding proteins
* Discusses glycoproteins and glycolipids
* Polysaccharides, enzymes and cells are also covered

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