Redox Cell Biology and Genetics, Part A

Sen, Chandan K.; Packer, Lester

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 352ix
  • Prefacexv
  • Volumes in Seriesxvii
  • Section I: Cellular Responses1
  • Chapter 1. Measurement of Absolute Oxygen Levels in Cells and Tissues Using Oxygen Sensors and 2-Nit3
  • Chapter 2. Detection of Oxygen-Sensing Properties of Mitochondria31
  • Chapter 3. Direct Detection of Singlet Oxygen via Its Phosphorescence from Cellular and Fungal Cultu41
  • Chapter 4. Molecular Analysis of Mitogen-Activated Protein Kinase Signaling Pathways Induced by Reac53
  • Chapter 5. Detection of Intracellular Reactive Oxygen Species in Cultured Cells Using Fluorescent Pr62
  • Chapter 6. Flow Cytometric Determination of Cytoplasmic Oxidants and Mitochondrial Membrane Potentia71
  • Chapter 7. Flow Cytometric Determination of Lipid Peroxidation Using Fluoresceinated Phosphoethanola80
  • Chapter 8. Determination of Intracellular Reactive Oxygen Species as Function of Cell Density91
  • Chapter 9. Identification of Redox-Active Proteins on Cell Surface101
  • Chapter 10. Probing Redox Activity of Human Breast Cells by Scanning Electrochemical Microscopy112
  • Chapter 11. Determining Influence of Oxidants on Nuclear Transport Using Digitonin-Permeabilized Cel123
  • Chapter 12. Functional Imaging of Mitochondrial Redox State135
  • Chapter 13. Hydrogen Peroxide-Induced Apoptosis: Oxidative or Reductive Stress?150
  • Chapter 14. Peroxidation of Phosphatidylserine in Mechanisms of Apoptotic Signaling159
  • Chapter 15. Quantitative High Throughput Endothelial Cell Migration and Invasion Assay System175
  • Chapter 16. In Vitro Model of Oxidative Stress in Cortical Neurons183
  • Chapter 17. Glutamate-Induced c-Src Activation in Neuronal Cells191
  • Chapter 18. Measurement of Inflammatory Properties of Fatty Acids in Human Endothelial Cells198
  • Chapter 19. Redox Control of Tissue Factor Expression in Smooth Muscle Cells and Other Vascular Cell220
  • Chapter 20. Redox Processes Regulate Intestinal Lamina Propria T Lymphocytes232
  • Chapter 21. Linker for Activation of T Cells: Sensing Redox Imbalance248
  • Chapter 22. Generation of Prooxidant Conditions in Intact Cells to Induce Modifications of Cell Cycl258
  • Chapter 23. Analysis of Transmembrane Redox Reactions: Interaction of Intra- and Extracellular Ascor268
  • CH4Chapter 24. Regulation of Endothelial Cell Proliferation by Nitric Oxide280
  • Chapter 25. Fluorescent Imaging of Mitochondrial Nitric Oxide in Living Cells296
  • Section II: Tissues and Organs305
  • Chapter 26. Detection of Reactive Oxygen and Nitrogen Species in Tissues Using Redox-Sensitive Fluor307
  • Chapter 27. Simultaneous Detection of Tocopherols and Tocotrienols in Biological Samples Using HPLCC326
  • Chapter 28. In Vivo Measurement of Oxidative Stress Status in Human Skin333
  • Chapter 29. Localization of Oxidation-Specific Epitopes in Tissue340
  • Chapter 30. Quantitation of S-Nitrosothiols in Cells and Biological Fluids347
  • Chapter 31. Peroxisomal Fatty Acid Oxidation and Cellular Redox361
  • Chapter 32. Ultrastructural Localization and Relative Quantification of 4-Hydroxynonenal-Modified Pr373
  • Chapter 33. Ultrastructural Localization of Light-Induced Lipid Peroxides378
  • Chapter 34. A Survival Model for Study of Myocardial Angiogenesis391
  • Chapter 35. Determination of Angiogenesis-Regulating Properties of NO407
  • Chapter 36. Hemangioma Model for in Vivo Angiogenesis: Inducible Oxidative Stress and MCP-1Expressio422
  • Chapter 37. Redox Aspects of Vascular Response to Injury432
  • Chapter 38. Involvement of Superoxide in Pathogenic Action of Mutations That Cause Alzheimer’s Dis455
  • Chapter 39. Three-Dimensional Redox Imaging of Frozen- Quenched Brain and Other Organs475
  • Chapter 40. In Vivo Fluorescent Imaging of NADH Redox State in Brain482
  • Chapter 41. Nitroxyl Probes for Brain Research and Their Application to Brain Imaging494
  • Chapter 42. Analytical Implications of Iron Dithiocarbamates for Measurement of Nitric Oxide506
  • Author Index523
  • Subject Index561
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822552
  • ISBN-13 9780080496993
  • Author Sen, Chandan K.; Packer, Lester
  • Category Science
  • Subject Research & Methodology

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.

Key Features
* Cellular Responses
* Tissues and Organs