Elsevier S & T

Regulators and Effectors of Small GTPases, Part D: Rho Family: Rho Family

Abelson, John N.; Simon, Melvin I.

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 325ix
  • Prefacexv
  • Volumes in Seriesxvii
  • Section I: Purification, Posttranslational Modification, and in Vitro Regulation1
  • Chapter 1. Purification and Biochemical Characterization of TC103
  • Chapter 2. Expression and Purification of Rho/RhoGDI Com- plexes15
  • Chapter 3. Bacterial Expressed DH and DH/PH Domains25
  • Chapter 4. Biochemical Analysis of Regulation of Vav, a Guanine-Nucleotide Exchange Factor for Rho F38
  • Chapter 5. Activation of Rac1 by Human Tiam151
  • Chapter 6. Activation of Rho GEF Activity by Gα1361
  • Chapter 7. Purification and Characterization of Guanine Nucleotide Dissociation Stimulator Protein71
  • Chapter 8. Purification and Biochemical Activity of Salmonella Exchange Factor SopE82
  • Chapter 9. Stimulation of Rho GDI Release by ERM Proteins91
  • Chapter 10. Expression and Activity of Human Prenylcysteine- Directed Carboxyl Methyltransferase101
  • Chapter 11. Purification and Evaluation of Large Clostridial Cytotoxins That Inhibit Small GTPases o114
  • Chapter 12. Rho GTPase-Activating Toxins: Cytotoxic Necrotizing Factors and Dermonecrotic Toxin125
  • Section II: Purification and Activity of GTPase Targets137
  • Chapter 13. Measurement of GTPase ∙ Effector Affinities139
  • Chapter 14. Purification and in Vitro Activity of Rho-Associated Kinase149
  • Chapter 15. Purification and in Vitro Activities of p21-Activated Kinases155
  • Chapter 16. Stimulation of Phospholipase C-β2 by Rho GTPases167
  • Chapter 17. Regulation of Phospholipase D1 Activity by Rho GTPases177
  • Chapter 18. In Vitro Interaction of Phosphoinositide-4-phosphate 5-Kinases with Rac190
  • Chapter 19. Activity and Regulation of p35/Cdk5 Kinase Complex200
  • Chapter 20. Actin Assembly Mediated by Arp2/3 Complex and WASP Family Proteins214
  • Chapter 21. In Vitro Actin Polymerization Using Polymorphonuclear Leukocyte Extracts237
  • Section III: Analysis of Rho GTPase Function255
  • Chapter 22. Determination of GTP Loading on Rac and Cdc42 in Platelets and Fibroblasts257
  • Chapter 23. Determination of GTP Loading on Rho264
  • Chapter 24. Use and Properties of ROCK-Specific Inhibitor Y-27632273
  • Chapter 25. Inducible Membrane Recruitment of Small GTP-Binding Proteins by Rapamycin-Based System i285
  • Chapter 26. Expression of Rho GTPases Using Retroviral Vectors295
  • Chapter 27. Expression of Rho GTPases Using Adenovirus Vectors303
  • Chapter 28. In Vivo Activity of Wild-Type and Mutant PAKs315
  • Chapter 29. Single Cell Assays for Rac Activity327
  • Chapter 30. Effect of Rho GTPases on Na–H Exchanger in Mammalian Cells334
  • Chapter 31. Actin Filament Assembly in Permeabilized Platelets349
  • Chapter 32. Rho GTPases: Secretion and Actin Dynamics in Permeabilized Mast Cells356
  • Chapter 33. Isolation and in Vitro Contraction of Stress Fibers369
  • Chapter 34. Inhibition of Rho GTPases Using Protein Geranylgeranyltransferase I Inhibitors381
  • Chapter 35. Imaging Spatiotemporal Dynamics of Rac Activation in Vivo with FLAIR389
  • Section IV: Biological Assays of Rho GTPase Function401
  • Chapter 36. Rho-like GTPases in Tumor Cell Invasion403
  • Chapter 37. Mouse Embryo Fibroblasts. A Genetic Model System for Studying Rho- and Ras-Dependent Cel415
  • Chapter 38. Analyses of Transforming Activity of Rho Family Activators425
  • Chapter 39. Rho GTPases and Cell Migration–Fibroblast Wound Healing441
  • Chapter 40. Rho GTPases and Cell Migration: Measurement of Macrophage Chemotaxis449
  • Chapter 41. Rho GTPases and Macrophage Phagocytosis462
  • Chapter 42. Rho GTPases and Axonal Growth Cone Collapse473
  • Chapter 43. Study of in Situ Function of Cytoskeletal Proteins in Lamellipodia and Filopodia Using M482
  • Chapter 44. Modulation of Actin Cytoskeleton by Salmonella GTPase Activating Protein SptP496
  • Author Index505
  • Subject Index531
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822262
  • ISBN-13 9780080496801
  • Author Abelson, John N.; Simon, Melvin I.
  • Category Science
  • Subject Biochemistry

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This volume and its companions (Volumes 255, 256, 257, and the forthcoming 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.