Elsevier S & T

Regulators and Effectors of Small GTPases, Part E: GTPases Involved in Vesicular Traffic: GTPases Involved in Vesicular Traffic

Abelson, John N.

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 329xi
  • Prefacexvii
  • Volumes in Seriesxix
  • Section I: Rab GTPases1
  • Chapter 1. Structural Basis for Rab Function: An Overview3
  • Chapter 2. Expression of Wild-Type and Mutant Green Fluorescent Protein–Rab1 for Fluorescence Micr6
  • Chapter 3. Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Es14
  • Chapter 4. Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases31
  • Chapter 5. Antibody and Oligonucleotide Probes to Distin- guish Intracellular Expression and Localiz39
  • Chapter 6. Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Protein50
  • Chapter 7. Purification and Properties of Rab3 GDP/GTP Exchange Protein59
  • Chapter 8. Purification and Properties of Rab3 GTPase-Activating Protein67
  • Chapter 9. Rabphilin-3: A Target Molecule for Rab3 Small G Proteins75
  • Chapter 10. Doc2α as Modulator of Ca2+ -Dependent Exocytosis83
  • Chapter 11. Purification and Properties of Sec4p GTPase-Activating Protein91
  • Chapter 12. Purification and Characterization of Yeast Exocyst Complex100
  • Chapter 13. Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling111
  • Chapter 14. Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Ac120
  • Chapter 15 Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex132
  • Chapter 16. Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis145
  • Chapter 17. Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their I157
  • Chapter 18. Expression and Functional Analyses of Rab8 and Rab11a in Exocytic Transport from trans-G165
  • Chapter 19. Expression and Properties of Rab7 in Endosome Function175
  • Chapter 20. Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Org188
  • Chapter 21. Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase δ Subunit197
  • Chapter 22. Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial C210
  • Chapter 23. Expression and Properties of Rab25 in Polarized Madin–Darby Canine Kidney Cells225
  • Chapter 24. Purification of TRAPPfrom Saccharomyces cerevisiae and Identification of Its Mammalian C234
  • Section II: ADP-Ribosylation Factor (ARF) GTPases243
  • Chapter 25. Structural and Functional Organization of ADP- Ribosylation Factor (ARF) Proteins245
  • Chapter 26. Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathway247
  • Chapter 27. Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation F256
  • Chapter 28. Expression, Purification, and Measurements of Activity of ARNO1, a Guanine Nucleotide Ex264
  • Chapter 29. Expression, Purification, and Biochemical Properties of EFA6, a Sec7 Domain-Containing G272
  • Chapter 30. Isolation and Properties of GRP1, an ADP-Ribosylation Factor (ARF)–Guanine Nucleotide279
  • Chapter 31. Functional Analysis of ADP-Ribosylation Factor (ARF) Guanine Nucleotide Exchange Factors290
  • Chapter 32. Isolation, Cloning, and Characterization of Brefeldin A-Inhibited Guanine Nucleotide-Exc300
  • Chapter 33. Expression, Purification, and Properties of ADP-Ribosylation Factor (ARF) GAP1307
  • Chapter 34. Expression, Analysis, and Properties of Yeast ADP- Ribosylation Factor (ARF) GTPase Acti317
  • Chapter 35. Purification and Properties of ARD1, an ADP- Ribosylation Factor (ARF)-Related Protein w324
  • Chapter 36. Purification and Characterization of GIT Family of ADP-Ribosylation Factor (ARF) GTPase-335
  • Chapter 37. Assay and Purification of Phosphoinositide-Depen- dent ADP-Ribosylation Factor (ARF) GTP343
  • Chapter 38. Biological Properties and Measurement of Phos- pholipase D Activation by ADP-Ribosylatio355
  • Chapter 39. Use of Aminoglycoside Antibiotics and Related Compounds to Study ADP-Ribosylation Factor372
  • Chapter 40. Adaptor Protein 1-Dependent Clathrin Coat Assembly on Synthetic Liposomes and Golgi Memb379
  • Chapter 41. Receptor-Dependent Formation of COPI-Coated Vesicles from Chemically Defined Donor Lipos388
  • Chapter 42. ADP-Ribosylation Factor (ARF) as Regulator of Spectrin Assembly at Golgi Complex405
  • Chapter 43. Purification, Properties, and Analysis of yARL3417
  • Chapter 44. Preparation and Assay of Recombinant ADP-Ribosylation Factor-Like Protein-1 (ARL1)424
  • Section III: Sar GTPases429
  • Chapter 45. Purification and Properties of Rat Liver Sec23–Sec24 Complex431
  • Chapter 46. Purification of Functional Sec13p–Sec31p Complex, A Subunit of COPII Coat438
  • Section IV: Dynamin GTPases445
  • Chapter 47. Expression, Purification, and Functional Assays for Self-Association of Dynamin-1447
  • Chapter 48. Analysis of Phosphoinositide Binding by Pleckstrin Homology Domain from Dynamin457
  • Chapter 49. Mapping Dynamin Interdomain Interactions with Yeast Two-Hybrid and Glutathione S-Transfe468
  • Chapter 50. Interactions of Dynamin and Amphiphysin with Liposomes478
  • Chapter 51. Activation of Dynamin GTPase Activity by Phosphoinositides and SH3 Domain-Containing Pro486
  • Section V: Septin GTPases497
  • Chapter 52. Expression and Analysis of Properties of Septin CDCrel-1 in Exocytosis499
  • Author Index511
  • Subject Index537
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822309
  • ISBN-13 9780080496849
  • Author Abelson, John N.
  • Category Science
  • Subject Research & Methodology

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Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.
This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.