Elsevier S & T

Regulators and Effectors of Small GTPases, Part F: Ras Family I: Ras Family I

Abelson, John N.; Simon, Melvin I.

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Table of contents
  • Title Pageiii
  • Copyright Pageiv
  • Table of Contentsv
  • Contributors to Volume 332ix
  • Prefacexiii
  • Volumes in Seriesxv
  • Section I: Protein Expression and Protein–Protein Interactions1
  • Chapter 1. Mammalian Expression Vectors for Ras Family Proteins: Generation and Use of Expression Co3
  • Chapter 2. Protein Transduction: Delivery of Tat–GTPase Fusion Proteins into Mammalian Cells36
  • Chapter 3. Green Fluorescent Protein-Tagged Ras Proteins for Intracellular Localization50
  • Chapter 4. Targeting Proteins to Membranes, Using Signal Sequences for Lipid Modifications64
  • Chapter 5. Targeting Proteins to Specific Cellular Compartments to Optimize Physiological Activity77
  • Chapter 6. Mapping Protein–Protein Interactions with Alka- line Phosphatase Fusion Proteins88
  • Chapter 7. Assays of Human Postprenylation Processing En- zymes103
  • Chapter 8. In Vivo Prenylation Analysis of Ras and Rho Pro- teins115
  • Chapter 9. Ras Interaction with RalGDS Effector Targets127
  • Chapter 10. RAS Interaction with RIN1 Effector Target139
  • Chapter 11. Ras and Rap1 Interaction with AF-6 Effector Target151
  • Section II: Screening Analyses169
  • Chapter 12. Analysis of Protein Kinase Specificity by Peptide Libraries and Prediction of in Vivo Su171
  • Chapter 13. Peptide Library Screening for Determination of SH2 or Phosphotyrosine-Binding Domain Seq183
  • Chapter 14. Expression Cloning of Farnesylated Proteins195
  • Chapter 15. Expression Cloning to Identify Monomeric GTP- Binding Proteins by GTP Overlay203
  • Chapter 16. Retrovirus cDNA Expression Library Screening for Oncogenes211
  • Chapter 17. Identification of Ras-Regulated Genes by Representational Difference Analysis221
  • Chapter 18. Differential Display Analysis of Gene Expression Altered by ras Oncogene233
  • Chapter 19. cDNA Array Analyses of K-Ras-Induced Gene Transcription245
  • Chapter 20. Ras Signaling Pathway for Analysis of Protein–Protein Interactions260
  • Chapter 21. Isolation of Effector-Selective Ras Mutants by Yeast Two-Hybrid Screening270
  • Chapter 22. Two-Hybrid Dual Bait System to Discriminate Specificity of Protein Interactions in Small277
  • Chapter 23. Functional Proteomics Analysis of GTPase Signal- ing Networks300
  • Section III: Analyses of Mitogen-Activated Protein Kinase Cascades317
  • Chapter 24. Analyzing JNK and p38 Mitogen-Activated Protein Kinase Activity319
  • Chapter 25. Phospho-Specific Mitogen-Activated Protein Kinase Antibodies for ERK, JNK, and p38 Activ337
  • Chapter 26. Immunostaining for Activated Extracellular Signal-Regulated Kinases in Cells and Tissues343
  • Chapter 27. Dominant Negative Mutants of Mitogen-Activated Protein Kinase Pathway353
  • Chapter 28. Scaffold Protein Regulation of Mitogen-Activated Protein Kinase Cascade368
  • Chapter 29. Bacterial Expression of Activated Mitogen-Activated Protein Kinases387
  • Chapter 30. Steroid Receptor Fusion Proteins for Conditional Activation of Raf–MEK–ERK Signaling401
  • Chapter 31. Pharmacologic Inhibitors of MKK1 and MKK2417
  • Chapter 32. Analysis of Pharmacologic Inhibitors of Jun N-Ter- minal Kinases432
  • Author Index453
  • Subject Index477
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822330
  • ISBN-13 9780080522555
  • Author Abelson, John N.; Simon, Melvin I.
  • Category Science
  • Subject Research & Methodology

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The critically acclaimed laboratory standard for more than 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today, truly an essential publication for researchers in all fields of life sciences.
This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325 and 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.