Elsevier S & T

Regulators of G Protein Signalling, Part A

Siderovski, David

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Table of contents
  • Table of Contentsv
  • Contributors to Volume 389ix
  • Prefacexiii
  • Volumes in Seriesxvii
  • Section I: General Methods of RGS Protein Analysis Subsection A. Expression and Post-translational M1
  • Chapter 1. Quantitative Real-Time Polymerase Chain Reaction Measurement of Regulators of G-Protein S3
  • Chapter 2. Regulation of Chemokine-Induced Lymphocyte Migration by RGS Proteins15
  • Chapter 3. Role of Palmitoylation in RGS Protein Function33
  • Subsection B: Assays of RGS Box Activity and Allosteric Control56
  • Chapter 4. Fluorescence-Based Assays for RGS Box Function56
  • Chapter 5. Application of RGS Box Proteins to Evaluate G-Protein Selectivity in Receptor-Promoted Si71
  • Chapter 6. Allosteric Regulation of GAP Activity by Phospholipids in Regulators of G-Protein Signali89
  • Chapter 7. Assays of RGS Protein Modulation by Phosphatidylinositides and Calmodulin105
  • Subsection C. Electrophysiological Methods and RGS-Insensitive Galpha Subunits119
  • Chapter 8. Analyses of RGS Protein Control of Agonist- Evoked Ca2+ Signaling119
  • Chapter 9. Measuring the Modulatory Effects of RGS Proteins on GIRK Channels131
  • Chapter 10. Assays for G-Protein-Coupled Receptor Signaling Using RGS-Insensitive Galpha Subunits155
  • Chapter 11. Use of RGS-Insensitive Galpha Subunits to Study Endogenous RGS Protein Action on G-Prot170
  • Chapter 12. Endogenous RGS Proteins Regulate Presynaptic and Postsynaptic Function: Functional Expre190
  • Subsection D. Mouse Models of RGS Protein Action205
  • Chapter 13. In Situ Hybridization Analysis of RGS mRNA Regulation and Behavioral Phenotyping of RGS205
  • Chapter 14. RGS-Insensitive G-Protein Mutations to Study the Role of Endogenous RGS Proteins229
  • Subsection E. Methods of RGS Protein Inhibition244
  • Chapter 15. Ribozyme- and siRNA-Mediated Suppression of RGS-Containing RhoGEF Proteins244
  • Chapter 16. Structure-Based Design, Synthesis, and Activity of Peptide Inhibitors of RGS4 GAP Activi266
  • Chapter 17. Yeast-Based Screening for Inhibitors of RGS Proteins277
  • Section II: G-Protein Regulators of Model Organisms303
  • Chapter 18. Genetic Analysis of RGS Protein Function in Caenorhabditis elegans305
  • Chapter 19. Purification and In Vitro Functional Analysis of the Arabidopsis thaliana Regulator of G320
  • Chapter 20. AtRGS1 Function in Arabidopsis thaliana338
  • Chapter 21. Identification and Functional Analysis of the Drosophila Gene loco350
  • Chapter 22. Analysis of the Roles of Pins and Heterotrimeric G Proteins in Asymmetric Division of Dr364
  • Chapter 23. Mathematical Modeling of RGS and G-Protein Regulation in Yeast383
  • Chapter 24. Identification of Yeast Pheromone Pathway Modulators by High-Throughput Agonist Response399
  • Author Index411
  • Subject Index427
Book details
  • Vendor Elsevier S & T
  • SKU 9780121827946
  • ISBN-13 9780080497266
  • Author Siderovski, David
  • Category Medical
  • Subject Biochemistry

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This volume is an in-depth treatment of G-Protein Signaling, Part A, and will cover general methods of analysis of RGS protein analysis, including Expression and post-translational modification, Assays of GAP activity and allosteric control, Electrophysiological methods and RGS-insensitive Ga subunits, Mouse models of RGS protein action, Methods of RGS protein inhibition, and G-protein regulators of model organisms.

Table of Contents
-Expression and post-translational modification
-Assays of GAP activity and allosteric control
-Electrophysiological methods and RGS-insenstitive Ga subunits
-Mouse Models of RGS protein action
-Methods of RGS protein inhibition
-G-protein regulators of model organisms