Elsevier S & T

Ribonucleases, Part A: Functional Roles and Mechanisms of Action: Functional Roles and Mechanisms of Action

Nicholson, Allen W.

In stock
No reviews
74.000 KD inc. VAT
License
Table of contents
  • Table of Contentsv
  • Contributors to Volume 341ix
  • Prefacexv
  • Volume in Seriesxvii
  • Section I: Ribonuclease Classification and Review1
  • Chapter 1. A Natural Classification of Ribonucleases3
  • Chapter 2. The Ribonuclease T1 Family28
  • Chapter 3. Ribonuclease T242
  • Chapter 4. The Ribonuclease P Family56
  • Section II: Ribonuclease Assays79
  • Chapter 5. Fast, Facile, Hypersensitive Assays for Ribonucleolytic Activity81
  • Chapter 6. Activity Staining for Detection of Ribonucleases Using Dried Agarose Film Overlay Method94
  • Chapter 7. Gel Renaturation Assay for Ribonucleases113
  • Chapter 8. Analysis of Ribonucleases Following Gel Electrophoresis126
  • Chapter 9. Ribonuclease Assays Utilizing Toluidine Blue Indicator Plates, Methylene Blue, or Fluores142
  • Chapter 10. Ribonuclease Activities of Trypanosome RNA Editing Complex Directed to Cleave Specifical154
  • Chapter 11. Ribonuclease YI*, RNA Structure Studies, and Variable Single-Strand Specificities of RNa175
  • Section III: Secreted Ribonucleases187
  • Chapter 12. Bovine Pancreatic Ribonuclease A: Oxidative and Conformational Folding Studies189
  • Chapter 13. Purification of Engineered Human Pancreatic Ribonuclease221
  • Chapter 14. Degradation of Double-Stranded RNA by Mammalian Pancreatic-Type Ribonucleases234
  • Chapter 15. Seminal Ribonuclease: Preparation of Natural and Recombinant Enzyme, Quaternary Isoforms248
  • Chapter 16. Angiogenin263
  • Chapter 17. Eosinophil-Derived Neurotoxin273
  • Chapter 18. Eosinophil Cationic Protein287
  • Chapter 19. Deciphering the Mechanism of RNase T1305
  • Chapter 20. Mitogillin and Related Fungal Ribotoxins324
  • Chapter 21. RNase U2 and α-Sarcin: A Study of Relationships335
  • Chapter 22. Secretory Acid Ribonucleases from Tomato, Lycopersicon esculentum Mill.351
  • Chapter 23. Leczyme368
  • Section IV: Ribonucleases H375
  • Chapter 24. Prokaryotic Type 2 RNases H377
  • Chapter 25. RNase H1 of Saccharomyces cerevisiae: Methods and Nomenclature395
  • Chapter 26. Ribonucleases H of the Budding Yeast, Saccharomyces cerevisiae414
  • Chapter 27. Human RNases H430
  • Chapter 28. Assays for Retroviral RNase H440
  • Section V: Synthetic Ribonucleases453
  • Chapter 29. Sequence-Selective Artificial Ribonucleases455
  • Chapter 30. RNA Cleavage by 1,4-Diazabicyclo[2.2.2]- octane–Imidazole Conjugates468
  • Chapter 31. Preparation and Use of ZFY-6 Zinc Finger Ribonuclease490
  • Section VI: Ribonucleolytic Nucleic Acids501
  • Chapter 32. RNA Cleavage by the 10-23 DNA Enzyme503
  • Chapter 33. Leadzyme518
  • Chapter 34. Hammerhead Ribozyme Structure and Function in Plant RNA Replication540
  • Chapter 35. Kinetic Analysis of Bimolecular Hepatitis delta Ribozyme553
  • Chapter 36. Catalytic and Structural Assays for the Hairpin Ribozyme566
  • Chapter 37. Intracellular Applications of Ribozymes581
  • Section VII: Ribonuclease Inhibitors597
  • Chapter 38. Barnase–Barstar Interaction599
  • Chapter 39. Cytoplasmic Ribonuclease Inhibitor611
  • Chapter 40. Small Molecule Inhibitors of RNase A and Related Enzymes629
  • Chapter 41. Ribonuclease-Resistant RNA Controls and Standards648
  • Section VIII: Nonenzymatic Cleavage of RNA655
  • Chapter 42. Nonenzymatic Cleavage of Oligoribonucleotides657
  • Author Index677
  • Subject Index717
Book details
  • Vendor Elsevier S & T
  • SKU 9780121822422
  • ISBN-13 9780080496917
  • Author Nicholson, Allen W.
  • Category Science
  • Subject Biochemistry

Do you have questions about this book?

Ask an expert!

Description eBook delivery & access Reviews
This first of two volumes provides up-to-date, methods-related information on ribonuclease functions, assays, and applications. Chapter topics include the identification of, characterization of, and assays for secreted ribonucleases; viral ribonucleases, artificial and engineered ribonucleases, and ribozymes.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.